Construct: ORF TRCN0000488448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021861.1_s317c1
- DNA Barcode:
- GACGTTCAGTGGAATCGCTATCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NR1D2 (9975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488448
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NM_005126.5 | 99.9% | 100% | 1179T>C |
| 2 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NM_001145425.1 | 86.9% | 87% | 0_1ins225;954T>C |
| 3 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | XM_006713451.3 | 77.2% | 76.6% | (many diffs) |
| 4 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NR_110524.2 | 32.5% | (many diffs) | |
| 5 | mouse | 353187 | Nr1d2 | nuclear receptor subfamily ... | NM_011584.4 | 88.6% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1806
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaggtgaat gcaggaggtg tgattgccta tatcagttct tccagctcag 121 cctcaagccc tgcctcttgt cacagtgagg gttctgagaa tagtttccag tcctcctcct 181 cttctgttcc atcttctcca aatagctcta attctgatac caatggtaat cccaagaatg 241 gtgatctcgc caatattgaa ggcatcttga agaatgatcg aatagattgt tctatgaaaa 301 caagcaaatc gagtgcacct gggatgacaa aaagtcatag tggtgtgaca aaatttagtg 361 gcatggttct actgtgtaaa gtctgtgggg atgtggcgtc aggattccac tatggagttc 421 atgcttgcga aggctgtaag ggtttctttc ggagaagtat tcaacaaaac atccagtaca 481 agaagtgcct gaagaatgaa aactgttcta taatgagaat gaataggaac agatgtcagc 541 aatgtcgctt caaaaagtgt ctgtctgttg gaatgtcaag agatgctgtt cggtttggtc 601 gtattcctaa gcgtgaaaaa cagaggatgc taattgaaat gcaaagtgca atgaagacca 661 tgatgaacag ccagttcagt ggtcacttgc aaaatgacac attagtagaa catcatgaac 721 agacagcctt gccagcccag gaacagctgc gacccaagcc ccaactggag caagaaaaca 781 tcaaaagctc ttctcctcca tcttctgatt ttgcaaagga agaagtgatt ggcatggtga 841 ccagagctca caaggatacc tttatgtata atcaagagca gcaagaaaac tcagctgaga 901 gcatgcagcc ccagagagga gaacggattc ccaagaacat ggagcaatat aatttaaatc 961 atgatcattg cggcaatggg cttagcagcc attttccctg tagtgagagc cagcagcatc 1021 tcaatggaca gttcaaaggg aggaatataa tgcattaccc aaatggtcat gccatttgta 1081 ttgcaaatgg acattgtatg aacttctcca atgcttatac tcaaagagta tgtgatagag 1141 ttccgataga tggattttct cagaatgaga acaagaatag ttacctgtgc aacactggag 1201 gaagaatgca tctggtttgt ccaatgagta agtctccata tgtggaccct cataaatcag 1261 gacatgaaat ctgggaagaa ttttcgatga gcttcactcc agcagtgaaa gaagtggtgg 1321 aatttgcaaa gcgtattcct gggttcagag atctctctca gcatgaccag gtcaaccttt 1381 taaaggctgg gacttttgag gttttaatgg tacggttcgc atcattattt gatgcaaagg 1441 aacgtactgt caccttttta agtggaaaga aatatagtgt ggatgattta cactcaatGG 1501 GAGCAGGGGA TCTGCTAAAC TCTATGTTTG AATTTAGTGA GAAGCTAAAT GCCCTCCAAC 1561 TTAGTGATGA AGAGATGAGT TTGTTTACAG CTGTTGTCCT GGTATCTGCA GATCGATCTG 1621 GAATAGAAAA CGTCAACTCT GTGGAGGCTT TGCAGGAAAC TCTCATTCGT GCACTAAGGA 1681 CCTTAATAAT GAAAAACCAT CCAAATGAGG CCTCTATTTT TACAAAACTG CTTCTAAAGT 1741 TGCCAGATCT TCGATCTTTA AACAACATGC ACTCTGAGGA GCTCTTGGCC TTTAAAGTTC 1801 ACCCTTAAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1861 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1921 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACGTT CAGTGGAATC GCTATCCCAC 1981 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt