Construct: ORF TRCN0000488242

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021104.1_s317c1
DNA Barcode:
GACCACCCAAAAAAGACTATCCAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ADGRB2 (576)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488242
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 576 ADGRB2 adhesion G protein-coupled ... NM_001364857.1 99.9% 100% 525C>T;4284G>A
2 human 576 ADGRB2 adhesion G protein-coupled ... XM_011541848.3 99.9% 100% 525C>T;4284G>A
3 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001899.1 99.9% 100% 525C>T;4284G>A
4 human 576 ADGRB2 adhesion G protein-coupled ... NM_001294335.2 99.8% 99.9% 525C>T;4284G>A;4414_4415insAGA
5 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001900.1 99.8% 99.9% 525C>T;4284G>A;4414_4415insAGA
6 human 576 ADGRB2 adhesion G protein-coupled ... XM_011541849.2 99.2% 99.2% 0_1ins36;489C>T;4248G>A
7 human 576 ADGRB2 adhesion G protein-coupled ... XM_024448673.1 99.2% 99.2% 0_1ins36;489C>T;4248G>A
8 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001901.1 98.7% 98.8% 525C>T;4284G>A;4515_4516ins57
9 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001902.1 98.6% 98.7% (many diffs)
10 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001903.1 98.5% 98.5% 525C>T;1985_1986ins69;4215G>A
11 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001904.1 97.8% 97.9% 525C>T;3353_3354ins99;4185G>A
12 human 576 ADGRB2 adhesion G protein-coupled ... NM_001294336.2 97.8% 97.8% (many diffs)
13 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001905.1 97.8% 97.8% (many diffs)
14 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001906.1 96.6% 96.7% (many diffs)
15 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001907.1 96.4% 96.5% 525C>T;1086_1087ins165;4119G>A
16 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001908.1 96.4% 96.5% 525C>T;919_920ins165;4119G>A
17 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001909.1 95.2% 95.3% (many diffs)
18 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001910.1 94.4% 94.4% (many diffs)
19 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001911.1 93.2% 93.2% (many diffs)
20 human 576 ADGRB2 adhesion G protein-coupled ... XM_017001912.1 93% 93% 525C>T;921_922ins330;3954G>A
21 human 576 ADGRB2 adhesion G protein-coupled ... XM_011541858.2 80.8% 80.8% 0_1ins909;3375G>A
22 human 576 ADGRB2 adhesion G protein-coupled ... XR_001737343.1 78.1% (many diffs)
23 mouse 230775 Adgrb2 adhesion G protein-coupled ... NM_001290715.1 89.5% 94.8% (many diffs)
24 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240508.1 89.5% 94.7% (many diffs)
25 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240507.1 88.3% 93.5% (many diffs)
26 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240506.1 88.3% 93.4% (many diffs)
27 mouse 230775 Adgrb2 adhesion G protein-coupled ... NM_173071.3 88% 93% (many diffs)
28 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240509.1 87.5% 92.7% (many diffs)
29 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240512.1 87.4% 92.7% (many diffs)
30 mouse 230775 Adgrb2 adhesion G protein-coupled ... NM_001199696.1 86.2% 91.3% (many diffs)
