Construct: ORF TRCN0000488485
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020405.1_s317c1
- DNA Barcode:
- GTTCCTGCCATCGTCTCGCAAGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ACYP1 (97)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488485
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001107.5 | 100% | 100% | |
| 2 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001302616.2 | 100% | 100% | |
| 3 | human | 97 | ACYP1 | acylphosphatase 1 | NM_001302617.2 | 76.7% | 76.7% | 1_90del |
| 4 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126393.2 | 45% | 1_79del;161_239del;456_659del | |
| 5 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126395.2 | 43.1% | (many diffs) | |
| 6 | human | 97 | ACYP1 | acylphosphatase 1 | NR_126394.2 | 37% | (many diffs) | |
| 7 | mouse | 66204 | Acyp1 | acylphosphatase 1, erythroc... | NM_025421.2 | 85.9% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 369
- ORF length:
- 297
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 CAGGCTTCAC CATGGCAGAA GGAAACACCC TGATATCAGT GGATTATGAA ATTTTTGGGA 121 AGGTGCAAGG GGTGTTTTTC CGTAAGCATA CTCAGGCTGA GGGTAAAAAG CTGGGATTGG 181 TAGGCTGGGT CCAGAACACT GACCGGGGCA CAGTGCAAGG ACAATTGCAA GGTCCCATCT 241 CCAAGGTGCG TCATATGCAG GAATGGCTTG AAACAAGAGG AAGTCCTAAA TCACACATCG 301 ACAAAGCAAA CTTCAACAAT GAAAAAGTCA TCTTGAAGTT GGATTACTCA GACTTCCAAA 361 TTGTAAAATA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 421 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 481 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT CCTGCCATCG TCTCGCAAGA 541 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t