Construct: ORF TRCN0000488545

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019785.1_s317c1
DNA Barcode:
CTCATACCTGATAGCGCAGGAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STRADB (55437)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488545
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55437 STRADB STE20 related adaptor beta NM_018571.6 99.8% 99.7% 756C>T;1254_1255insG
2 human 55437 STRADB STE20 related adaptor beta NM_001206864.2 89.5% 88.7% (many diffs)
3 human 55437 STRADB STE20 related adaptor beta XM_005246669.2 68% 66.1% 756C>T;823_824ins206;855_856ins194
4 human 55437 STRADB STE20 related adaptor beta XM_017004438.1 66.8% 66.8% 0_1ins414;342C>T;840_841insG
5 mouse 227154 Stradb STE20-related kinase adapto... NM_172656.5 89.5% 90.6% (many diffs)
6 mouse 227154 Stradb STE20-related kinase adapto... XR_373251.2 40.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1329
ORF length:
1257
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtctctt ttggattgct tctgcacttc aagaacacaa gttgaatcac
121tcagacctga aaaacagtct gaaaccagta tccatcaata cttggttgat gagccaaccc
181tttcctggtc acgtccatcc actagagcca gtgaagtact atgttccacc aacgtttctc
241actatgagct ccaagtagaa ataggaagag gatttgacaa cttgacttct gtccatcttg
301cacggcatac tcccacagga acactggtaa ctataaaaat tacaaatctg gaaaactgca
361atgaagaacg cctgaaagct ttacagaaag ccgtgattct atcccacttt ttccggcatc
421ccaatattac aacttattgg acagttttca ctgttggcag ctggctttgg gttatttctc
481catttatggc ctatggttca gcaagtcaac tcttgaggac ctattttcct gaaggaatga
541gtgaaacttt aataagaaac attctctttg gagccgtgag agggttgaac tatctgcacc
601aaaatggctg tattcacagg agtattaaag ccagccatat cctcatttct ggtgatggcc
661tagtgaccct ctctggcctg tcccatctgc atagtttggt taagcatgga cagaggcata
721gggctgtgta tgatttccca cagttcagca catcagtgca gccgtggctg agtccagaac
781tactgagaca ggatttacat gggtataatg tgaagtcaga tatttatagt gttgggatta
841cagcatgtga attagccagt gggcaggtgc ctttccagga catgcataga actcagatgc
901tgttacagaa actgaaaggt cctccttata gcccattgga tatcagtatt ttccctcaat
961cagaatccag aatgaaaaat tcccagtcag gtgtagactc tgggattGGA GAAAGTGTGC
1021TTGTCTCCAG TGGAACTCAC ACAGTAAATA GTGACCGATT ACACACACCA TCCTCAAAAA
1081CTTTCTCTCC TGCCTTCTTT AGCTTGGTAC AGCTCTGTTT GCAACAAGAT CCTGAGAAAA
1141GGCCATCAGC AAGCAGTTTA TTGTCCCATG TTTTCTTCAA ACAGATGAAA GAAGAAAGCC
1201AGGATTCAAT ACTTTCACTG TTGCCTCCTG CTTATAACAA GCCATCAATA TCATTGCCTC
1261CAGTGTTACC TTGGACTGAG CCAGAATGTG ATTTTCCTGA TGAAAAAGAC TCATACTGGG
1321AATTCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1381TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1441CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCATACCT GATAGCGCAG GAGCCACGCG
1501TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)