Construct: ORF TRCN0000488221
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021037.1_s317c1
- DNA Barcode:
- AACAACTTAGTTTTTTTGTTAAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR7 (3363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488221
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3363 | HTR7 | 5-hydroxytryptamine receptor 7 | NM_019860.4 | 100% | 100% | |
| 2 | human | 3363 | HTR7 | 5-hydroxytryptamine receptor 7 | NM_000872.5 | 97% | 97% | 1296_1335delinsG |
| 3 | human | 3363 | HTR7 | 5-hydroxytryptamine receptor 7 | NM_019859.4 | 90.1% | 90.1% | 1297_1437del |
| 4 | human | 3363 | HTR7 | 5-hydroxytryptamine receptor 7 | XM_024447973.1 | 48.8% | 48.8% | 0_1ins594;703_843del |
| 5 | human | 93164 | HTR7P1 | 5-hydroxytryptamine recepto... | NR_002774.3 | 26.2% | (many diffs) | |
| 6 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | XM_006526702.3 | 90.7% | 95.4% | (many diffs) |
| 7 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | NM_008315.2 | 88% | 92.6% | (many diffs) |
| 8 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | NM_001347442.1 | 83.9% | 88.2% | (many diffs) |
| 9 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | XM_017318067.1 | 47.3% | 51% | (many diffs) |
| 10 | mouse | 15566 | Htr7 | 5-hydroxytryptamine (seroto... | XM_006526704.3 | 45.1% | 48.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1368
- ORF length:
- 1296
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatggac gttaacagca gcggccgccc ggacctctac gggcacctcc 121 gctctttcct tctgccagaa gtggggcgcg ggctgcccga cttgagcccc gacggtggcg 181 ccgacccggt cgcgggctcc tgggcgccgc acctgctgag cgaggtgaca gccagcccgg 241 cgcccacctg ggacgcgccc ccggacaatg cctccggctg tggggaacag atcaactacg 301 gcagagtcga gaaagttgtg atcggctcca tcctgacgct catcacgctg ctgacgatcg 361 cgggcaactg cctggtggtg atctccgtgt gcttcgtcaa gaagctccgc cagccctcca 421 actacctgat cgtgtccctg gcgctggccg acctctcggt ggctgtggcg gtcatgccct 481 tcgtcagcgt caccgacctc atcgggggca agtggatctt tggacacttt ttctgtaatg 541 tcttcatcgc catggacgtc atgtgctgca cggcctcgat catgaccctg tgcgtgatca 601 gcattgacag gtaccttggg atcacaaggc ccctcacata ccctgtgagg cagaatggga 661 aatgcatggc gaagatgatt ctctccgtct ggcttctctc cgcctccatc accttacctc 721 cactctttgg atgggctcag aatgtaaatg atgataaggt gtgcttgatc agccaggact 781 ttggctatac gatttactct accgcagtgg cattttatat ccccatgtcc gtcatgcttt 841 tcatgtacta ccagatttac aaggctgcca ggaagagtgc tgccaaacac aagtttcctg 901 gcttccctcg agtggagcca gacagcgtca tcgccctgaa tggcatagtg aagctccaga 961 aggaggtgga agagtgtgca aacctttcga gactcctcaa gcatgaaagg aaaaacatct 1021 ccatctTTAA GCGAGAACAG AAAGCAGCCA CCACCCTGGG GATCATCGTC GGGGCCTTTA 1081 CCGTGTGCTG GCTGCCATTT TTCCTCCTCT CGACAGCCAG ACCCTTCATC TGTGGCACTT 1141 CCTGCAGCTG CATCCCACTG TGGGTGGAGA GGACATTTCT GTGGCTAGGC TATGCAAACT 1201 CTCTCATTAA CCCTTTTATA TATGCCTTCT TCAACCGGGA CCTGAGGACC ACCTATCGCA 1261 GCCTGCTCCA GTGCCAGTAC CGGAATATCA ACCGGAAGCT CTCAGCTGCA GGCATGCATG 1321 AAGCCCTGAA GCTTGCTGAG AGGCCAGAGA GACCTGAGTT TGTGCTGTAG AACCCAGCTT 1381 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1441 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1501 CTTGTGGAAA GGACGAAACA ACTTAGTTTT TTTGTTAAGT ACGCGTTAAG TCgacaatca 1561 acctctggat tacaaaattt gtgaaagatt