Transcript: Mouse XM_006505002.3

PREDICTED: Mus musculus met proto-oncogene (Met), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Met (17295)
Length:
6726
CDS:
571..4569

Additional Resources:

NCBI RefSeq record:
XM_006505002.3
NBCI Gene record:
Met (17295)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006505002.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000226124 GCATGGAGATCTGCGAAATTT pLKO_005 3906 CDS 100% 15.000 21.000 N Met n/a
2 TRCN0000226121 TCCTACACGGCCATCATATTT pLKO_005 737 CDS 100% 15.000 21.000 N Met n/a
3 TRCN0000218052 ATAAGTAACTTGCGGTGATAA pLKO_005 5552 3UTR 100% 13.200 18.480 N Met n/a
4 TRCN0000023530 GCACGACAAATACGTTGAAAT pLKO.1 2417 CDS 100% 13.200 18.480 N Met n/a
5 TRCN0000196414 GATAAGGAAATGTACTGATTG pLKO.1 6104 3UTR 100% 10.800 15.120 N MET n/a
6 TRCN0000023529 CGGGATTCTTTCCAAACACTT pLKO.1 3039 CDS 100% 4.950 6.930 N Met n/a
7 TRCN0000040046 CGAGCTAAATATAGAGTGGAA pLKO.1 3282 CDS 100% 2.640 3.696 N MET n/a
8 TRCN0000226122 TTGACAGACCAGTCCTATATT pLKO_005 1252 CDS 100% 15.000 10.500 N Met n/a
9 TRCN0000226123 TAAGCGAGAGCACGACAAATA pLKO_005 2408 CDS 100% 13.200 9.240 N Met n/a
10 TRCN0000023531 CCCGACGTGAACACATTTGAT pLKO.1 4276 CDS 100% 5.625 3.938 N Met n/a
11 TRCN0000023532 CCAGTCCTATATTGATGTCTT pLKO.1 1260 CDS 100% 4.950 3.465 N Met n/a
12 TRCN0000121089 AGACTCATAATCCAACTGTAA pLKO.1 3938 CDS 100% 4.950 3.465 N MET n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006505002.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14696 pDONR223 0% 82.5% 85.4% None (many diffs) n/a
2 ccsbBroad304_14696 pLX_304 15.1% 82.5% 85.4% V5 (many diffs) n/a
3 TRCN0000471392 ACGGTCGACCTTTCGGTGCTTCCT pLX_317 8.8% 82.5% 85.4% V5 (many diffs) n/a
4 TRCN0000492212 TGCCAACATGACGCCTACAAGGAA pLX_317 10.6% 82.4% 85.4% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489303 ACTAGCTCCGCGCACTTAACCCAA pLX_317 8.9% 82.4% 85.4% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000488821 CATCAAGAAACTATTACTCCGATA pLX_317 7.8% 82.4% 85.3% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000488471 GAGTTATTTCACGGACACGACAAC pLX_317 6.8% 82.4% 85.3% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000492123 CGGATCTTCCTACATAGGCCATCA pLX_317 28% 26.6% .1% V5 (not translated due to prior stop codon) (many diffs) n/a
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