31 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240511.1 85.1% 90.1% (many diffs)
32 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240510.1 85% 90.1% (many diffs)
33 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240513.1 84.2% 89.3% (many diffs)
34 mouse 230775 Adgrb2 adhesion G protein-coupled ... NM_001290714.1 84.1% 89.3% (many diffs)
35 mouse 230775 Adgrb2 adhesion G protein-coupled ... XM_011240514.1 81.7% 86.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
4827
ORF length:
4755
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggagaat acaggttgga tgggcaaggg acataggatg accccagcct
121gtcccctctt actgtctgtg attctgtccc tgcgcctggc caccgccttc gaccccgccc
181ccagtgcctg ctctgccctg gcctcgggtg tgctctacgg ggccttctcg ctgcaggacc
241tctttcctac catcgcctcg ggctgctcct ggaccctgga gaaccctgac cccaccaagt
301actccctcta cctgcgcttc aaccgccagg agcaggtgtg cgcacacttt gccccccgcc
361tgctgcccct ggaccactac ctggtcaact ttacctgcct gcggcctagc cccgaggagg
421cggtggccca ggcggagtca gaggtggggc ggccagaaga ggaggaggca gaggcggcag
481cggggttgga gctgtgcagc ggctcaggcc cctttacctt cctgcacttc gacaagaact
541tcgtgcagct gtgcctgtcg gctgagccct ccgaggcccc gcgcctgctg gcgcctgctg
601ccctagcctt ccgctttgtc gaggtcttgc tcatcaacaa caacaactct agccaattca
661cctgtggtgt gctctgccgc tggagtgagg agtgtggccg cgctgccggc agggcctgcg
721gctttgctca gccaggctgc agctgccctg gagaggcggg ggccggctcc accaccacca
781catctccagg ccctcctgct gcccacaccc tgtccaatgc cctggtgccc gggggcccag
841ccccacctgc tgaggccgat ttgcactcgg ggagcagcaa tgatctgttc acaaccgaga
901tgagatatgg tgaggagccg gaagaggaac cgaaagtgaa aacccagtgg ccgaggtctg
961cagatgagcc tgggctatac atggcgcaga caggcgaccc ggcggctgag gagtggtccc
1021cgtggagcgt gtgttccctg acgtgtgggc agggtctgca ggtgcggacc cgctcctgtg
1081tgtcctcccc ctatgggacc ctgtgcagcg ggcccctgcg ggagaccagg ccctgcaaca
1141attcagccac ctgcccagtg cacggcgtgt gggaggagtg ggggtcctgg agcctgtgct
1201cccgcagctg cgggcggggg tcccggagcc ggatgcggac ctgcgtgccc ccccagcacg
1261gcggcaaggc ctgcgagggt cctgagctgc agactaagct ctgcagtatg gctgcctgcc
1321cggtggaagg ccagtggtta gaatggggtc cctggggccc atgctccacg tcctgtgcca
1381atgggaccca acagcgcagc cggaagtgca gcgtggcggg cccagcctgg gccacatgca
1441cgggtgccct cactgacacc cgggagtgca gcaacctcga gtgcccggcc actgatagca
1501agtgggggcc atggaatgcg tggagcctgt gctctaagac gtgtgacaca ggctggcagc
1561gccgcttccg catgtgccag gccacgggca cgcagggcta cccctgcgag ggcaccggag
1621aggaggtgaa gccttgtagt gagaagaggt gtccagcctt ccatgagatg tgcagggatg
1681agtacgtgat gctgatgacg tggaagaagg cagctgctgg cgagatcatc tacaacaagt
1741gccccccgaa tgcctcaggg tctgccagcc gccgctgtct cctcagtgcc caaggcgtgg
1801cgtactgggg gctgcccagc tttgctcgct gcatctccca tgagtaccgc tacctgtatc
1861tgtcacttag ggagcacctg gccaaggggc agcgcatgct ggcaggcgag ggcatgtcgc
1921aggtggtgcg cagcctgcag gagctactgg cccggcgcac ctactatagt ggggacctgc
1981tcttctctgt ggacattctg aggaatgtca ctgacacctt taagagggcc acctacgtgc
2041cctcggctga tgatgtgcag cgcttcttcc aggtggtgag cttcatggtg gatgcggaaa
2101acaaggagaa gtgggacgat gctcagcagg tgtcccctgg ctctgtgcac ctgctccgtg
2161tcgtggagga cttcattcac ctggtgggcg atgctctcaa ggccttccag agctctctga
2221ttgtcacaga taatctagtg atcagcattc agcgagagcc cgtctcagct gtgtccagtg
2281acatcacgtt ccccatgcgg ggccgccggg gcatgaagga ctgggtgcgg cactcagagg
