Transcript: Mouse NM_001290714.1

Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Adgrb2 (230775)
Length:
5185
CDS:
454..4875

Additional Resources:

NCBI RefSeq record:
NM_001290714.1
NBCI Gene record:
Adgrb2 (230775)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001290714.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415485 TGCACTTCGACAAGAACTTTG pLKO_005 878 CDS 100% 10.800 15.120 N Adgrb2 n/a
2 TRCN0000112158 CGGGATTATCGTCTTCAACAA pLKO.1 3552 CDS 100% 4.950 6.930 N Adgrb2 n/a
3 TRCN0000112156 GCACCAAAGGATATGGTACAT pLKO.1 3443 CDS 100% 4.950 6.930 N Adgrb2 n/a
4 TRCN0000425302 TGACCTGTTCACCACCGAGAT pLKO_005 1236 CDS 100% 4.050 3.240 N Adgrb2 n/a
5 TRCN0000426967 ACCACTACCTGGTCAACTTTA pLKO_005 719 CDS 100% 13.200 9.240 N Adgrb2 n/a
6 TRCN0000112155 CCAGCTTTGCTCGTTGCATAT pLKO.1 2006 CDS 100% 10.800 7.560 N Adgrb2 n/a
7 TRCN0000112157 CCACGAACTCAACCAGAAGTT pLKO.1 4626 CDS 100% 4.950 3.465 N Adgrb2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001290714.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489289 GCCGTAGACCGTAGGTACGATGTC pLX_317 7.6% 84.1% 89.2% V5 (many diffs) n/a
2 TRCN0000488242 GACCACCCAAAAAAGACTATCCAT pLX_317 7.4% 84.1% 89.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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