Construct: ORF TRCN0000488255

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF021981.1_s317c1
• DNA Barcode: ACGTTGGAAAGGGAAAGCTGGCTT
• Epitope Tag: V5 (not translated due to prior stop codon)
• Notes: Has stop codon in insert

Originally Annotated References:

• Gene: SMG1 (23049)

Vector Information:

• Vector Backbone: pLX_317
• Pol II Cassette 1: SV40-PuroR
• Pol II Cassette 2: EF1a-TRCN0000488255
• Selection Marker: PuroR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	23049	SMG1	SMG1 nonsense mediated mRNA...	NM_015092.4	99.9%	99.9%	(many diffs)
2	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_005255182.3	99.1%	99.2%	(many diffs)
3	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_011545769.3	99.1%	99.1%	(many diffs)
4	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_005255183.4	98.4%	98.2%	(many diffs)
5	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_017023066.2	98.4%	98.2%	(many diffs)
6	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_005255184.4	97.7%	97.8%	(many diffs)
7	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_024450198.1	97%	96.8%	(many diffs)
8	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_024450199.1	97%	96.8%	(many diffs)
9	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_011545770.1	82.1%	82.1%	(many diffs)
10	human	23049	SMG1	SMG1 nonsense mediated mRNA...	XM_017023067.1	82.1%	82.1%	(many diffs)
11	human	641298	SMG1P1	SMG1 pseudogene 1	NR_027154.1	38.6%		(many diffs)
12	human	100271836	SMG1P3	SMG1 pseudogene 3	NR_027155.2	38.5%		(many diffs)
13	human	100422558	SMG1P6	SMG1 pseudogene 6	NR_135312.1	26.9%		(many diffs)
14	human	440354	SMG1P2	SMG1 pseudogene 2	NR_135305.1	17.7%		(many diffs)
15	human	107984138	LOC107984138	serine/threonine-protein ki...	XM_017023965.2	17.5%	16.8%	(many diffs)
16	human	595101	SMG1P5	SMG1 pseudogene 5	NR_002453.5	17.3%		(many diffs)
17	human	440354	SMG1P2	SMG1 pseudogene 2	NR_135317.1	15.6%		(many diffs)
18	human	107984138	LOC107984138	serine/threonine-protein ki...	XM_017023966.2	11.3%	11%	(many diffs)
19	human	107282092	BOLA2-SMG1P6	BOLA2-SMG1P6 readthrough	NM_001320622.1	7.2%	5.7%	(many diffs)
20	human	107282092	BOLA2-SMG1P6	BOLA2-SMG1P6 readthrough	NM_001320624.1	7%	5.6%	(many diffs)
21	human	107282092	BOLA2-SMG1P6	BOLA2-SMG1P6 readthrough	NM_001320623.1	6.6%	4.9%	(many diffs)
22	human	107282092	BOLA2-SMG1P6	BOLA2-SMG1P6 readthrough	NM_001320627.1	5.2%	5.1%	(many diffs)
23	human	107282092	BOLA2-SMG1P6	BOLA2-SMG1P6 readthrough	NM_001320628.1	5.2%	5.1%	(many diffs)
24	human	107282092	BOLA2-SMG1P6	BOLA2-SMG1P6 readthrough	NM_001320625.1	4.4%	4.3%	(many diffs)
25	mouse	233789	Smg1	SMG1 homolog, phosphatidyli...	NM_001031814.1	90.4%	97.6%	(many diffs)
26	mouse	233789	Smg1	SMG1 homolog, phosphatidyli...	XM_006507677.2	90.4%	97.6%	(many diffs)
27	mouse	233789	Smg1	SMG1 homolog, phosphatidyli...	XM_006507678.2	89.7%	96.7%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

108

ORF end:

11091

ORF length:

10983

Sequence:

1	tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61	caggctccgc ggccgccccc ttcaccgtcg acgtgtcggg gaaggtaatg agccgcagag
121	ccccggggtc tcggctgagc agcggaggcg gcggcggggg caccaagtat ccgcggagct
181	ggaatgactg gcaacccaga actgatagtg catcagccga cccagataat ttaaaatatt
241	cttcatccag agatagaggt ggttcttcct cttatggact gcaaccttca aattcagctg
301	tggtgtctcg gcaaaggcac gatgatacca gagtccacgc tgacatacag aatgacgaaa
361	agggtggcta cagtgtcaat ggaggatctg gggaaaatac ttatggtcgg aagtcgttgg
421	ggcaagagct gagggttaac aatgtgacca gccctgagtt caccagtgtt cagcatggca
481	gtcgtgcttt agccaccaaa gacatgagga aatcacagga gagatcgatg tcttattctg
541	atgagtctcg actgtcgaat cttcttcgga ggatcacccg ggaagacgac agagaccgaa
601	gattggctac tgtaaagcag ttgaaagaat ttattcagca accagaaaat aagctggtac
661	tagttaaaca attggataat atcttggctg ctgtacatga cgtgcttaat gaaagtagca
721	aattgcttca ggagttgaga caggagggag cttgctgtct tggccttctt tgtgcttctc
781	tgagctatga ggctgagaag atcttcaagt ggatttttag caaatttagc tcatctgcaa
841	aagatgaagt taaactcctc tacttatgtg ccacctacaa agcactagag actgtaggag
901	aaaagaaagc cttttcatct gtaatgcagc ttgtaatgac cagcctgcag tctattcttg
961	aaaatgtgga tacaccagaa ttgctttgta aatgtgttaa gtgcattctt ttggtggctc
1021	gatgttaccc tcatattttc agcactaatt ttagggatac agttgatata ttagttggat
1081	ggcatataga tcatactcag aaaccttcgc tcacgcagca ggtatctggg tggttgcaga
1141	gtttggagcc attttgggta gctgatcttg cattttctac tactcttctt ggtcagtttc
1201	tggaagacat ggaagcatat gctgaggacc tcagccatgt ggcctctggg gaatcagtgg
1261	atgaagatgt ccctcctcca tcagtgtcat taccaaagct ggctgcactt ctccgggtat
1321	ttagtactgt ggtgaggagc attggggaac gcttcagccc aattcggggt cctccaatta
1381	ctgaggcata tgtaacagat gttctgtaca gagtaatgag atgtgtgacg gctgcaaacc
1441	aggtgttttt ttctgaggct gtgttgacag ctgctaatga gtgtgttggt gttttgctcg
1501	gcagcttgga tcctagcatg actatacatt gtgacatggt cattacatat ggattagacc
1561	aactggagaa ttgccagact tgtggtaccg attatatcat ctcagtcttg aatttactca
1621	cgctgattgt tgaacagata aatacgaaac tgccatcatc atttgtagaa aaactgttta
1681	taccatcatc taaactacta ttcttgcgtt atcataaaga aaaagaggtt gttgctgtag
1741	cccatgctgt ttatcaagca gtgctcagct tgaagaatat tcctgttttg gagactgcct
1801	ataagttaat attgggagaa atgacttgtg ccctaaacaa cctcctacac agtctacaac
1861	ttcctgaggc ctgttctgaa ataaaacatg aggcttttaa gaatcatgtg ttcaatgtag
1921	acaatgcaaa atttgtagtt atatttgacc tcagtgccct gactacaatt ggaaatgcca
1981	aaaactcact aatagggatg tgggcgctat ctccaactgt ctttgcactt ctgagtaaga
2041	atctgatgat tgtgcacagt gacctggctg ttcacttccc tgccattcag tatgctgtgc
2101	tctacacatt gtattctcat tgtaccaggc atgatcactt tatctctagt agcctcagtt
2161	cttcctctcc ttctttgttt gatggagctg tgattagcac tgtaactacg gctacaaaga
2221	aacatttctc aattatatta aatcttctgg gaatattact taagaaagat aaccttaacc
2281	aggacacgag gaaactgtta atgacttggg ctttggaagc agctgtttta atgaagaagt
2341	ctgaaacata cgcaccttta ttctctcttc cgtctttcca taaattttgc aaaggccttt
2401	tagccaacac tctcgttgaa gatgtgaata tctgtctgca ggcatgcagc agtctacatg
2461	ctctgtcctc ttccttgcca gatgatcttt tacagagatg tgtcgatgtt tgccgtgttc
2521	aactagtgca cagtggaact cgtattcgac aagcatttgg aaaactgttg aaatcaattc
2581	ctttagatgt tgtcctaagc aataacaatc acacagaaat tcaagaaatt tctttagcat
2641	taagaagtca catgagtaaa gcaccaagta atacattcca cccccaagat ttctctgatg
2701	ttattagttt tattttgtat gggaactctc atagaacagg gaaggacaat tggttggaaa
2761	gactgttcta tagctgccag agactggata agcgtgacca gtcaacaatt ccacgcaatc
2821	tcctgaagac agatgctgtc ctttggcagt gggccatatg ggaagctgca caattcactg
2881	ttctttctaa gctgagaacc ccactgggca gagctcaaga caccttccag acaattgaag
2941	gtatcattcg aagtctcgca gctcacacat taaaccctga tcaggatgtt agtcagtgga
3001	caactgcaga caatgatgaa ggccatggta acaaccaact tagacttgtt cttcttctgc
3061	agtatctgga aaatctggag aaattaatgt ataatgcata cgagggatgt gctaatgcat
3121	taacttcacc tcccaaggtc attagaactt ttttctatac caatcgccaa acttgtcagg
3181	actggctaac gcggattcga ctctccatca tgagggtagg attgttggca ggccagcctg
3241	cagtgacagt gagacatggc tttgacttgc ttacagagat gaaaacaacc agcctatctc
3301	aggggaatga attggaagta accattatga tggtggtaga agcattatgt gaacttcatt
3361	gtcctgaagc tatacaggga attgctgtct ggtcatcatc tattgttgga aaaaatcttc
3421	tgtggattaa ctcagtggct caacaggctg aagggaggtt tgaaaaggcc tctgtggagt
3481	accaggaaca cctgtgtgcc atgacaggtg ttgattgctg catctccagc tttgacaaat
3541	cggtgctcac cttagccaat gctgggcgta acagtgccag cccgaaacat tctctgaatg
3601	gtgaatccag aaaaactgtg ctgtccaaac cgactgactc ttcccctgag gttataaatt
3661	atttaggaaa taaagcatgt gagtgctaca tctcaattgc cgattgggct gctgtgcagg
3721	aatggcagaa cgctatccat gacttgaaaa agagtaccag tagcacttcc ctcaacctga
3781	aagctgactt caactatata aaatcattaa gcagctttga gtctggaaaa tttgttgaat
3841	gtaccgagca gttagaattg ttaccaggag aaaatatcaa tctacttgct ggaggatcaa
3901	aagaaaaaat agacatgaaa aaactgcttc ctaacatgtt aagtccggat ccgagggaac
3961	ttcagaaatc cattgaagtt caattgttaa gaagttctgt ttgtttggca actgctttaa
4021	acccgataga acaagatcag aagtggcagt ctataactga aaatgtggta aagtacttga
4081	agcaaacatc ccgcatcgct attggacctc tgagactttc tactttaaca gtttcacagt
4141	ctttgccagt tctaagtacc ttgcagctgt attgctcatc tgctttggag aacacagttt
4201	ctaacagact ttcaacagag gactgtctta ttccactctt cagtgaagct ttacgttcat
4261	gtaaacagca tgacgtgagg ccatggatgc aggcattaag gtatactatg taccagaatc
4321	agttgttgga gaaaattaaa gaacaaacag tcccaattag aagccatctc atggaattag
4381	gtctaacagc agcaaaattt gctagaaaac gagggaatgt gtcccttgca acaagactgc
4441	tggcacagtg cagtgaagtt cagctgggaa agaccaccac tgcacaggat ttagtccaac
4501	attttaaaaa actatcaacc caaggtcaag tggatgaaaa atgggggccc gaacttgata
