Construct: ORF TRCN0000488421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019827.1_s317c1
- DNA Barcode:
- CTTACATAACTAACCGCCTCGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRM7 (2917)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488421
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2917 | GRM7 | glutamate metabotropic rece... | NM_000844.4 | 99.9% | 99.8% | 2745_2746insG |
| 2 | human | 2917 | GRM7 | glutamate metabotropic rece... | NM_181874.2 | 98.4% | 97.9% | (many diffs) |
| 3 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_017006273.1 | 81.5% | 81.1% | (many diffs) |
| 4 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_017006272.1 | 80.2% | 79.2% | (many diffs) |
| 5 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453492.1 | 53.2% | 52.7% | (many diffs) |
| 6 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453493.1 | 53.2% | 52.7% | (many diffs) |
| 7 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453494.1 | 53.2% | 52.7% | (many diffs) |
| 8 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453495.1 | 53.2% | 52.7% | (many diffs) |
| 9 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453497.1 | 53.2% | 52.7% | (many diffs) |
| 10 | human | 2917 | GRM7 | glutamate metabotropic rece... | XM_024453498.1 | 53.2% | 52.7% | (many diffs) |
| 11 | human | 2917 | GRM7 | glutamate metabotropic rece... | XR_001740136.2 | 48.4% | 1_274del;2724_2747del;3044_5671delinsG | |
| 12 | human | 2917 | GRM7 | glutamate metabotropic rece... | XR_001740135.2 | 25.3% | (many diffs) | |
| 13 | human | 2917 | GRM7 | glutamate metabotropic rece... | XR_001740134.2 | 24.9% | (many diffs) | |
| 14 | human | 2917 | GRM7 | glutamate metabotropic rece... | XR_001740137.2 | 24.9% | (many diffs) | |
| 15 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | NM_177328.3 | 91.6% | 99.3% | (many diffs) |
| 16 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505301.1 | 90.5% | 96.9% | (many diffs) |
| 17 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | NM_001346640.1 | 90.1% | 97.3% | (many diffs) |
| 18 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505298.2 | 90.1% | 97.3% | (many diffs) |
| 19 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505299.3 | 90.1% | 97.3% | (many diffs) |
| 20 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505300.2 | 90.1% | 96.5% | (many diffs) |
| 21 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505296.1 | 90% | 96.4% | (many diffs) |
| 22 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_017321298.1 | 90% | 96.4% | (many diffs) |
| 23 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XM_006505302.2 | 81.8% | 88.6% | (many diffs) |
| 24 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XR_868843.1 | 75.1% | (many diffs) | |
| 25 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XR_001785079.1 | 59.7% | (many diffs) | |
| 26 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XR_001785080.1 | 59.4% | (many diffs) | |
| 27 | mouse | 108073 | Grm7 | glutamate receptor, metabot... | XR_001785078.1 | 58.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2817
- ORF length:
- 2748
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtccagctg aggaagctgc tccgcgtcct gactttgatg aagttcccct 121 gctgcgtgct ggaggtgctc ctgtgcgcgc tggcggcggc ggcgcgcggc caggagatgt 181 acgccccgca ctcaatccgg atcgaggggg acgtcaccct cggggggctg ttccccgtgc 241 acgccaaggg tcccagcgga gtgccctgcg gcgacatcaa gagggaaaac gggatccaca 301 ggctggaagc gatgctctac gccctggacc agatcaacag tgatcccaac ctactgccca 361 acgtgacgct gggcgcgcgg atcctggaca cttgttccag ggacacttac gcgctcgaac 421 agtcgcttac tttcgtccag gcgctcatcc agaaggacac ctccgacgtg cgctgcacca 481 acggcgaacc gccggttttc gtcaagccgg agaaagtagt tggagtgatt ggggcttcgg 541 ggagttcggt ctccatcatg gtagccaaca tcctgaggct cttccagatc ccccagatta 601 gttatgcatc aacggcaccc gagctaagtg atgaccggcg ctatgacttc ttctctcgcg 661 tggtgccacc cgattccttc caagcccagg ccatggtaga cattgtaaag gccctaggct 721 ggaattatgt gtctaccctc gcatcggaag gaagttatgg agagaaaggt gtggagtcct 781 tcacgcagat ttccaaagag gcaggtggac tctgcattgc ccagtccgtg agaatccccc 841 aggaacgcaa agacaggacc attgactttg atagaattat caaacagctc ctggacaccc 901 ccaactccag ggccgtcgtg atttttgcca acgatgagga tataaagcag atccttgcag 961 cagccaaaag agctgaccaa gttggccatt ttctttgggt gggatcagac agctggggat 1021 ccaaaataaa cccactgcac cagcatgaag atatcgcaga aggggccatc accattcagc 1081 ccaagcgagc cacggtggaa gggtttgatg cctactttac gtcccgtaca cttgaaaaca 1141 acagaagaaa tgtatggttt gccgaatact gggaggaaaa cttcaactgc aagttgacga 1201 ttagtgggtc aaaaaaagaa gacacagatc gcaaatgcac aggacaggag agaattggaa 1261 aagattccaa ctatgagcag gagggtaaag tccagttcgt gattgacgca gtctatgcta 1321 tggctcacgc ccttcaccac atgaacaagg atctctgtgc tgactaccgg ggtgtctgcc 1381 cagagatgga gcaagctgga ggcaagaagt tgctgaagta tatacgcaat gttaatttca 1441 atggtagtgc tggcactcca gtgatgttta acaagaacgg ggatgcacct gggcgttatg 1501 acatctttca gtaccagacc acaaacacca gcaacccggg ttaccgtctg atcgggcagt 1561 ggacagacga acttcagctc aatatagaag acatgcagtg gggtaaagga gtccgagaga 1621 tacccgcctc agtgtgcaca ctaccatgta agccaggaca gagaaagaag acacagaaag 1681 gaactccttg ctgttggacc tgtgagcctt gcgatggtta ccagtaccag tttgatgaga 1741 tgacatgcca gcattgcccc tatgaccaga ggcccaatga aaatcgaacc ggatgccagg 1801 atattcccat catcaaactg gagtggcact ccccctgggc tgtgattcct gtcttcctgg 1861 caatgttggg gatcattgcc accatctttg tcatggccac tttcatccgc tacaatgaca 1921 cgcccattgt ccgggcatct gggcgggaac tcagctatgt tcttttgacg ggcatctttc 1981 tttgctacat catcactttc ctgatgattg ccaaaccaga tgtggcagtg tgttctttcc 2041 ggcgagtttt cttgggcttg ggtatgtgca tcagttatgc agccctcttg acgaaaacaa 2101 atcggattta tcgcatattt gagcagggca agaaatcagt aacagctccc agactcataa 2161 gcccaacatc acaactggca atcacttcca gtttaatatc agttcagctt ctaggggtgt 2221 tcatttggtt tggtgttgat ccacccaaca tcatcataga ctatgatgaa cacaagacaa 2281 tgaaccctga gcaagccaga ggggttctca agtgtgacat tacagatctc caaatcattt 2341 gctccttggg atatagcatt cttctcatgg tcacatgtac tgtgtatgcc atcaagactc 2401 ggggtgtacc cgagaatttt aacgaagcca agcccattgg attcactatg tacacgacat 2461 gtatagtatg gcttgccttc attccaattt tttttGGCAC CGCTCAATCA GCGGAAAAGC 2521 TCTACATACA AACTACCACG CTTACAATCT CCATGAACCT AAGTGCATCA GTGGCGCTGG 2581 GGATGCTATA CATGCCGAAA GTGTACATCA TCATTTTCCA CCCTGAACTC AATGTCCAGA 2641 AACGGAAGCG AAGCTTCAAG GCGGTAGTCA CAGCAGCCAC CATGTCATCG AGGCTGTCAC 2701 ACAAACCCAG TGACAGACCC AACGGTGAGG CAAAGACCGA GCTCTGTGAA AACGTAGACC 2761 CAAACAGCCC TGCTGCAAAA AAGAAGTATG TCAGTTATAA TAACCTGGTT ATCGACCCAG 2821 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2881 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2941 TATCTTGTGG AAAGGACGAC TTACATAACT AACCGCCTCG ATAACGCGTT AAGTCgacaa 3001 tcaacctctg gattacaaaa tttgtgaaag att