Construct: ORF TRCN0000488242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021104.1_s317c1
- DNA Barcode:
- GACCACCCAAAAAAGACTATCCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRB2 (576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | NM_001364857.1 | 99.9% | 100% | 525C>T;4284G>A |
2 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_011541848.3 | 99.9% | 100% | 525C>T;4284G>A |
3 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001899.1 | 99.9% | 100% | 525C>T;4284G>A |
4 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | NM_001294335.2 | 99.8% | 99.9% | 525C>T;4284G>A;4414_4415insAGA |
5 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001900.1 | 99.8% | 99.9% | 525C>T;4284G>A;4414_4415insAGA |
6 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_011541849.2 | 99.2% | 99.2% | 0_1ins36;489C>T;4248G>A |
7 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_024448673.1 | 99.2% | 99.2% | 0_1ins36;489C>T;4248G>A |
8 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001901.1 | 98.7% | 98.8% | 525C>T;4284G>A;4515_4516ins57 |
9 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001902.1 | 98.6% | 98.7% | (many diffs) |
10 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001903.1 | 98.5% | 98.5% | 525C>T;1985_1986ins69;4215G>A |
11 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001904.1 | 97.8% | 97.9% | 525C>T;3353_3354ins99;4185G>A |
12 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | NM_001294336.2 | 97.8% | 97.8% | (many diffs) |
13 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001905.1 | 97.8% | 97.8% | (many diffs) |
14 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001906.1 | 96.6% | 96.7% | (many diffs) |
15 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001907.1 | 96.4% | 96.5% | 525C>T;1086_1087ins165;4119G>A |
16 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001908.1 | 96.4% | 96.5% | 525C>T;919_920ins165;4119G>A |
17 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001909.1 | 95.2% | 95.3% | (many diffs) |
18 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001910.1 | 94.4% | 94.4% | (many diffs) |
19 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001911.1 | 93.2% | 93.2% | (many diffs) |
20 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_017001912.1 | 93% | 93% | 525C>T;921_922ins330;3954G>A |
21 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XM_011541858.2 | 80.8% | 80.8% | 0_1ins909;3375G>A |
22 | human | 576 | ADGRB2 | adhesion G protein-coupled ... | XR_001737343.1 | 78.1% | (many diffs) | |
23 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | NM_001290715.1 | 89.5% | 94.8% | (many diffs) |
24 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240508.1 | 89.5% | 94.7% | (many diffs) |
25 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240507.1 | 88.3% | 93.5% | (many diffs) |
26 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240506.1 | 88.3% | 93.4% | (many diffs) |
27 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | NM_173071.3 | 88% | 93% | (many diffs) |
28 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240509.1 | 87.5% | 92.7% | (many diffs) |
29 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240512.1 | 87.4% | 92.7% | (many diffs) |
