Construct: ORF TRCN0000488215
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021811.1_s317c1
- DNA Barcode:
- GGACTACGACGTGACCTAGTCAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM2 (2912)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488215
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2912 | GRM2 | glutamate metabotropic rece... | NM_000839.5 | 99.8% | 99.6% | 2063C>T;2066G>T;2203A>G |
| 2 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_017006271.1 | 99.8% | 99.6% | 2063C>T;2066G>T;2203A>G |
| 3 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533635.1 | 96.2% | 95.9% | (many diffs) |
| 4 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533636.1 | 79% | 78.7% | (many diffs) |
| 5 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533637.1 | 77.6% | 77.4% | (many diffs) |
| 6 | human | 2912 | GRM2 | glutamate metabotropic rece... | NM_001349116.2 | 56.5% | 56.3% | (many diffs) |
| 7 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533642.1 | 53.8% | 53.8% | 1410_1411ins1206 |
| 8 | human | 2912 | GRM2 | glutamate metabotropic rece... | NM_001349117.2 | 52.5% | 51.3% | (many diffs) |
| 9 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_024453490.1 | 49.3% | 49.1% | (many diffs) |
| 10 | human | 2912 | GRM2 | glutamate metabotropic rece... | XM_011533643.1 | 48.9% | 42.2% | (many diffs) |
| 11 | human | 2912 | GRM2 | glutamate metabotropic rece... | NR_146059.2 | 45% | (many diffs) | |
| 12 | mouse | 108068 | Grm2 | glutamate receptor, metabot... | NM_001160353.1 | 89.8% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2685
- ORF length:
- 2616
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggatcgctg cttgcgctcc tggcactgct gctgctgtgg ggtgctgtgg 121 ctgagggccc agccaagaag gtgctgaccc tggagggaga cttggtgctg ggtgggctgt 181 tcccagtgca ccagaagggc ggcccagcag aggactgtgg tcctgtcaat gagcaccgtg 241 gcatccagcg cctggaggcc atgctttttg cactggaccg catcaaccgt gacccgcacc 301 tgctgcctgg cgtgcgcctg ggtgcacaca tcctcgacag ttgctccaag gacacacatg 361 cgctggagca ggcactggac tttgtgcgtg cctcactcag ccgtggtgct gatggctcac 421 gccacatctg ccccgacggc tcttatgcga cccatggtga tgctcccact gccatcactg 481 gtgttattgg cggttcctac agtgatgtct ccatccaggt ggccaacctc ttgaggctat 541 ttcagatccc acagattagc tacgcctcta ccagtgccaa gctgagtgac aagtcccgct 601 atgactactt tgcccgcaca gtgcctcctg acttcttcca agccaaggcc atggctgaga 661 ttctccgctt cttcaactgg acctatgtgt ccactgtggc gtctgagggc gactatggcg 721 agacaggcat tgaggccttt gagctagagg ctcgtgcccg caacatctgt gtggccacct 781 cggagaaagt gggccgtgcc atgagccgcg cggcctttga gggtgtggtg cgagccctgc 841 tgcagaagcc cagtgcccgc gtggctgtcc tgttcacccg ttctgaggat gcccgggagc 901 tgcttgctgc cagccagcgc ctcaatgcca gcttcacctg ggtggccagt gatggttggg 961 gggccctgga gagtgtggtg gcaggcagtg agggggctgc tgagggtgct atcaccatcg 1021 agctggcctc ctaccccatc agtgactttg cctcctactt ccagagcctg gacccttgga 1081 acaacagccg gaacccctgg ttccgtgaat tctgggagca gaggttccgc tgcagcttcc 1141 ggcagcgaga ctgcgcagcc cactctctcc gggctgtgcc ctttgagcag gagtccaaga 1201 tcatgtttgt ggtcaatgca gtgtacgcca tggcccatgc gctccacaac atgcaccgtg 1261 ccctctgccc caacaccacc cggctctgtg acgcgatgcg gccagttaac gggcgccgcc 1321 tctacaagga ctttgtgctc aacgtcaagt ttgatgcccc ctttcgccca gctgacaccc 1381 acaatgaggt ccgctttgac cgctttggtg atggtattgg ccgctacaac atcttcacct 1441 atctgcgtgc aggcagtggg cgctatcgct accagaaggt gggctactgg gcagaaggct 1501 tgactctgga caccagcctc atcccatggg cctcaccctc agccggcccc ctgcccgcct 1561 ctcgctgcag tgagccctgc ctccagaatg aggtgaagag tgtgcagccg ggcgaagtct 1621 gctgctggct ctgcattccg tgccagccct atgagtaccg attggacgaa ttcacttgcg 1681 ctgattgtgg cctgggctac tggcccaatg ccagcctgac tggctgcttc gaactgcccc 1741 aggagtacat ccgctggggc gatgcctggg ctgtgggacc tgtcaccatc gcctgcctcg 1801 gtgccctggc caccctcttt gtgctgggtg tctttgtgcg gcacaatgcc acaccagtgg 1861 tcaaggcctc aggtcgggag ctctgctaca tcctgctggg tggtgtcttc ctctgctact 1921 gcatgacctt catcttcatt gccaagccat ccacggcagt gtgtacctta cggcgtcttg 1981 gtttgggcac tgccttctct gtctgctact cagccctgct caccaagacc aaccgcattg 2041 cacgcatctt cggtggggcc cgggagggtg cccagcggcc acgcttcatc agtcctgcct 2101 cacaggtggc catctgcctg gcacttatct tggtccagct gctcatcgtg gtcgcctggc 2161 tggtggtgga ggcaccgggc acaggcaagg agacagcccc cgaacggcgg gaggtggtga 2221 cactgcgctg caaccaccgc gatgcaagta tgttgggctc gctggcctac gatgtgctcc 2281 tcatcgcgct ctgcacgctt tatgccttca agactcgcaa gtgccccgaa aacttcaacg 2341 aggccaagtt cattggcttc accatgtaca ccaccTGCAT CATCTGGCTG GCATTCCTGC 2401 CCATCTTCTA TGTCACCTCC AGTGACTACC GGGTACAGAC CACCACCATG TGCGTGTCAG 2461 TCAGCCTCAG CGGCTCCGTG GTGCTTGGCT GCCTCTTTGC GCCCAAGCTG CACATCATCC 2521 TCTTCCAGCC GCAGAAGAAC GTGGTTAGCC ACCGGGCACC CACCAGCCGC TTTGGCAGTG 2581 CTGCTGCCAG GGCCAGCTCC AGCCTTGGCC AAGGGTCTGG CTCCCAGTTT GTCCCCACTG 2641 TTTGCAATGG CCGTGAGGTG GTGGACTCGA CAACGTCATC GCTTTGAGAC CCAGCTTTCT 2701 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2761 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2821 GTGGAAAGGA CGAGGACTAC GACGTGACCT AGTCAAGACG CGTTAAGTCg acaatcaacc 2881 tctggattac aaaatttgtg aaagatt