Construct: ORF TRCN0000488346
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021219.1_s317c1
- DNA Barcode:
- AGCCCAGACCAAATCGGACTCCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TRHR (7201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488346
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7201 | TRHR | thyrotropin releasing hormo... | NM_003301.6 | 100% | 100% | |
| 2 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | XM_006520747.1 | 90.2% | 94.4% | (many diffs) |
| 3 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | NM_013696.2 | 89.2% | 93.2% | (many diffs) |
| 4 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | XM_006520748.2 | 59.7% | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1266
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaaaac gagacagtca gtgaactgaa ccaaacacag cttcagccac 121 gagcagtggt ggccttagaa taccaggtgg tcaccatctt acttgtactc attatttgtg 181 gcctgggcat tgtaggcaac atcatggtag tcctggttgt catgagaacc aagcacatga 241 ggacccccac aaactgctac ctggtgagcc tggcagtagc tgatctcatg gtcttggtgg 301 ccgcaggcct ccccaacata acagacagta tctacggttc ctgggtctat ggctatgttg 361 gatgcctctg cattacttac ctccagtatt tgggaattaa tgcatcctct tgttcaataa 421 cagcctttac cattgagagg tacatagcaa tctgtcaccc catcaaagcc cagtttctct 481 gcacattttc cagagccaaa aagattatca tctttgtctg ggctttcaca tctctttact 541 gtatgctctg gttcttcttg ctggatctca atattagcac ctacaaagat gctattgtga 601 tatcctgtgg ctacaagatc tccaggaatt actactcacc tatttaccta atggactttg 661 gtgtctttta tgttgtgcca atgatcctgg ctaccgtcct ctatggattc atagctagaa 721 tccttttctt aaatcccatt ccttcagatc ctaaagaaaa ctctaagaca tggaaaaatg 781 attcaaccca tcagaacaca aatctgaatg taaatacctc taatagatgt ttcaacagca 841 cagtatcttc aaggaagcag gtcaccaaga tgctggcagt ggttgtaatt ctgtttgccc 901 ttttatggat gccctacagg actctagtgg ttgtcaactc atttctctcc agtcctTTCC 961 AAGAAAATTG GTTTTTGCTC TTTTGCAGAA TTTGCATTTA TCTCAACAGT GCCATCAACC 1021 CGGTGATTTA CAATCTCATG TCCCAGAAAT TCCGTGCAGC CTTCAGAAAG CTCTGCAACT 1081 GCAAGCAGAA GCCAACAGAG AAACCTGCTA ACTACAGTGT GGCCCTAAAT TACAGCGTCA 1141 TCAAGGAGTC AGACCATTTC AGCACAGAGC TTGATGATAT CACTGTCACT GACACTTACC 1201 TGTCTGCCAC AAAAGTGTCT TTTGATGACA CCTGCTTGGC TTCTGAGGTA TCCTTTAGCC 1261 AAAGTTGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCCCA GACCAAATCG GACTCCGCAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt