Construct: ORF TRCN0000488353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020701.1_s317c1
- DNA Barcode:
- GCTTAAACCCCCTGTGTGAACCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K7 (5609)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488353
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5609 | MAP2K7 | mitogen-activated protein k... | NM_001297556.2 | 99.8% | 100% | 51G>A;573C>T |
| 2 | human | 5609 | MAP2K7 | mitogen-activated protein k... | NM_145185.4 | 98.2% | 98.3% | 51G>A;573C>T;935_936ins21 |
| 3 | human | 5609 | MAP2K7 | mitogen-activated protein k... | XM_006722800.2 | 96.2% | 96.1% | 51G>A;125_172del;621C>T |
| 4 | human | 5609 | MAP2K7 | mitogen-activated protein k... | NM_001297555.2 | 94.6% | 94.5% | (many diffs) |
| 5 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_011944.3 | 88.7% | 97.1% | (many diffs) |
| 6 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001164172.1 | 85.5% | 93.4% | (many diffs) |
| 7 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001291777.1 | 82.2% | 89.9% | (many diffs) |
| 8 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001042557.2 | 79.5% | 86.7% | (many diffs) |
| 9 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001291783.1 | 72.7% | 80.2% | (many diffs) |
| 10 | mouse | 26400 | Map2k7 | mitogen-activated protein k... | NM_001291778.1 | 67.4% | 74.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1350
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggcg tcctccctgg aacagaagct gtcccgcctg gaagcaaagc 121 taaagcagga gaaccgggag gcccggcgga ggatcgacct caacctggat atcagccccc 181 agcggcccag gcccaccctg cagctcccgc tggccaacga tgggggcagc cgctcgccat 241 cctcagagag ctccccgcag caccccacgc cccccgcccg gccccgccac atgctggggc 301 tcccgtcaac cctgttcaca ccccgcagca tggagagcat tgagattgac cagaagctgc 361 aggagatcat gaagcagacg ggctacctga ccatcggggg ccagcgctac caggcagaaa 421 tcaacgacct ggagaacttg ggcgagatgg gcagcggcac ctgcggccag gtgtggaaga 481 tgcgcttccg gaagaccggc cacgtcattg ccgttaagca aatgcggcgc tccgggaaca 541 aggaggagaa caagcgcatc ctcatggacc tggatgtggt gctgaagagc cacgactgcc 601 cctacatcgt gcagtgcttt gggacgttca tcaccaacac ggatgtcttc atcgccatgg 661 agctcatggg cacctgcgct gagaagctca agaagcggat gcagggcccc atccccgagc 721 gcattctggg caagatgaca gtggcgattg tgaaggcgct gtactacctg aaggagaagc 781 acggtgtcat ccaccgcgac gtcaagccct ccaacatcct gctggacgag cggggccaga 841 tcaagctctg cgacttcggc atcagcggcc gcctggtgga ctccaaagcc aagacgcgga 901 gcgccggctg tgccgcctac atggcacccg agcgcattga ccccccagac cccaccaagc 961 cggactatga catccgggcc gacgtatgga gcctgggcat ctcgttgccc tgcccgtctc 1021 ccTCCCAGGT GGAGCTGGCA ACAGGACAGT TTCCCTACAA GAACTGCAAG ACGGACTTTG 1081 AGGTCCTCAC CAAAGTCCTA CAGGAAGAGC CCCCGCTTCT GCCCGGACAC ATGGGCTTCT 1141 CGGGGGACTT CCAGTCCTTC GTCAAAGACT GCCTTACTAA AGATCACAGG AAGAGACCAA 1201 AGTATAATAA GCTACTTGAA CACAGCTTCA TCAAGCGCTA CGAGACGCTG GAGGTGGACG 1261 TGGCGTCCTG GTTCAAGGAT GTCATGGCGA AGACTGAGTC ACCGCGGACT AGCGGCGTCC 1321 TGAGCCAGCC CCACCTGCCC TTCTTCAGGT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 1501 TTAAACCCCC TGTGTGAACC ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt