Construct: ORF TRCN0000488624
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019474.1_s317c1
- DNA Barcode:
- AAAATCAGCTTATGCATAATTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR2A (3356)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488624
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3356 | HTR2A | 5-hydroxytryptamine recepto... | NM_000621.4 | 99.9% | 99.7% | 1413_1414insG |
| 2 | human | 3356 | HTR2A | 5-hydroxytryptamine recepto... | NM_001165947.2 | 78.5% | 72.5% | (many diffs) |
| 3 | mouse | 15558 | Htr2a | 5-hydroxytryptamine (seroto... | NM_172812.2 | 87.4% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1491
- ORF length:
- 1416
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggat attctttgtg aagaaaatac ttctttgagc tcaactacga 121 actccctaat gcaattaaat gatgacacca ggctctacag taatgacttt aactccggag 181 aagctaacac ttctgatgca tttaactgga cagtcgactc tgaaaatcga accaaccttt 241 cctgtgaagg gtgcctctca ccgtcgtgtc tctccttact tcatctccag gaaaaaaact 301 ggtctgcttt actgacagcc gtagtgatta ttctaactat tgctggaaac atactcgtca 361 tcatggcagt gtccctagag aaaaagctgc agaatgccac caactatttc ctgatgtcac 421 ttgccatagc tgatatgctg ctgggtttcc ttgtcatgcc cgtgtccatg ttaaccatcc 481 tgtatgggta ccggtggcct ctgccgagca agctttgtgc agtctggatt tacctggacg 541 tgctcttctc cacggcctcc atcatgcacc tctgcgccat ctcgctggac cgctacgtcg 601 ccatccagaa tcccatccac cacagccgct tcaactccag aactaaggca tttctgaaaa 661 tcattgctgt ttggaccata tcagtaggta tatccatgcc aataccagtc tttgggctac 721 aggacgattc gaaggtcttt aaggagggga gttgcttact cgccgatgat aactttgtcc 781 tgatcggctc ttttgtgtca tttttcattc ccttaaccat catggtgatc acctactttc 841 taactatcaa gtcactccag aaagaagcta ctttgtgtgt aagtgatctt ggcacacggg 901 ccaaattagc ttctttcagc ttcctccctc agagttcttt gtcttcagaa aagctcttcc 961 agcggtcgat ccatagggag ccagggtcct acacaggcag gaggactatg cagtccatca 1021 gcaatgagca aaaggcatgc aaggtgctgg gcatcgtctt cttcctgttt gtggtgatgt 1081 ggtgcccttt cttcatcaca aacatcatgg ccgtcatctg caaagagtcc tgcaatgagg 1141 atgtcattGG GGCCCTGCTC AATGTGTTTG TTTGGATCGG TTATCTCTCT TCAGCAGTCA 1201 ACCCACTAGT CTACACACTG TTCAACAAGA CCTATAGGTC AGCCTTTTCA CGGTATATTC 1261 AGTGTCAGTA CAAGGAAAAC AAAAAACCAT TGCAGTTAAT TTTAGTGAAC ACAATACCGG 1321 CTTTGGCCTA CAAGTCTAGC CAACTTCAAA TGGGACAAAA AAAGAATTCA AAGCAAGATG 1381 CCAAGACAAC AGATAATGAC TGCTCAATGG TTGCTCTAGG AAAGCAGCAT TCTGAAGAGG 1441 CTTCTAAAGA CAATAGCGAC GGAGTGAATG AAAAGGTGAG CTGTGTGGAC CCAGCTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGAAAAATCA GCTTATGCAT AATTCGTACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt