Transcript: Human NM_019860.4

Homo sapiens 5-hydroxytryptamine receptor 7 (HTR7), transcript variant b, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
HTR7 (3363)
Length:
3374
CDS:
369..1667

Additional Resources:

NCBI RefSeq record:
NM_019860.4
NBCI Gene record:
HTR7 (3363)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_019860.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000378339 CTGTTGTACACACTATCTTAT pLKO_005 1851 3UTR 100% 13.200 10.560 N HTR7 n/a
2 TRCN0000009124 CCAGGACTTTGGCTATACGAT pLKO.1 1070 CDS 100% 3.000 2.400 N HTR7 n/a
3 TRCN0000357503 GAATCCAGTCATCAGCTAATA pLKO_005 2201 3UTR 100% 13.200 9.240 N HTR7 n/a
4 TRCN0000378275 TCACCTTACCTCCACTCTTTG pLKO_005 1006 CDS 100% 10.800 7.560 N HTR7 n/a
5 TRCN0000009125 GTACCGGAATATCAACCGGAA pLKO.1 1574 CDS 100% 0.000 0.000 N HTR7 n/a
6 TRCN0000357452 TACTCTACCGCAGTGGCATTT pLKO_005 1092 CDS 100% 10.800 6.480 N HTR7 n/a
7 TRCN0000009123 GCTCAGAATGTAAATGATGAT pLKO.1 1032 CDS 100% 4.950 2.970 N HTR7 n/a
8 TRCN0000027449 GAAAGTTGTGATCGGCTCCAT pLKO.1 608 CDS 100% 2.640 1.584 N Htr7 n/a
9 TRCN0000027420 GCTATGCAAACTCTCTCATTA pLKO.1 1486 CDS 100% 13.200 6.600 Y Htr7 n/a
10 TRCN0000009122 GCACACCAACAGAACTGAGTT pLKO.1 1958 3UTR 100% 4.950 2.475 Y HTR7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019860.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488221 AACAACTTAGTTTTTTTGTTAAGT pLX_317 26.7% 100% 100% V5 (not translated due to prior stop codon) n/a
2 ccsbBroadEn_00808 pDONR223 100% 97% 97% None 1296_1296delGins40 n/a
3 ccsbBroad304_00808 pLX_304 0% 97% 97% V5 1296_1296delGins40 n/a
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