Construct: ORF TRCN0000488563
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021217.1_s317c1
- DNA Barcode:
- ACTTACCTTGTCAGGAAGGGAAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HOMER2 (9455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488563
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9455 | HOMER2 | homer scaffold protein 2 | NM_004839.4 | 100% | 100% | |
| 2 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_005272449.4 | 99.9% | 99.7% | 4A>G |
| 3 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_005272448.5 | 97.1% | 96.6% | 1_12del;17_34del |
| 4 | human | 9455 | HOMER2 | homer scaffold protein 2 | NM_199330.3 | 96.8% | 96.8% | 388_420del |
| 5 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_006720776.4 | 96.7% | 96.6% | 4A>G;388_420del |
| 6 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_011522233.3 | 94.6% | 94.7% | 1_23delinsA;27_28delAA;412_444del |
| 7 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_006720775.3 | 94.2% | 93.6% | 1_12del;17_34del;418_450del |
| 8 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_011522231.3 | 94.2% | 93.6% | 1_12del;17_34del;418_450del |
| 9 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_011522232.2 | 94.2% | 93.6% | 1_12del;17_34del;418_450del |
| 10 | human | 9455 | HOMER2 | homer scaffold protein 2 | XM_011522234.3 | 90.1% | 90.1% | 0_1ins72;316_348del |
| 11 | mouse | 26557 | Homer2 | homer scaffolding protein 2 | NM_001164086.1 | 89.8% | 94.4% | (many diffs) |
| 12 | mouse | 26557 | Homer2 | homer scaffolding protein 2 | NM_011983.2 | 87% | 91.5% | (many diffs) |
| 13 | mouse | 26557 | Homer2 | homer scaffolding protein 2 | NM_001164087.1 | 70.2% | 74% | (many diffs) |
| 14 | mouse | 26557 | Homer2 | homer scaffolding protein 2 | XM_006540909.1 | 70.2% | 74% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1101
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggagaa cagcccatct tcaccacccg agcgcatgtc ttccagattg 121 accccaacac caagaagaac tggatgcctg cgagcaagca ggcggtcacc gtttcctact 181 tctatgatgt cacaaggaac agctatcgga tcatcagtgt ggacggagcc aaggtgatca 241 taaacagcac aatcacaccg aatatgacct tcaccaaaac gtcacagaag tttgggcagt 301 gggccgacag cagagccaac acagtgtttg gtttggggtt ttcctctgag cagcagctga 361 caaagtttgc agagaaattc caggaggtga aagaagctgc caagatagcc aaagacaaga 421 cgcaggagaa aatcgagacc tcaagtaatc attcccaagc atccagtgtc aacgggacgg 481 acgatgaaaa ggcctctcac gccggtccag ccaacacaca cctgaagtct gagaatgaca 541 agctgaagat tgccttgacg cagagcgcag ccaacgtgaa gaagtgggag atcgagctgc 601 agacccttcg ggagagcaat gcacggctga ccacagcact gcaggagtcg gcagccagtg 661 tggagcagtg gaagaggcag ttctccatct gccgtgatga gaatgaccgg ctccgcaaca 721 agattgatga gctggaagaa CAATGCAGTG AGATCAACAG AGAGAAGGAG AAGAACACGC 781 AGCTGAAGAG GAGGATCGAG GAGCTGGAGG CAGAGCTCCG AGAAAAGGAG ACAGAGCTGA 841 AAGATCTCCG AAAACAAAGT GAAATCATAC CTCAGCTCAT GTCAGAGTGC GAATATGTCT 901 CTGAGAAGCT AGAGGCGGCA GAGAGAGACA ATCAAAACCT GGAAGACAAA GTGCGTTCCT 961 TAAAGACAGA CATTGAGGAG AGCAAATACC GACAGCGCCA CCTGAAGGTG GAGTTGAAGA 1021 GCTTCCTGGA GGTGCTGGAC GGGAAGATTG ACGACCTGCA TGACTTCCGC CGAGGGCTCT 1081 CCAAGCTGGG CACCGATAAC TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CTTACCTTGT 1261 CAGGAAGGGA AGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att