4561	ttgaaaaaac caaattgctt tatacagcag gccagtcaac acatgcaatg gaaatgttga
4621	gttcttgtgc catatctttc tgcaagtctg tgaaagctga atatgcagtt gctaaatcaa
4681	ttctgacact ggctaaatgg atccaggcag aatggaaaga gatttcagga cagctgaaac
4741	aggtttacag agctcagcac caacagaact tcacaggtct ttctactttg tctaaaaaca
4801	tactcactct aatagaactg ccatctgtta atacgatgga agaagagtat cctcggatcg
4861	agagtgaatc tacagtgcat attggagttg gagaacctga cttcattttg ggacagttgt
4921	atcacctgtc ttcagtacag gcacctgaag tagccaaatc ttgggcagcg ttggccagct
4981	gggcttatag gtggggcaga aaggtggttg acaatgccag tcagggagaa ggtgttcgtc
5041	tgctgcctag agaaaaatct gaagttcaga atctacttcc agacactata actgaggaag
5101	agaaagagag aatatatggt attcttggac aggctgtgtg tcggccggcg gggattcagg
5161	atgaagatat aacacttcag ataactgaga gtgaagacaa cgaagaagat gacatggttg
5221	atgttatctg gcgtcagttg atatcaagct gcccatggct ttcagaactt gatgaaagtg
5281	caactgaagg agttattaaa gtgtggagga aagttgtaga tagaatattc agcctgtaca
5341	aactctcttg cagtgcatac tttactttcc ttaaactcaa cgctggtcaa attcctttag
5401	atgaggatga ccctaggctg catttaagtc acagagtgga acagagcact gatgacatga
5461	ttgtgatggc cacattgcgc ctgctgcggt tgctcgtgaa gcatgctggt gagcttcggc
5521	agtatctgga gcacggcttg gagacaacac ccactgcacc atggagagga attattccgc
5581	aacttttctc acgcttaaac caccctgaag tgtatgtgcg ccaaagtatt tgtaaccttc
5641	tctgccgtgt ggctcaagat tccccacatc tcatattgta tcctgcaata gtgggtacca
5701	tatcgcttag tagtgaatcc caggcttcag gaaataaatt ttccactgca attccaactt
5761	tacttggcaa tattcaagga gaagaattgc tggtttctga atgtgaggga ggaagtcctc
5821	ctgcatctca ggatagcaat aaggatgaac ctaaaagtgg attaaatgaa gaccaagcca
5881	tgatgcagga ttgttacagc aaaattgtag ataagctgtc ctctgcaaac cccaccatgg
5941	tattacaggt tcagatgctc gtggctgaac tgcgcagggt cactgtgctc tgggatgagc
6001	tctggctggg agttttgctg caacaacaca tgtatgtcct gagacgaatt cagcagcttg
6061	aagatgaggt gaagagagtc cagaacaaca acaccttacg caaagaagag aaaattgcaa
6121	tcatgaggga gaagcacaca gctttgatga agcccatcgt atttgctttg gagcatgtga
6181	ggagtatcac agcggctcct gcagaaacac ctcatgaaaa atggtttcag gataactatg
6241	gtgatgccat tgaaaatgcc ctagaaaaac tgaagactcc attgaaccct gcaaagcctg
6301	ggagcagctg gattccattt aaagagataa tgctaagttt gcaacagaga gcacagaagc
6361	gtgcaagtta catcttgcgt cttgaagaaa tcagtccatg gttggctgcc atgactaaca
6421	ctgaaattgc tcttcctggg gaagtctcag ccagagacac tgtcacaatc catagtgtgg
6481	gcggaaccat cacaatctta ccgactaaaa ccaagccaaa gaaacttctc tttcttggat
6541	cagatgggaa gagctatcct tatcttttca aaggactgga ggatttacat ctggatgaga
6601	gaataatgca gttcctatct attgtgaata ccatgtttgc tacaattaat cgccaagaaa
6661	caccccggtt ccatgctcga cactattctg taacaccact aggaacaaga tcaggactaa
6721	tccagtgggt agatggagcc acacccttat ttggtcttta caaacgatgg caacaacggg
6781	aagctgcctt acaagcacaa aaggcccaag attcctacca aactcctcag aatcctggaa