2341accgcctctt cctgcccaag gaggtgctca gcctctcctc cccagggaag ccagccacat
2401ctggggcagc aggcagccct ggcaggggga ggggcccagg aacggtgcct cctggcccag
2461gccactccca ccagcgcctc ctcccagcag accctgatga gtcctcctac tttgtgatcg
2521gtgctgtact ctaccgcacc cttggcctca tcctgccgcc tcccaggccc ccgctggccg
2581tcacatcccg ggtgatgaca gtgactgtgc gcccccctac ccagcctcca gctgagcccc
2641tcatcactgt ggagctctcc tacatcatca atgggaccac ggatccccat tgcgccagct
2701gggactactc cagagcagat gccagctcag gagactggga cactgaaaat tgccagaccc
2761tggagaccca ggcagctcac acccgctgcc agtgccagca cctgtccacc tttgctgtac
2821tagcccagcc gcccaaggac ctgaccctgg agctggcggg ctccccctcg gtccccctgg
2881tgatcggctg tgcagtgtcg tgcatggcgc tgctcaccct gctcgccatc tatgccgcct
2941tttggaggtt cataaaatct gaacgctcca tcatcttgct gaacttctgc ctgtccatct
3001tggcatccaa catcctgatc ctcgtgggcc agtcccgggt gctgagcaag ggcgtgtgca
3061ccatgacggc tgccttcctg cacttcttct ttctctcctc cttttgctgg gtgcttaccg
3121aggcctggca gtcctacctg gctgtcattg ggcggatgcg cacccgcctc gttcgcaagc
3181gcttcctctg cctgggctgg ggtctgcctg ccctggtggt ggccgtgtct gttggcttta
3241cccgaacgaa aggatacggt acatccagct actgctggct ctccctggag ggcggcctgc
3301tctacgcctt tgtgggccct gcagccgtca ttgtcctggt gaacatgctc atcggaatca
3361tcgtcttcaa caagctcatg gcacgtgatg gcatctccga caaatccaag aagcagaggg
3421ccgggtcgga gcggtgcccc tgggccagcc tgctcctccc ctgctcagcg tgtggagcgg
3481tccccagccc cctgctcagc tcagcctcgg ccaggaacgc catggcctca ctctggagct
3541cctgcgtggt gctgcccctg ctggcgctca cctggatgtc tgccgtcctg gctatgacag
3601accgccgttc cgtcctcttc caggccctct ttgctgtctt caactccgcg cagggctttg
3661tcatcactgc tgtgcactgc ttcctgcgcc gagaggtcca ggatgtggtg aagtgccaga
3721tgggggtgtg ccgggctgat gagagcgaag actcccctga ctcgtgtaag aacgggcagc
3781tgcagatcct gtcagacttt gaaaaggatg tggatctggc ttgtcaaaca gtgctgttca
3841aggaggtcaa cacttgcaac ccgtccacca tcacgggcac actatcccgc ctgtccctgg
3901atgaggatga ggagcccaag tcctgcctcg tgggccctga gggcagcctc agcttctcac
3961cactgcctgg gaatatcctg gtgcccatgg cagcctcacc agggctgggg gagcctccgc
4021ccccacagga ggccaaccct gtttacatgt gtggggaggg tggcctgcgg cagctggacc
4081tcacatggct gcggcccact gagccaggct ctgagggaga ctacatggtg ctgccccggc
4141ggactttgag cctgcagcct ggcggtgggg gtggaggtgg tgaggatgcc cccagggccc
4201ggccggaggg gaccccccgg cgagctgcca agacagtggc ccacactgaa ggctacccca
4261gcttcctgtc cgtggaccac tcgggcctgg ggctgggccc tgcctatgga tctctccaga
4321atccctatgg aatgaccttc caaccgccac cgccaacacc cagcgcccgc caagtgcccg
4381agccagggga gcgcagccgg accatgcctc gcaccgtgcc cggctctacc atgaagatgg
4441gctccctgga gcgaaagaaa ttacggtatt cagacctgga ctttGAGAAG GTGATGCACA
4501CCCGGAAACG GCATTCAGAA CTCTACCACG AGCTCAACCA GAAGTTCCAC ACTTTCGACC
4561GCTACCGCAG CCAGTCCACG GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC TCGGGTGGGG
4621CAGCCGAGCG GAGCGTGTGC ACCGATAAGC CCAGCCCTGG GGAGCGCCCC AGCTTGTCCC
4681AACATCGGCG CCATCAGAGC TGGAGCACCT TCAAATCTAT GACACTGGGC TCGCTGCCCC
4741CCAAGCCCCG AGAACGGCTG ACTCTGCACC GGGCAGCAGC CTGGGAGCCC ACAGAACCAC
4801CGGATGGTGA CTTCCAGACA GAGGTGTGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA
4861TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
4921CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGACCA
4981CCCAAAAAAG ACTATCCATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
5041tgaaagatt

Download FASTA (ORF) (Full)