Construct: ORF TRCN0000488407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020630.1_s317c1
- DNA Barcode:
- TGCGAACGCTCCCGGTACCAGGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ZAP70 (7535)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488407
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7535 | ZAP70 | zeta chain of T cell recept... | NM_001079.3 | 99.9% | 100% | 1677G>A |
| 2 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004867.1 | 83.3% | 83.4% | 1_369del;2046G>A |
| 3 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004868.1 | 82.5% | 82.6% | 1_369del;1254_1255insCCAGCACGCATAACGTCC;2028G>A |
| 4 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004869.1 | 79.2% | 78.5% | (many diffs) |
| 5 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004870.1 | 78.5% | 77.8% | (many diffs) |
| 6 | human | 7535 | ZAP70 | zeta chain of T cell recept... | NM_207519.1 | 50.3% | 50.4% | 0_1ins921;756G>A |
| 7 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738927.1 | 49.5% | (many diffs) | |
| 8 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738926.1 | 42.2% | (many diffs) | |
| 9 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738925.1 | 37.6% | (many diffs) | |
| 10 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289765.1 | 87.6% | 93.5% | (many diffs) |
| 11 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_009539.3 | 87.6% | 93.5% | (many diffs) |
| 12 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | XM_006495896.2 | 87.6% | 93.5% | (many diffs) |
| 13 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289766.1 | 84.3% | 90% | (many diffs) |
| 14 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289612.1 | 44.1% | 47% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1929
- ORF length:
- 1857
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccagac cccgcggcgc acctgccctt cttctacggc agcatctcgc 121 gtgccgaggc cgaggagcac ctgaagctgg cgggcatggc ggacgggctc ttcctgctgc 181 gccagtgcct gcgctcgctg ggcggctatg tgctgtcgct cgtgcacgat gtgcgcttcc 241 accactttcc catcgagcgc cagctcaacg gcacctacgc cattgccggc ggcaaagcgc 301 actgtggacc ggcagagctc tgcgagttct actcgcgcga ccccgacggg ctgccctgca 361 acctgcgcaa gccgtgcaac cggccgtcgg gcctcgagcc gcagccgggg gtcttcgact 421 gcctgcgaga cgccatggtg cgtgactacg tgcgccagac gtggaagctg gagggcgagg 481 ccctggagca ggccatcatc agccaggccc cgcaggtgga gaagctcatt gctacgacgg 541 cccacgagcg gatgccctgg taccacagca gcctgacgcg tgaggaggcc gagcgcaaac 601 tttactctgg ggcgcagacc gacggcaagt tcctgctgag gccgcggaag gagcagggca 661 catacgccct gtccctcatc tatgggaaga cggtgtacca ctacctcatc agccaagaca 721 aggcgggcaa gtactgcatt cccgagggca ccaagtttga cacgctctgg cagctggtgg 781 agtatctgaa gctgaaggcg gacgggctca tctactgcct gaaggaggcc tgccccaaca 841 gcagtgccag caacgcctca ggggctgctg ctcccacact cccagcccac ccatccacgt 901 tgactcatcc tcagagacga atcgacaccc tcaactcaga tggatacacc cctgagccag 961 cacgcataac gtccccagac aaaccgcggc cgatgcccat ggacacgagc gtgtatgaga 1021 gcccctacag cgacccagag gagctcaagg acaagaagct cttcctgaag cgcgataacc 1081 tcctcatagc tgacattgaa cttggctgcg gcaactttgg ctcagtgcgc cagggcgtgt 1141 accgcatgcg caagaagcag atcgacgtgg ccatcaaggt gctgaagcag ggcacggaga 1201 aggcagacac ggaagagatg atgcgcgagg cgcagatcat gcaccagctg gacaacccct 1261 acatcgtgcg gctcattggc gtctgccagg ccgaggccct catgctggtc atggagatgg 1321 ctgggggcgg gccgctgcac aagttcctgg tcggcaagag ggaggagatc cctgtgagca 1381 atgtggccga gctgctgcac caggtgtcca tggggatgaa gtacctggag gagaagaact 1441 ttgtgcaccg tgacctggcg gcccgcaacg tcctgctggt taaccggcac tacgccaaga 1501 tcagcgactt tggcctctcc aaagcactgg gtgccgacga cagctactac actgcccgct 1561 cagcagggaa gtggccgctc aagtggtacg cacccgaatg catcaacttc cgcaagttct 1621 ccagccgcag cgatgtctgg agctatgggg tcaccatgtg ggaggccttg tcctacggcc 1681 agaagcccTA CAAGAAGATG AAAGGGCCGG AGGTCATGGC CTTCATCGAG CAGGGCAAGC 1741 GGATGGAATG CCCACCAGAG TGTCCACCCG AACTGTACGC ACTCATGAGT GACTGCTGGA 1801 TCTACAAGTG GGAGGATCGC CCCGACTTCC TGACCGTGGA GCAGCGCATG CGAGCCTGTT 1861 ACTACAGCCT GGCCAGCAAG GTGGAAGGGC CCCCAGGCAG CACACAGAAG GCTGAGGCTG 1921 CCTGTGCCTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1981 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2041 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGC GAACGCTCCC GGTACCAGGA 2101 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t