Construct: ORF TRCN0000488239
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021387.1_s317c1
- DNA Barcode:
- CGGAGTATGCGCATCATACGGTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MOXD1 (26002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488239
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 26002 | MOXD1 | monooxygenase DBH like 1 | XM_017010714.2 | 92.9% | 90.1% | (many diffs) |
2 | human | 26002 | MOXD1 | monooxygenase DBH like 1 | NM_015529.4 | 88.3% | 88.7% | 1_207del;1839_1840insTGAAAGCT |
3 | mouse | 59012 | Moxd1 | monooxygenase, DBH-like 1 | NM_021509.5 | 74.5% | 76.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1698
- ORF length:
- 1632
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatggc gtccgccgac atcgtcgtgg gcggggtggc ccacgggcgg ccctacctcc 121 aggattattt tacaaatgca aatagagagt tgaaaaaaga tgctcagcaa gattaccatc 181 tagaatatgc catggaaaat agcacacaca caataattga atttaccaga gagctgcata 241 catgtgacat aaatgacaag agtataacgg atagcactgt gagagtgatc tgggcctacc 301 accatgaaga tgcaggagaa gctggtccca agtaccatga ctccaatagg ggcaccaaga 361 gtttgcggtt attgaatcct gagaaaacta gtgtgctatc tacagcctta ccatactttg 421 atctggtaaa tcaggacgtc cccatcccaa acaaagatac aacatattgg tgccaaatgt 481 ttaagattcc tgtgttccaa gaaaagcatc atgtaataaa ggttgagcca gtgatacaga 541 gaggccatga gagtctggtg caccacatcc tgctctatca gtgcagcaac aactttaacg 601 acagcgttct ggagtccggc cacgagtgct atcaccccaa catgcccgat gcattcctca 661 cctgtgaaac tgtgattttt gcctgggcta ttggtggaga gggcttttct tatccacctc 721 atgttggatt atcccttggc actccattag atccgcatta tgtgctccta gaagtccatt 781 atgataatcc cacttatgag gaaggcttaa tagataattc tggactgagg ttattttaca 841 caatggatat aaggaaatat gatgctgggg tgattgaggc tggcctctgg gtgagcctct 901 tccataccat ccctccaggg atgcctgagt tccagtctga gggtcactgc actttggagt 961 gcctggaaga ggctctggaa gccgaaaagc caagtggaat tcatgtgttt gctgttcttc 1021 tccatgctca cctggctggc agaggcatca ggctgcgtca ttttcgaaaa gggaaggaaa 1081 tgaaattact tgcctatgat gatgattttg acttcaattt ccaggagttt cagtatctaa 1141 aggaagaaca aacaatctta ccaggagata acctaattac tgagtgtcgc tacaacacga 1201 aagatagagc tgagatgact tggggaggac taagcaccag gagtgaaatg tgtctctcat 1261 accttcttta ttacccaaga attaatctta ctcgatgtgc aagtattcca gacattatgg 1321 aacaacttca gttcattggg gttaaggaga tctacagacc agtcacgacc tggCCTTTCA 1381 TTATCAAAAG TCCCAAGCAA TATAAAAACC TTTCTTTCAT GGATGCTATG AATAAGTTTA 1441 AATGGACTAA AAAGGAAGGT CTCTCCTTCA ACAAGCTGGT CCTCAGCCTG CCAGTGAATG 1501 TGAGATGTTC CAAGACAGAC AATGCTGAGT GGTCGATTCA AGGAATGACA GCATTACCTC 1561 CAGATATAGA AAGACCCTAT AAAGCAGAAC CTTTGGTGTG TGGCACGTCT TCTTCCTCTT 1621 CCCTGCACAG AGATTTCTCC ATCAACTTGC TTGTTTGCCT TCTGCTACTC AGCTGCACGC 1681 TGAGCACCAA GAGCTTGTGA AAGCTTGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1741 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1801 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGGAGTAT 1861 GCGCATCATA CGGTCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1921 aagatt