6841	ttgtaccccg tcctagtgaa ctttattaca gtaaaattgg ccctgctttg aaaacagttg
6901	ggcttagcct ggatgtgtcc cgtcgggatt ggcctcttca tgtaatgaag gcagtattgg
6961	aagagttaat ggaggccaca cccccgaatc tccttgccaa agagctctgg tcatcttgca
7021	caacacctga tgaatggtgg agagttacgc agtcttatgc aagatctact gcagtcatgt
7081	ctatggttgg atacataatt ggccttggag ccagacatct ggataatgtt cttatagata
7141	tgacgactgg agaagttgtt cacatagatt acaatgtttg ctttgaaaaa ggtaaaagcc
7201	ttagagttcc tgagaaagta ccttttcgaa tgacacaaaa cattgaaaca gcactgggtg
7261	taactggagt agaaggtgta tttaggcttt catgtgagca ggttttacac attatgcggc
7321	gtggcagaga gaccctgctg acgctgctgg aggcctttgt gtacgaccct ctggtggact
7381	ggacagcagg aggcgaggct gggtttgctg gtgctgtcta tggtggaggt ggccagcagg
7441	ccgagagcaa gcagagcaag agagagatgg agcgagagat cacccgcagc ctgttttctt
7501	ctagagtagc tgagattaag gtgaactggt ttaagaatag agatgagatg ctggttgtgc
7561	ttcccaagtt ggacggtagc ttagatgaat acctaagctt gcaagagcaa ctgacagatg
7621	tggaaaaact gcagggcaaa ctactggagg aaatagagtt tctagaagga gctgaagggg
7681	tggatcatcc ttctcatact ctgcaacaca ggtattctga gcacacccaa ctacagactc
7741	agcaaagagc tgttcaggaa gcaatccagg tgaagctgaa tgaatttgaa caatggataa
7801	cacattatca ggctgcattc aataatttag aagcaacaca gcttgcaagc ttgcttcaag
7861	agataagcac acaaatggac cttggtcctc caagttacgt gccagcaaca gcctttctgc
7921	agaatgctgg tcaggcccac ttgattagcc agtgcgagca gctggagggg gaggttggtg
7981	ctctcctgca gcagaggcgc tccgtgctcc gtggctgtct ggagcaactg catcactatg
8041	caaccgtggc cctgcagtat ccgaaggcca tatttcagaa acatcgaatt gaacagtgga
8101	agacctggat ggaagagctc atctgtaaca ccacagtaga gcgttgtcaa gagctctata
8161	ggaaatatga aatgcaatat gctccccagc cacccccaac agtgtgtcag ttcatcactg
8221	ccactgaaat gaccctgcag cgatacgcag cagacatcaa cagcagactt attagacaag
8281	tggaacgctt gaaacaggaa gctgtcactg tgccagtttg tgaagatcag ttgaaagaaa
8341	ttgaacgttg cattaaagtt ttccttcatg agaatggaga agaaggatct ttgagtctag
8401	caagtgttat tatttctgcc ctttgtaccc ttacaaggcg taacctgatg atggaaggtg
8461	cagcgtcaag tgctggagaa cagctggttg atctgacttc tcgggatgga gcctggttct
8521	tggaggaact ctgcagtatg agcggaaacg tcacctgctt ggttcagtta ctgaagcagt
8581	gccacctggt gccacaggac ttagatatcc cgaaccccat ggaagcgtct gagacagttc
8641	acttagccaa tggagtgtat acctcacttc aggaattgaa ttcgaatttc cggcaaatca
8701	tatttccaga agcacttcga tgtttaatga aaggggaata cacgttagaa agtatgctgc
8761	atgaactgga cggtcttatt gagcagacca ccgatggcgt tcccctgcag actctagtgg
8821	aatctcttca ggcctactta agaaacgcag ctatgggact ggaagaagaa acacatgctc
8881	attacatcga tgttgccaga ctactacatg ctcagtacgg tgaattaatc caaccgagaa
8941	atggttcagt tgatgaaaca cccaaaatgt cagctggcca gatgcttttg gtagcattcg
9001	atggcatgtt tgctcaagtt gaaactgctt tcagcttatt agttgaaaag ttgaacaaga
9061	tggaaattcc catagcttgg cgaaagattg acatcataag ggaagccagg agtactcaag