30 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | NM_001199696.1 | 86.2% | 91.3% | (many diffs) |
31 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240511.1 | 85.1% | 90.1% | (many diffs) |
32 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240510.1 | 85% | 90.1% | (many diffs) |
33 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240513.1 | 84.2% | 89.3% | (many diffs) |
34 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | NM_001290714.1 | 84.1% | 89.3% | (many diffs) |
35 | mouse | 230775 | Adgrb2 | adhesion G protein-coupled ... | XM_011240514.1 | 81.7% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4827
- ORF length:
- 4755
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagaat acaggttgga tgggcaaggg acataggatg accccagcct 121 gtcccctctt actgtctgtg attctgtccc tgcgcctggc caccgccttc gaccccgccc 181 ccagtgcctg ctctgccctg gcctcgggtg tgctctacgg ggccttctcg ctgcaggacc 241 tctttcctac catcgcctcg ggctgctcct ggaccctgga gaaccctgac cccaccaagt 301 actccctcta cctgcgcttc aaccgccagg agcaggtgtg cgcacacttt gccccccgcc 361 tgctgcccct ggaccactac ctggtcaact ttacctgcct gcggcctagc cccgaggagg 421 cggtggccca ggcggagtca gaggtggggc ggccagaaga ggaggaggca gaggcggcag 481 cggggttgga gctgtgcagc ggctcaggcc cctttacctt cctgcacttc gacaagaact 541 tcgtgcagct gtgcctgtcg gctgagccct ccgaggcccc gcgcctgctg gcgcctgctg 601 ccctagcctt ccgctttgtc gaggtcttgc tcatcaacaa caacaactct agccaattca 661 cctgtggtgt gctctgccgc tggagtgagg agtgtggccg cgctgccggc agggcctgcg 721 gctttgctca gccaggctgc agctgccctg gagaggcggg ggccggctcc accaccacca 781 catctccagg ccctcctgct gcccacaccc tgtccaatgc cctggtgccc gggggcccag 841 ccccacctgc tgaggccgat ttgcactcgg ggagcagcaa tgatctgttc acaaccgaga 901 tgagatatgg tgaggagccg gaagaggaac cgaaagtgaa aacccagtgg ccgaggtctg 961 cagatgagcc tgggctatac atggcgcaga caggcgaccc ggcggctgag gagtggtccc 1021 cgtggagcgt gtgttccctg acgtgtgggc agggtctgca ggtgcggacc cgctcctgtg 1081 tgtcctcccc ctatgggacc ctgtgcagcg ggcccctgcg ggagaccagg ccctgcaaca 1141 attcagccac ctgcccagtg cacggcgtgt gggaggagtg ggggtcctgg agcctgtgct 1201 cccgcagctg cgggcggggg tcccggagcc ggatgcggac ctgcgtgccc ccccagcacg 1261 gcggcaaggc ctgcgagggt cctgagctgc agactaagct ctgcagtatg gctgcctgcc 1321 cggtggaagg ccagtggtta gaatggggtc cctggggccc atgctccacg tcctgtgcca 1381 atgggaccca acagcgcagc cggaagtgca gcgtggcggg cccagcctgg gccacatgca 1441 cgggtgccct cactgacacc cgggagtgca gcaacctcga gtgcccggcc actgatagca 1501 agtgggggcc atggaatgcg tggagcctgt gctctaagac gtgtgacaca ggctggcagc 1561 gccgcttccg catgtgccag gccacgggca cgcagggcta cccctgcgag ggcaccggag 1621 aggaggtgaa gccttgtagt gagaagaggt gtccagcctt ccatgagatg tgcagggatg 1681 agtacgtgat gctgatgacg tggaagaagg cagctgctgg cgagatcatc tacaacaagt 1741 gccccccgaa tgcctcaggg tctgccagcc gccgctgtct cctcagtgcc caaggcgtgg 1801 cgtactgggg gctgcccagc tttgctcgct gcatctccca tgagtaccgc tacctgtatc 1861 tgtcacttag ggagcacctg gccaaggggc agcgcatgct ggcaggcgag ggcatgtcgc 1921 aggtggtgcg cagcctgcag gagctactgg cccggcgcac ctactatagt ggggacctgc 1981 tcttctctgt ggacattctg aggaatgtca ctgacacctt taagagggcc acctacgtgc 2041 cctcggctga tgatgtgcag cgcttcttcc aggtggtgag cttcatggtg gatgcggaaa 2101 acaaggagaa gtgggacgat gctcagcagg tgtcccctgg ctctgtgcac ctgctccgtg 2161 tcgtggagga cttcattcac ctggtgggcg atgctctcaa ggccttccag agctctctga 2221 ttgtcacaga taatctagtg atcagcattc agcgagagcc cgtctcagct gtgtccagtg 2281 acatcacgtt