Construct: ORF TRCN0000488279

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020750.1_s317c1
DNA Barcode:
TCCTTGCACTAACCTTCTTCTCTA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
FLT3 (2322)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488279
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2322 FLT3 fms related tyrosine kinase 3 NM_004119.2:c.2503G>C 99.9% 100% 288C>T
2 human 2322 FLT3 fms related tyrosine kinase 3 NM_004119.3 99.9% 99.8% 288C>T;2503G>C
3 human 2322 FLT3 fms related tyrosine kinase 3 NM_004119.2:c.2508_2510del 99.8% 99.7% 288C>T;2503G>C;2505_2506insATC
4 human 2322 FLT3 fms related tyrosine kinase 3 XM_011535015.2 98% 97.9% 0_1ins57;231C>T;2446G>C
5 human 2322 FLT3 fms related tyrosine kinase 3 XM_017020486.1 92.6% 92.6% 288C>T;740_741ins216;2287G>C
6 human 2322 FLT3 fms related tyrosine kinase 3 XM_011535017.2 82.3% 82.2% 0_1ins525;1978G>C
7 human 2322 FLT3 fms related tyrosine kinase 3 XM_011535018.2 82.3% 82.2% 0_1ins525;1978G>C
8 human 2322 FLT3 fms related tyrosine kinase 3 XM_017020487.1 82.3% 82.2% 0_1ins525;1978G>C
9 human 2322 FLT3 fms related tyrosine kinase 3 NR_130706.2 75.2% (many diffs)
10 human 2322 FLT3 fms related tyrosine kinase 3 XM_017020488.1 70.4% 67.8% 0_1ins803;77_78ins76;1624G>C
11 human 2322 FLT3 fms related tyrosine kinase 3 XM_017020489.1 69.8% 69.7% 0_1ins897;1606G>C
12 mouse 14255 Flt3 FMS-like tyrosine kinase 3 NM_010229.2 82.4% 85.3% (many diffs)
13 mouse 14255 Flt3 FMS-like tyrosine kinase 3 XM_006504804.3 51.5% 53% (many diffs)
14 mouse 14255 Flt3 FMS-like tyrosine kinase 3 XM_006504805.3 47.6% 50.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3051
ORF length:
2979
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgccggcg ttggcgcgcg acggcggcca gctgccgctg ctcgttgttt
121tttctgcaat gatatttggg actattacaa atcaagatct gcctgtgatc aagtgtgttt
181taatcaatca taagaacaat gattcatcag tggggaagtc atcatcatat cccatggtat
241cagaatcccc ggaagacctc gggtgtgcgt tgagacccca gagctcaggg acagtgtacg
301aagctgccgc tgtggaagtg gatgtatctg cttccatcac actgcaagtg ctggtcgatg
361ccccagggaa catttcctgt ctctgggtct ttaagcacag ctccctgaat tgccagccac
421attttgattt acaaaacaga ggagttgttt ccatggtcat tttgaaaatg acagaaaccc
481aagctggaga atacctactt tttattcaga gtgaagctac caattacaca atattgttta
541cagtgagtat aagaaatacc ctgctttaca cattaagaag accttacttt agaaaaatgg
601aaaaccagga cgccctggtc tgcatatctg agagcgttcc agagccgatc gtggaatggg
661tgctttgcga ttcacagggg gaaagctgta aagaagaaag tccagctgtt gttaaaaagg
721aggaaaaagt gcttcatgaa ttatttggga cggacataag gtgctgtgcc agaaatgaac
781tgggcaggga atgcaccagg ctgttcacaa tagatctaaa tcaaactcct cagaccacat
841tgccacaatt atttcttaaa gtaggggaac ccttatggat aaggtgcaaa gctgttcatg
901tgaaccatgg attcgggctc acctgggaat tagaaaacaa agcactcgag gagggcaact
