Construct: ORF TRCN0000488535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020425.1_s317c1
- DNA Barcode:
- ATAGGCAGGATTGATTGGACAGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PAK1 (5058)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488535
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | NM_001128620.1 | 98.5% | 100% | 1659_1660ins25 |
| 2 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545080.3 | 98.5% | 100% | 1659_1660ins25 |
| 3 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545081.2 | 98.5% | 100% | 1659_1660ins25 |
| 4 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545082.2 | 98.5% | 100% | 1659_1660ins25 |
| 5 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545083.2 | 98.5% | 100% | 1659_1660ins25 |
| 6 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545084.3 | 98.5% | 100% | 1659_1660ins25 |
| 7 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448557.1 | 98.5% | 100% | 1659_1660ins25 |
| 8 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448558.1 | 98.5% | 100% | 1659_1660ins25 |
| 9 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448559.1 | 98.5% | 100% | 1659_1660ins25 |
| 10 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | NM_002576.4 | 97% | 94.4% | 1550_1551ins49 |
| 11 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017846.1 | 97% | 94.4% | 1550_1551ins49 |
| 12 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017847.2 | 97% | 94.4% | 1550_1551ins49 |
| 13 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017848.1 | 97% | 94.4% | 1550_1551ins49 |
| 14 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017849.1 | 97% | 94.4% | 1550_1551ins49 |
| 15 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017850.1 | 97% | 94.4% | 1550_1551ins49 |
| 16 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448560.1 | 97% | 94.4% | 1550_1551ins49 |
| 17 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448561.1 | 97% | 94.4% | 1550_1551ins49 |
| 18 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448562.1 | 97% | 94.4% | 1550_1551ins49 |
| 19 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448563.1 | 97% | 94.4% | 1550_1551ins49 |
| 20 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448564.1 | 97% | 94.4% | 1550_1551ins49 |
| 21 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | NM_011035.2 | 89.4% | 93.5% | (many diffs) |
| 22 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_017322028.1 | 89.4% | 93.5% | (many diffs) |
| 23 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507434.1 | 88.2% | 92.1% | (many diffs) |
| 24 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507436.3 | 88.2% | 92.1% | (many diffs) |
| 25 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507437.2 | 88.2% | 92.1% | (many diffs) |
| 26 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_011241694.1 | 88.2% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 1743
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgtcaa ataacggcct agacattcaa gacaaacccc 121 cagcccctcc gatgagaaat accagcacta tgattggagc cggcagcaaa gatgctggaa 181 ccctaaacca tggttctaaa cctctgcctc caaacccaga ggagaagaaa aagaaggacc 241 gattttaccg atccatttta cctggagata aaacaaataa aaagaaagag aaagagcggc 301 cagagatttc tctcccttca gattttgaac acacaattca tgtcggtttt gatgctgtca 361 caggggagtt tacgggaatg ccagagcagt gggcccgctt gcttcagaca tcaaatatca 421 ctaagtcgga gcagaagaaa aacccgcagg ctgttctgga tgtgttggag ttttacaact 481 cgaagaagac atccaacagc cagaaataca tgagctttac agataagtca gctgaggatt 541 acaattcttc taatgccttg aatgtgaagg ctgtgtctga gactcctgca gtgccaccag 601 tttcagaaga tgaggatgat gatgatgatg atgctacccc accaccagtg attgctccac 661 gcccagagca cacaaaatct gtatacacac ggtctgtgat tgaaccactt cctgtcactc 721 caactcggga cgtggctaca tctcccattt cacctactga aaataacacc actccaccag 781 atgctttgac ccggaatact gagaagcaga agaagaagcc taaaatgtct gatgaggaga 841 tcttggagaa attacgaagc atagtgagtg tgggcgatcc taagaagaaa tatacacggt 901 ttgagaagat tggacaaggt gcttcaggca ccgtgtacac agcaatggat gtggccacag 961 gacaggaggt ggccattaag cagatgaatc ttcagcagca gcccaagaaa gagctgatta 1021 ttaatgagat cctggtcatg agggaaaaca agaacccaaa cattgtgaat tacttggaca 1081 gttacctcgt gggagatgag ctgtgggttg ttatggaata cttggctgga ggctccttga 1141 cagatgtggt gacagaaact tgcatggatg aaggccaaat tgcagctgtg tgccgtgagt 1201 gtctgcaggc tctggagttc ttgcattcga accaggtcat tcacagagac atcaagagtg 1261 acaatattct gttgggaatg gatggctctg tcaagctaac tgactttgga ttctgtgcac 1321 agataacccc agagcagagc aaacggagca ccatggtagg aaccccatac tggatggcac 1381 cagaggttgt gacacgaaag gcctatgggc ccaaggttga catctggtcc ctgggcatca 1441 tggccatcga aatgattgaa ggggagcctc cataccTCAA TGAAAACCCT CTGAGAGCCT 1501 TGTACCTCAT TGCCACCAAT GGGACCCCAG AACTTCAGAA CCCAGAGAAG CTGTCAGCTA 1561 TCTTCCGGGA CTTTCTGAAC CGCTGTCTCG AGATGGATGT GGAGAAGAGA GGTTCAGCTA 1621 AAGAGCTGCT ACAGGTGAGA AAACTGAGGT TTCAAGTGTT TAGTAACTTT TCCATGATAG 1681 CTGCATCAAT TCCTGAAGAT TGCCAAGCCC CTCTCCAGCC TCACTCCACT GATTGCTGCA 1741 GCTAAGGAGG CAACAAAGAA CAATCACTAA AAGGGTGGGC GCGCCGACCC AGCTTTCTTG 1801 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 1861 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 1921 GGAAAGGACG AATAGGCAGG ATTGATTGGA CAGGAACGCG TTAAGTCgac aatcaacctc 1981 tggattacaa aatttgtgaa agatt