Construct: ORF TRCN0000488558
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021495.1_s317c1
- DNA Barcode:
- ATTCACAATAGGCGGCACCCTTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IGFBP2 (3485)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488558
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3485 | IGFBP2 | insulin like growth factor ... | NM_000597.3 | 99% | 99% | 44_45insGCCGCTGCT |
| 2 | human | 3485 | IGFBP2 | insulin like growth factor ... | NM_001313990.2 | 55.1% | 53.9% | (many diffs) |
| 3 | human | 3485 | IGFBP2 | insulin like growth factor ... | NM_001313992.1 | 48.4% | 48.4% | 0_1ins507 |
| 4 | human | 3485 | IGFBP2 | insulin like growth factor ... | NM_001313993.1 | 48.4% | 48.4% | 0_1ins507 |
| 5 | mouse | 16008 | Igfbp2 | insulin-like growth factor ... | NM_008342.3 | 81.4% | 82% | (many diffs) |
| 6 | mouse | 16008 | Igfbp2 | insulin-like growth factor ... | NM_001310659.1 | 42.9% | 44.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1053
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gctgccgaga gtgggctgcc ccgcgctgcc gctgccgccg ccgccgctgc 121 tgccgctgct gccgctgctg ctgctgctac tgggcgcgag tggcggcggc ggcggggcgc 181 gcgcggaggt gctgttccgc tgcccgccct gcacacccga gcgcctggcc gcctgcgggc 241 ccccgccggt tgcgccgccc gccgcggtgg ccgcagtggc cggaggcgcc cgcatgccat 301 gcgcggagct cgtccgggag ccgggctgcg gctgctgctc ggtgtgcgcc cggctggagg 361 gcgaggcgtg cggcgtctac accccgcgct gcggccaggg gctgcgctgc tatccccacc 421 cgggctccga gctgcccctg caggcgctgg tcatgggcga gggcacttgt gagaagcgcc 481 gggacgccga gtatggcgcc agcccggagc aggttgcaga caatggcgat gaccactcag 541 aaggaggcct ggtggagaac cacgtggaca gcaccatgaa catgttgggc gggggaggca 601 gtgctggccg gaagcccctc aagtcgggta tgaaggagct ggccgtgttc cgggagaagg 661 tcactgagca gcaccggcag atgggcaagg gtggcaagca tcaccttggc ctggaggagc 721 ccaagaagct gcgaCCACCC CCTGCCAGGA CTCCCTGCCA ACAGGAACTG GACCAGGTCC 781 TGGAGCGGAT CTCCACCATG CGCCTTCCGG ATGAGCGGGG CCCTCTGGAG CACCTCTACT 841 CCCTGCACAT CCCCAACTGT GACAAGCATG GCCTGTACAA CCTCAAACAG TGCAAGATGT 901 CTCTGAACGG GCAGCGTGGG GAGTGCTGGT GTGTGAACCC CAACACCGGG AAGCTGATCC 961 AGGGAGCCCC CACCATCCGG GGGGACCCCG AGTGTCATCT CTTCTACAAT GAGCAGCAGG 1021 AGGCTCGCGG GGTGCACACC CAGCGGATGC AGTAGTAGGA CCCAGCTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGAATTCAC AATAGGCGGC ACCCTTGTAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt