Construct: ORF TRCN0000488397
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021594.1_s317c1
- DNA Barcode:
- AATTGGCTATTAGTACGTGATGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CYB5A (1528)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488397
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1528 | CYB5A | cytochrome b5 type A | NM_148923.4 | 100% | 100% | |
| 2 | human | 1528 | CYB5A | cytochrome b5 type A | NM_001190807.3 | 92.5% | 92.5% | 258_259ins30 |
| 3 | human | 1528 | CYB5A | cytochrome b5 type A | NM_001914.4 | 72.8% | 72.3% | 292C>A;294_295ins108 |
| 4 | human | 1528 | CYB5A | cytochrome b5 type A | XM_011525835.2 | 72.8% | 72.3% | 292C>A;294_295ins108 |
| 5 | mouse | 109672 | Cyb5a | cytochrome b5 type A (micro... | NM_025797.4 | 86.3% | 87.3% | (many diffs) |
| 6 | mouse | 109672 | Cyb5a | cytochrome b5 type A (micro... | NM_001348159.1 | 63.1% | 63.4% | (many diffs) |
| 7 | mouse | 109672 | Cyb5a | cytochrome b5 type A (micro... | XM_017317803.1 | 63.1% | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 471
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcagagcag tcggacgagg ccgtgaagta ctacacccta gaggagattc 121 agaagcacaa ccacagcaag agcacctggc tgatcctgca ccacaaggtg tacgatttga 181 ccaaatttct GGAAGAGCAT CCTGGTGGGG AAGAAGTTTT AAGGGAACAA GCTGGAGGTG 241 ACGCTACTGA GAACTTTGAG GATGTCGGGC ACTCTACAGA TGCCAGGGAA ATGTCCAAAA 301 CATTCATCAT TGGGGAGCTC CATCCAGATG ACAGACCAAA GTTAAACAAG CCTCCGGAAA 361 CTCTTATCAC TACTATTGAT TCTAGTTCCA GTTGGTGGAC CAACTGGGTG ATCCCTGCCA 421 TCTCTGCAGT GGCCGTCGCC TTGATGTATC GCCTATACAT GGCAGAGGAC TAGAACCCAG 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAA ATTGGCTATT AGTACGTGAT GGTACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att