961actttgagat gagtacctat tcaacaaaca gaactatgat acggattctg tttgcttttg
1021tatcatcagt ggcaagaaac gacaccggat actacacttg ttcctcttca aagcatccca
1081gtcaatcagc tttggttacc atcgtagaaa agggatttat aaatgctacc aattcaagtg
1141aagattatga aattgaccaa tatgaagagt tttgtttttc tgtcaggttt aaagcctacc
1201cacaaatcag atgtacgtgg accttctctc gaaaatcatt tccttgtgag caaaagggtc
1261ttgataacgg atacagcata tccaagtttt gcaatcataa gcaccagcca ggagaatata
1321tattccatgc agaaaatgat gatgcccaat ttaccaaaat gttcacgctg aatataagaa
1381ggaaacctca agtgctcgca gaagcatcgg caagtcaggc gtcctgtttc tcggatggat
1441acccattacc atcttggacc tggaagaagt gttcagacaa gtctcccaac tgcacagaag
1501agatcacaga aggagtctgg aatagaaagg ctaacagaaa agtgtttgga cagtgggtgt
1561cgagcagtac tctaaacatg agtgaagcca taaaagggtt cctggtcaag tgctgtgcat
1621acaattccct tggcacatct tgtgagacga tccttttaaa ctctccaggc cccttccctt
1681tcatccaaga caacatctca ttctatgcaa caattggtgt ttgtctcctc ttcattgtcg
1741ttttaaccct gctaatttgt cacaagtaca aaaagcaatt taggtatgaa agccagctac
1801agatggtaca ggtgaccggc tcctcagata atgagtactt ctacgttgat ttcagagaat
1861atgaatatga tctcaaatgg gagtttccaa gagaaaattt agagtttggg aaggtactag
1921gatcaggtgc ttttggaaaa gtgatgaacg caacagctta tggaattagc aaaacaggag
1981tctcaatcca ggttgccgtc aaaatgctga aagaaaaagc agacagctct gaaagagagg
2041cactcatgtc agaactcaag atgatgaccc agctgggaag ccacgagaat attgtgaacc
2101tgctgggggc gtgcacactg tcaggaccaa tttacttgat ttttgaatac tgttgctatg
2161gtgatcttct caactatcta agaagtaaaa gagaaaaatt tcacaggact tggacagaga
2221ttttcaagga acacaatttc agtttttacc ccactttcca atcacatcca aattccagca
2281tgcctggttc aagagaagtt cagatacacc cggactcgga tcaaatctca gggcttcatg
2341ggaattcatt tcactctgaa gatgaaattg aatatgaaaa ccaaaaaagg ctggaagaag
2401aggaggactt gaatgtgctt acatttgaag atcttctttg ctttgcatat caagttgcca
2461aaggaatgga atttctggaa tttaagtcgt gtgttcacag agacctggcc gccaggaacg
2521tgcttgtcac ccacgggaaa gtggtgaaga tatgtgactt tggattggct cgacatatca
2581tgagtgattc caactatgtt gtcaggggca atgcccgtct gcctgtaaaa tggatggccc
2641ccgaaagcct gtttgaaggc atctacacca ttaagagtga tgtctggtca tatggaatat
2701tactgtggga aatcttctca cttggtgtga atccttaccc tggcattccg gttGATGCTA
2761ACTTCTACAA ACTGATTCAA AATGGATTTA AAATGGATCA GCCATTTTAT GCTACAGAAG
2821AAATATACAT TATAATGCAA TCCTGCTGGG CTTTTGACTC AAGGAAACGG CCATCCTTCC
2881CTAATTTGAC TTCGTTTTTA GGATGTCAGC TGGCAGATGC AGAAGAAGCG ATGTATCAGA
2941ATGTGGATGG CCGTGTTTCG GAATGTCCTC ACACCTACCA AAACAGGCGA CCTTTCAGCA
3001GAGAGATGGA TTTGGGGCTA CTCTCTCCGC AGGCTCAGGT CGAAGATTCG TAGGACCCAG
3061CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
3121ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
3181TATCTTGTGG AAAGGACGAT CCTTGCACTA ACCTTCTTCT CTAACGCGTT AAGTCgacaa
3241tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)

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