Construct: ORF TRCN0000488215

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021811.1_s317c1
DNA Barcode:
GGACTACGACGTGACCTAGTCAAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GRM2 (2912)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488215
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2912 GRM2 glutamate metabotropic rece... NM_000839.5 99.8% 99.6% 2063C>T;2066G>T;2203A>G
2 human 2912 GRM2 glutamate metabotropic rece... XM_017006271.1 99.8% 99.6% 2063C>T;2066G>T;2203A>G
3 human 2912 GRM2 glutamate metabotropic rece... XM_011533635.1 96.2% 95.9% (many diffs)
4 human 2912 GRM2 glutamate metabotropic rece... XM_011533636.1 79% 78.7% (many diffs)
5 human 2912 GRM2 glutamate metabotropic rece... XM_011533637.1 77.6% 77.4% (many diffs)
6 human 2912 GRM2 glutamate metabotropic rece... NM_001349116.2 56.5% 56.3% (many diffs)
7 human 2912 GRM2 glutamate metabotropic rece... XM_011533642.1 53.8% 53.8% 1410_1411ins1206
8 human 2912 GRM2 glutamate metabotropic rece... NM_001349117.2 52.5% 51.3% (many diffs)
9 human 2912 GRM2 glutamate metabotropic rece... XM_024453490.1 49.3% 49.1% (many diffs)
10 human 2912 GRM2 glutamate metabotropic rece... XM_011533643.1 48.9% 42.2% (many diffs)
11 human 2912 GRM2 glutamate metabotropic rece... NR_146059.2 45% (many diffs)
12 mouse 108068 Grm2 glutamate receptor, metabot... NM_001160353.1 89.8% 97.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2685
ORF length:
2616
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gggatcgctg cttgcgctcc tggcactgct gctgctgtgg ggtgctgtgg
121ctgagggccc agccaagaag gtgctgaccc tggagggaga cttggtgctg ggtgggctgt
181tcccagtgca ccagaagggc ggcccagcag aggactgtgg tcctgtcaat gagcaccgtg
241gcatccagcg cctggaggcc atgctttttg cactggaccg catcaaccgt gacccgcacc
301tgctgcctgg cgtgcgcctg ggtgcacaca tcctcgacag ttgctccaag gacacacatg
361cgctggagca ggcactggac tttgtgcgtg cctcactcag ccgtggtgct gatggctcac
421gccacatctg ccccgacggc tcttatgcga cccatggtga tgctcccact gccatcactg
481gtgttattgg cggttcctac agtgatgtct ccatccaggt ggccaacctc ttgaggctat
541ttcagatccc acagattagc tacgcctcta ccagtgccaa gctgagtgac aagtcccgct
601atgactactt tgcccgcaca gtgcctcctg acttcttcca agccaaggcc atggctgaga
661ttctccgctt cttcaactgg acctatgtgt ccactgtggc gtctgagggc gactatggcg
721agacaggcat tgaggccttt gagctagagg ctcgtgcccg caacatctgt gtggccacct
781cggagaaagt gggccgtgcc atgagccgcg cggcctttga gggtgtggtg cgagccctgc
841tgcagaagcc cagtgcccgc gtggctgtcc tgttcacccg ttctgaggat gcccgggagc
901tgcttgctgc cagccagcgc ctcaatgcca gcttcacctg ggtggccagt gatggttggg
961gggccctgga gagtgtggtg gcaggcagtg agggggctgc tgagggtgct atcaccatcg
1021agctggcctc ctaccccatc agtgactttg cctcctactt ccagagcctg gacccttgga
1081acaacagccg gaacccctgg ttccgtgaat tctgggagca gaggttccgc tgcagcttcc
1141ggcagcgaga ctgcgcagcc cactctctcc gggctgtgcc ctttgagcag gagtccaaga
1201tcatgtttgt ggtcaatgca gtgtacgcca tggcccatgc gctccacaac atgcaccgtg
1261ccctctgccc caacaccacc cggctctgtg acgcgatgcg gccagttaac gggcgccgcc
1321tctacaagga ctttgtgctc aacgtcaagt ttgatgcccc ctttcgccca gctgacaccc
1381acaatgaggt ccgctttgac cgctttggtg atggtattgg ccgctacaac atcttcacct
1441atctgcgtgc aggcagtggg cgctatcgct accagaaggt gggctactgg gcagaaggct
1501tgactctgga caccagcctc atcccatggg cctcaccctc agccggcccc ctgcccgcct
1561ctcgctgcag tgagccctgc ctccagaatg aggtgaagag tgtgcagccg ggcgaagtct
1621gctgctggct ctgcattccg tgccagccct atgagtaccg attggacgaa ttcacttgcg
1681ctgattgtgg cctgggctac tggcccaatg ccagcctgac tggctgcttc gaactgcccc
1741aggagtacat ccgctggggc gatgcctggg ctgtgggacc tgtcaccatc gcctgcctcg
1801gtgccctggc caccctcttt gtgctgggtg tctttgtgcg gcacaatgcc acaccagtgg
1861tcaaggcctc aggtcgggag ctctgctaca tcctgctggg tggtgtcttc ctctgctact
1921gcatgacctt catcttcatt gccaagccat ccacggcagt gtgtacctta cggcgtcttg
1981gtttgggcac tgccttctct gtctgctact cagccctgct caccaagacc aaccgcattg
2041cacgcatctt cggtggggcc cgggagggtg cccagcggcc acgcttcatc agtcctgcct
2101cacaggtggc catctgcctg gcacttatct tggtccagct gctcatcgtg gtcgcctggc
2161tggtggtgga ggcaccgggc acaggcaagg agacagcccc cgaacggcgg gaggtggtga
2221cactgcgctg caaccaccgc gatgcaagta tgttgggctc gctggcctac gatgtgctcc
2281tcatcgcgct ctgcacgctt tatgccttca agactcgcaa gtgccccgaa aacttcaacg
2341aggccaagtt cattggcttc accatgtaca ccaccTGCAT CATCTGGCTG GCATTCCTGC
2401CCATCTTCTA TGTCACCTCC AGTGACTACC GGGTACAGAC CACCACCATG TGCGTGTCAG
2461TCAGCCTCAG CGGCTCCGTG GTGCTTGGCT GCCTCTTTGC GCCCAAGCTG CACATCATCC
2521TCTTCCAGCC GCAGAAGAAC GTGGTTAGCC ACCGGGCACC CACCAGCCGC TTTGGCAGTG
2581CTGCTGCCAG GGCCAGCTCC AGCCTTGGCC AAGGGTCTGG CTCCCAGTTT GTCCCCACTG
2641TTTGCAATGG CCGTGAGGTG GTGGACTCGA CAACGTCATC GCTTTGAGAC CCAGCTTTCT
2701TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2761CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2821GTGGAAAGGA CGAGGACTAC GACGTGACCT AGTCAAGACG CGTTAAGTCg acaatcaacc
2881tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)