9121	ttaatttttt tgatgatgat aatcaccggc aggtgctaga agagattttc tttctaaaaa
9181	gactacagac tattaaggag ttcttcaggc tctgtggtac cttttctaaa acattgtcag
9241	gatcaagttc acttgaagat cagaatactg tgaatgggcc tgtacagatt gtcaatgtga
9301	aaaccctttt tagaaactct tgtttcagtg aagaccaaat ggccaaacct atcaaggcat
9361	tcacagctga ctttgtgagg cagctcttga tagggctacc caaccaagcc ctcggactca
9421	cactgtgcag ttttatcagt gctctgggtg tagacatcat tgctcaagta gaggcaaagg
9481	actttggtgc cgaaagcaaa gtttctgttg atgatctctg taagaaagcg gtggaacata
9541	acatccagat agggaagttc tctcagctgg ttatgaacag ggcaactgtg ttagcaagtt
9601	cttacgacac tgcctggaag aagcatgact tggtgcgaag gctagaaacc agtatttctt
9661	cttgtaagac aagcctgcag cgggttcagc tgcatattgc catgtttcag tggcaacatg
9721	aagatctact tatcaataga ccacaagcca tgtcagtcac acctccccca cggtctgcta
9781	tcctaaccag catgaaaaag aagctgcata ccctgagcca gattgaaact tctattgcaa
9841	cagttcagga gaagctagct gcacttgaat caagtattga acagcgactc aagtgggcag
9901	gtggtgccaa ccctgcattg gcccctgtac tacaagattt tgaagcaacg atagctgaaa
9961	gaagaaatct tgtccttaaa gagagccaaa gagcaagtca ggtcacattt ctctgcagca
10021	atatcattca ttttgaaagt ttacgaacaa gaactgcaga agccttaaac ctggatgcgg
10081	cgttatttga actaatcaag cgatgtcagc agatgtgttc gtttgcatca cagtttaaca
10141	gttcagtgtc tgagttagag cttcgtttat tacagagagt ggacactggt cttgaacatc
10201	ctattggcag ctctgaatgg cttttgtcag cacacaaaca gttgacccag gatatgtcta
10261	ctcagagggc aattcagaca gagaaagagc agcagataga aacggtctgt gaaacaattc
10321	agaatctggt tgataatata aagactgtgc tcactggtca taaccgacag cttggagatg
10381	tcaaacatct cttgaaagct atggctaagg atgaagaagc tgctctggca gatggtgaag
10441	atgttcccta tgagaacagt gttaggcagt ttttgggtga atataaatca tggcaagaca
10501	acattcaaac agttctattt acattagtcc aggctatggg tcaggttcga agtcaagaac
10561	acgttgaaat gctccaggaa atcactccca ccttgaaaga actgaaaaca caaagtcaga
10621	gtatctataa taatttagtg agttttgcat cacccttagt caccgatgca acaaatgaat
10681	gttcgagtcc aacgtcatct gctacttatc agccatcctt cgctgcagca gtccggagta
10741	acactggcca gaagactcag cctgatgtca tgtcacagaa tgctagaaag ctgatccaga
10801	aaaatcttgc tacatcagct gatactccac caagcaccgt tccaggaact ggcaagagtg
10861	ttgcttgtag tcctaaaaag gcagtcagag accctaaaac tgggaaagcg gtgcaagaga
10921	gaaactccTA TGCAGTGAGT GTGTGGAAGA GAGTGAAAGC CAAGTTAGAG GGCCGAGATG
10981	TTGATCCGAA TAGGAGGATG TCAGTTGCTG AACAGGTTGA CTATGTCATT AAGGAAGCAA
11041	CTAATCTAGA TAACTTGGCT CAGCTGTATG AAGGTTGGAC AGCCTGGGTG TGAAAGGGTG
11101	GGCGCGCCGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
11161	CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
11221	TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACGTTG GAAAGGGAAA GCTGGCTTAC
11281	GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

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