Construct: ORF TRCN0000488471

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021952.1_s317c1
DNA Barcode:
GAGTTATTTCACGGACACGACAAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MET (4233)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488471
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4233 MET MET proto-oncogene, recepto... NM_000245.4 99.9% 99.9% 2121T>C;2124A>G;3703T>G
2 human 4233 MET MET proto-oncogene, recepto... NM_001127500.3 98.6% 98.6% (many diffs)
3 human 4233 MET MET proto-oncogene, recepto... XM_011516223.1 98.5% 98.5% (many diffs)
4 human 4233 MET MET proto-oncogene, recepto... NM_001324402.2 68.9% 68.9% (many diffs)
5 human 4233 MET MET proto-oncogene, recepto... XM_006715990.2 68.9% 68.9% (many diffs)
6 human 4233 MET MET proto-oncogene, recepto... NM_001324401.2 66.6% 65.9% (many diffs)
7 human 4233 MET MET proto-oncogene, recepto... XR_001744772.1 61% (many diffs)
8 mouse 17295 Met met proto-oncogene NM_008591.2 85.7% 88.8% (many diffs)
9 mouse 17295 Met met proto-oncogene XM_011241042.2 85.7% 88.8% (many diffs)
10 mouse 17295 Met met proto-oncogene XM_011241043.2 85.7% 88.8% (many diffs)
11 mouse 17295 Met met proto-oncogene XM_006505002.3 82.4% 85.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
4242
ORF length:
4170
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaaggcc cccgctgtgc ttgcacctgg catcctcgtg ctcctgttta
121ccttggtgca gaggagcaat ggggagtgta aagaggcact agcaaagtcc gagatgaatg
181tgaatatgaa gtatcagctt cccaacttca ccgcggaaac acccatccag aatgtcattc
241tacatgagca tcacattttc cttggtgcca ctaactacat ttatgtttta aatgaggaag
301accttcagaa ggttgctgag tacaagactg ggcctgtgct ggaacaccca gattgtttcc
361catgtcagga ctgcagcagc aaagccaatt tatcaggagg tgtttggaaa gataacatca
421acatggctct agttgtcgac acctactatg atgatcaact cattagctgt ggcagcgtca
481acagagggac ctgccagcga catgtctttc cccacaatca tactgctgac atacagtcgg
541aggttcactg catattctcc ccacagatag aagagcccag ccagtgtcct gactgtgtgg
601tgagcgccct gggagccaaa gtcctttcat ctgtaaagga ccggttcatc aacttctttg
661taggcaatac cataaattct tcttatttcc cagatcatcc attgcattcg atatcagtga
721gaaggctaaa ggaaacgaaa gatggtttta tgtttttgac ggaccagtcc tacattgatg
781ttttacctga gttcagagat tcttacccca ttaagtatgt ccatgccttt gaaagcaaca
841attttattta cttcttgacg gtccaaaggg aaactctaga tgctcagact tttcacacaa
901gaataatcag gttctgttcc ataaactctg gattgcattc ctacatggaa atgcctctgg
961agtgtattct cacagaaaag agaaaaaaga gatccacaaa gaaggaagtg tttaatatac
1021ttcaggctgc gtatgtcagc aagcctgggg cccagcttgc tagacaaata ggagccagcc
1081tgaatgatga cattcttttc ggggtgttcg cacaaagcaa gccagattct gccgaaccaa
1141tggatcgatc tgccatgtgt gcattcccta tcaaatatgt caacgacttc ttcaacaaga
1201tcgtcaacaa aaacaatgtg agatgtctcc agcattttta cggacccaat catgagcact
1261gctttaatag gacacttctg agaaattcat caggctgtga agcgcgccgt gatgaatatc
1321gaacagagtt taccacagct ttgcagcgcg ttgacttatt catgggtcaa ttcagcgaag
1381tcctcttaac atctatatcc accttcatta aaggagacct caccatagct aatcttggga
1441catcagaggg tcgcttcatg caggttgtgg tttctcgatc aggaccatca acccctcatg
1501tgaattttct cctggactcc catccagtgt ctccagaagt gattgtggag catacattaa
1561accaaaatgg ctacacactg gttatcactg ggaagaagat cacgaagatc ccattgaatg
1621gcttgggctg cagacatttc cagtcctgca gtcaatgcct ctctgcccca ccctttgttc
1681agtgtggctg gtgccacgac aaatgtgtgc gatcggagga atgcctgagc gggacatgga
1741ctcaacagat ctgtctgcct gcaatctaca aggttttccc aaatagtgca ccccttgaag
1801gagggacaag gctgaccata tgtggctggg actttggatt tcggaggaat aataaatttg
1861atttaaagaa aactagagtt ctccttggaa atgagagctg caccttgact ttaagtgaga
1921gcacgatgaa tacattgaaa tgcacagttg gtcctgccat gaataagcat ttcaatatgt
1981ccataattat ttcaaatggc cacgggacaa cacaatacag tacattctcc tatgtggatc
2041ctgtaataac aagtatttcg ccgaaatacg gtcctatggc tggtggcact ttacttactt
2101taactggaaa ttacctaaac agtgggaatt ctagacacat ttcaattggt ggaaaaacat
2161gtactttaaa aagtgtgtca aacagtattc tcgagtgtta taccccagcc caaaccattt
2221caactgagtt tgctgttaaa ttgaaaattg acttagccaa ccgagagaca agcatcttca
2281gttaccgtga agatcccatt gtctatgaaa ttcatccaac caaatctttt attagtggtg
2341ggagcacaat aacaggtgtt gggaaaaacc tgaattcagt tagtgtcccg agaatggtca
2401taaatgtgca tgaagcagga aggaacttta cagtggcatg tcaacatcgc tctaattcag
2461agataatctg ttgtaccact ccttccctgc aacagctgaa tctgcaactc cccctgaaaa
2521ccaaagcctt tttcatgtta gatgggatcc tttccaaata ctttgatctc atttatgtac
2581ataatcctgt gtttaagcct tttgaaaagc cagtgatgat ctcaatgggc aatgaaaatg
2641tactggaaat taagggaaat gatattgacc ctgaagcagt taaaggtgaa gtgttaaaag
2701ttggaaataa gagctgtgag aatatacact tacattctga agccgtttta tgcacggtcc
2761ccaatgacct gctgaaattg aacagcgagc taaatataga gtggaagcaa gcaatttctt
2821caaccgtcct tggaaaagta atagttcaac cagatcagaa tttcacagga ttgattgctg
2881gtgttgtctc aatatcaaca gcactgttat tactacttgg gtttttcctg tggctgaaaa
2941agagaaagca aattaaagat ctgggcagtg aattagttcg ctacgatgca agagtacaca
3001ctcctcattt ggataggctt gtaagtgccc gaagtgtaag cccaactaca gaaatggttt
3061caaatgaatc tgtagactac cgagctactt ttccagaaga tcagtttcct aattcatctc
3121agaacggttc atgccgacaa gtgcagtatc ctctgacaga catgtccccc atcctaacta
3181gtggggactc tgatatatcc agtccattac tgcaaaatac tgtccacatt gacctcagtg
3241ctctaaatcc agagctggtc caggcagtgc agcatgtagt gattgggccc agtagcctga
3301ttgtgcattt caatgaagtc ataggaagag ggcattttgg ttgtgtatat catgggactt
3361tgttggacaa tgatggcaag aaaattcact gtgctgtgaa atccttgaac agaatcactg
3421acataggaga agtttcccaa tttctgaccg agggaatcat catgaaagat tttagtcatc
3481ccaatgtcct ctcgctcctg ggaatctgcc tgcgaagtga agggtctccg ctggtggtcc
3541taccatacat gaaacatgga gatcttcgaa atttcattcg aaatgagact cataatccaa
3601ctgtaaaaga tcttattggc tttggtcttc aagtagccaa aggcatgaaa tatcttgcaa
3661gcaaaaagtt tgtccacaga gacttggctg caagaaactg tatgctggat gaaaaattca
3721cagtcaaggt tgctgatttt ggtcttgcca gagacatgta tgataaagaa tacgatagtg
3781tacacaacaa aacaggtgca aagctgccag tgaagtggat ggctttggaa agtctgcaaa
3841ctcaaaagtt taccaccaag tcagatgtgt ggtcctttgg cgtgctcctc tgggagctga
3901tgacaagagg agccccacct tatcctgacg taaacacctt tgatataact gtttacttgt
3961tgcaaggGAG AAGACTCCTA CAACCCGAAT ACTGCCCAGA CCCCTTATAT GAAGTAATGC
4021TAAAATGCTG GCACCCTAAA GCCGAAATGC GCCCATCCTT TTCTGAACTG GTGTCCCGGA
4081TATCAGCGAT CTTCTCTACT TTCATTGGGG AGCACTATGT CCATGTGAAC GCTACTTATG
4141TGAACGTAAA ATGTGTCGCT CCGTATCCTT CTCTGTTGTC ATCAGAAGAT AACGCTGATG
4201ATGAGGTGGA CACACGACCA GCCTCCTTCT GGGAGACATC ATAGGACCCA GCTTTCTTGT
4261ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
4321AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
4381GAAAGGACGA GAGTTATTTC ACGGACACGA CAACACGCGT TAAGTCgaca atcaacctct
4441ggattacaaa atttgtgaaa gatt

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