ccccatgcgg ggccgccggg gcatgaagga ctgggtgcgg cactcagagg 2341 accgcctctt cctgcccaag gaggtgctca gcctctcctc cccagggaag ccagccacat 2401 ctggggcagc aggcagccct ggcaggggga ggggcccagg aacggtgcct cctggcccag 2461 gccactccca ccagcgcctc ctcccagcag accctgatga gtcctcctac tttgtgatcg 2521 gtgctgtact ctaccgcacc cttggcctca tcctgccgcc tcccaggccc ccgctggccg 2581 tcacatcccg ggtgatgaca gtgactgtgc gcccccctac ccagcctcca gctgagcccc 2641 tcatcactgt ggagctctcc tacatcatca atgggaccac ggatccccat tgcgccagct 2701 gggactactc cagagcagat gccagctcag gagactggga cactgaaaat tgccagaccc 2761 tggagaccca ggcagctcac acccgctgcc agtgccagca cctgtccacc tttgctgtac 2821 tagcccagcc gcccaaggac ctgaccctgg agctggcggg ctccccctcg gtccccctgg 2881 tgatcggctg tgcagtgtcg tgcatggcgc tgctcaccct gctcgccatc tatgccgcct 2941 tttggaggtt cataaaatct gaacgctcca tcatcttgct gaacttctgc ctgtccatct 3001 tggcatccaa catcctgatc ctcgtgggcc agtcccgggt gctgagcaag ggcgtgtgca 3061 ccatgacggc tgccttcctg cacttcttct ttctctcctc cttttgctgg gtgcttaccg 3121 aggcctggca gtcctacctg gctgtcattg ggcggatgcg cacccgcctc gttcgcaagc 3181 gcttcctctg cctgggctgg ggtctgcctg ccctggtggt ggccgtgtct gttggcttta 3241 cccgaacgaa aggatacggt acatccagct actgctggct ctccctggag ggcggcctgc 3301 tctacgcctt tgtgggccct gcagccgtca ttgtcctggt gaacatgctc atcggaatca 3361 tcgtcttcaa caagctcatg gcacgtgatg gcatctccga caaatccaag aagcagaggg 3421 ccgggtcgga gcggtgcccc tgggccagcc tgctcctccc ctgctcagcg tgtggagcgg 3481 tccccagccc cctgctcagc tcagcctcgg ccaggaacgc catggcctca ctctggagct 3541 cctgcgtggt gctgcccctg ctggcgctca cctggatgtc tgccgtcctg gctatgacag 3601 accgccgttc cgtcctcttc caggccctct ttgctgtctt caactccgcg cagggctttg 3661 tcatcactgc tgtgcactgc ttcctgcgcc gagaggtcca ggatgtggtg aagtgccaga 3721 tgggggtgtg ccgggctgat gagagcgaag actcccctga ctcgtgtaag aacgggcagc 3781 tgcagatcct gtcagacttt gaaaaggatg tggatctggc ttgtcaaaca gtgctgttca 3841 aggaggtcaa cacttgcaac ccgtccacca tcacgggcac actatcccgc ctgtccctgg 3901 atgaggatga ggagcccaag tcctgcctcg tgggccctga gggcagcctc agcttctcac 3961 cactgcctgg gaatatcctg gtgcccatgg cagcctcacc agggctgggg gagcctccgc 4021 ccccacagga ggccaaccct gtttacatgt gtggggaggg tggcctgcgg cagctggacc 4081 tcacatggct gcggcccact gagccaggct ctgagggaga ctacatggtg ctgccccggc 4141 ggactttgag cctgcagcct ggcggtgggg gtggaggtgg tgaggatgcc cccagggccc 4201 ggccggaggg gaccccccgg cgagctgcca agacagtggc ccacactgaa ggctacccca 4261 gcttcctgtc cgtggaccac tcgggcctgg ggctgggccc tgcctatgga tctctccaga 4321 atccctatgg aatgaccttc caaccgccac cgccaacacc cagcgcccgc caagtgcccg 4381 agccagggga gcgcagccgg accatgcctc gcaccgtgcc cggctctacc atgaagatgg 4441 gctccctgga gcgaaagaaa ttacggtatt cagacctgga ctttGAGAAG GTGATGCACA 4501 CCCGGAAACG GCATTCAGAA CTCTACCACG AGCTCAACCA GAAGTTCCAC ACTTTCGACC 4561 GCTACCGCAG CCAGTCCACG GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC TCGGGTGGGG 4621 CAGCCGAGCG GAGCGTGTGC ACCGATAAGC CCAGCCCTGG GGAGCGCCCC AGCTTGTCCC 4681 AACATCGGCG CCATCAGAGC TGGAGCACCT TCAAATCTAT GACACTGGGC TCGCTGCCCC 4741 CCAAGCCCCG AGAACGGCTG ACTCTGCACC GGGCAGCAGC CTGGGAGCCC ACAGAACCAC 4801 CGGATGGTGA CTTCCAGACA GAGGTGTGAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 4861 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 4921 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGACCA 4981 CCCAAAAAAG ACTATCCATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 5041 tgaaagatt