Construct: ORF TRCN0000488580
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020182.1_s317c1
- DNA Barcode:
- TGTTTACCTTTCTAAGTGAGACGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K5 (5607)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488580
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5607 | MAP2K5 | mitogen-activated protein k... | NM_145160.3 | 100% | 100% | |
| 2 | human | 5607 | MAP2K5 | mitogen-activated protein k... | NM_002757.4 | 97.7% | 97.7% | 1042_1043ins30 |
| 3 | human | 5607 | MAP2K5 | mitogen-activated protein k... | NM_001206804.2 | 91.3% | 90.2% | (many diffs) |
| 4 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XM_024449988.1 | 82.8% | 82.8% | 0_1ins231 |
| 5 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XM_011521787.3 | 64.2% | 63.6% | (many diffs) |
| 6 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XM_011521788.3 | 63% | 62.9% | 848_849delGGins497 |
| 7 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XM_017022414.2 | 59.3% | 59.3% | 797_798delCA;801_802ins545 |
| 8 | human | 5607 | MAP2K5 | mitogen-activated protein k... | XR_001751355.2 | 48.6% | 1_651del;1449_1450ins49;1621_1622ins325 | |
| 9 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | NM_011840.2 | 91% | 97.7% | (many diffs) |
| 10 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | XM_006511145.2 | 89% | 95.7% | (many diffs) |
| 11 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | XM_006511146.2 | 88.8% | 95.5% | (many diffs) |
| 12 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | XM_011242740.2 | 85.3% | 91% | (many diffs) |
| 13 | mouse | 23938 | Map2k5 | mitogen-activated protein k... | XM_011242741.2 | 72.7% | 78.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1416
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctgtgg ctagcccttg gcccctttcc tgccatggag aaccaggtgc 121 tggtaattcg catcaagatc ccaaatagtg gcgcggtgga ctggacagtg cactccgggc 181 cgcagttact cttcagggat gtgctggatg tgataggcca ggttctgcct gaagcaacaa 241 ctacagcatt tgaatatgaa gatgaagatg gtgatcgaat tacagtgaga agtgatgagg 301 aaatgaaggc aatgctgtca tattattatt ccacagtaat ggaacagcaa gtaaatggac 361 agttaataga gcctctgcag atatttccaa gagcctgcaa gcctcctggg gaacggaaca 421 tacatggcct gaaggtgaat actcgggccg gaccctctca acacagcagc ccagcagtct 481 cagattcact tccaagcaat agcttaaaga agtcttctgc tgaactgaaa aaaatactag 541 ccaatggcca gatgaatgaa caagacatac gatatcggga cactcttggt catggcaacg 601 gaggcacagt ctacaaagca tatcatgtcc cgagtgggaa aatattagct gtaaaggtca 661 tactactaga tattacactg gaacttcaga agcaaattat gtctgaattg gaaattcttt 721 ataagtgcga ttcatcatat atcattggat tttatggagc attttttgta gaaaacagga 781 tttcaatatg tacagaattc atggatgggg gatctttgga tgtatatagg aaaatgccag 841 aacatgtcct tggaagaatt gcagtagcag ttgttaaagg ccttacttat ttgtggagtt 901 taaagatttt acatagagac gtgaagccct ccaatatgct agtaaacaca agaggacagg 961 ttaagctgtg tgattttgga gttagcactc agctggtgaa ttctatagcc aagacgtatg 1021 ttggaacaaa tgcttatatg gcgcctgaaa ggatttcagg ggagcagtat ggaattcatt 1081 ctgatgtctg gagcttagga atctctttta tggagcttgc tcttgggagg tttccatatc 1141 cTCAGATTCA GAAAAACCAG GGATCTTTAA TGCCTCTCCA GCTTCTGCAG TGCATTGTTG 1201 ATGAGGATTC GCCCGTCCTT CCAGTTGGAG AGTTCTCGGA GCCATTTGTA CATTTCATCA 1261 CTCAGTGTAT GCGAAAACAG CCAAAAGAAA GGCCAGCACC TGAAGAATTG ATGGGCCACC 1321 CGTTCATCGT GCAGTTCAAT GATGGAAATG CCGCCGTGGT GTCCATGTGG GTGTGCCGGG 1381 CGCTGGAGGA GAGGCGGAGC CAGCAGGGGC CCCCGTGAGA CCCAGCTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGATGTTTA CCTTTCTAAG TGAGACGGAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt