Construct: ORF TRCN0000488632

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021509.1_s317c1
DNA Barcode:
CAAAGCCTTACGGATTTATGACCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
USP7 (7874)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488632
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7874 USP7 ubiquitin specific peptidase 7 NM_003470.3 100% 100%
2 human 7874 USP7 ubiquitin specific peptidase 7 NM_001286457.2 98.3% 98% (many diffs)
3 human 7874 USP7 ubiquitin specific peptidase 7 XM_017023652.1 97.1% 95.1% (many diffs)
4 human 7874 USP7 ubiquitin specific peptidase 7 XM_017023653.2 95.4% 93.1% (many diffs)
5 human 7874 USP7 ubiquitin specific peptidase 7 NM_001321858.1 94.7% 94.7% 0_1ins174
6 human 7874 USP7 ubiquitin specific peptidase 7 NM_001286458.2 91% 91% 0_1ins297
7 human 7874 USP7 ubiquitin specific peptidase 7 NR_135826.1 61.4% (many diffs)
8 mouse 252870 Usp7 ubiquitin specific peptidase 7 NM_001003918.2 92.5% 99% (many diffs)
9 mouse 252870 Usp7 ubiquitin specific peptidase 7 XM_006522139.3 90.8% 97.1% (many diffs)
10 mouse 252870 Usp7 ubiquitin specific peptidase 7 XM_006522138.3 88.1% 92.1% (many diffs)
11 mouse 252870 Usp7 ubiquitin specific peptidase 7 XM_006522141.3 83.9% 90.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3375
ORF length:
3306
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gaaccaccag cagcagcagc agcagcagaa agcgggcgag cagcagttga
121gcgagcccga ggacatggag atggaagcgg gagatacaga tgacccacca agaattactc
181agaaccctgt gatcaatggg aatgtggccc tgagtgatgg acacaacacc gcggaggagg
241acatggagga tgacaccagt tggcgctccg aggcaacctt tcagttcact gtggagcgct
301tcagcagact gagtgagtcg gtccttagcc ctccgtgttt tgtgcgaaat ctgccatgga
361agattatggt gatgccacgc ttttatccag acagaccaca ccaaaaaagc gtaggattct
421ttctccagtg caatgctgaa tctgattcca cgtcatggtc ttgccatgca caagcagtgc
481tgaagataat aaattacaga gatgatgaaa agtcgttcag tcgtcgtatt agtcatttgt
541tcttccataa agaaaatgat tggggatttt ccaattttat ggcctggagt gaagtgaccg
601atcctgagaa aggatttata gatgatgaca aagttacctt tgaagtcttt gtacaggcgg
661atgctcccca tggagttgcg tgggattcaa agaagcacac aggctacgtc ggcttaaaga
721atcagggagc gacttgttac atgaacagcc tgctacagac gttatttttc acgaatcagc
781tacgaaaggc tgtgtacatg atgccaaccg agggggatga ttcgtctaaa agcgtccctt
841tagcattaca aagagtgttc tatgaattac agcatagtga taaacctgta ggaacaaaaa
901agttaacaaa gtcatttggg tgggaaactt tagatagctt catgcaacat gatgttcagg
961agctttgtcg agtgttgctc gataatgtgg aaaataagat gaaaggcacc tgtgtagagg
1021gcaccatacc caaattattc cgcggcaaaa tggtgtccta tatccagtgt aaagaagtag
1081actatcggtc tgatagaaga gaagattatt atgatatcca gctaagtatc aaaggaaaga
1141aaaatatatt tgaatcattt gtggattatg tggcagtaga acagctcgat ggggacaata
1201aatacgacgc tggggaacat ggcttacagg aagcagagaa aggtgtgaaa ttcctaacat
1261tgccaccagt gttacatcta caactgatga gatttatgta tgaccctcag acggaccaaa
1321atatcaagat caatgatagg tttgaattcc cagagcagtt accacttgat gaatttttgc
1381aaaaaacaga tcctaaggac cctgcaaatt atattcttca tgcagtcctg gttcatagtg
1441gagataatca tggtggacat tatgtggttt atctaaaccc caaaggggat ggcaaatggt
1501gtaaatttga tgacgacgtg gtgtcaaggt gtactaaaga ggaagcaatt gagcacaatt
1561atgggggtca cgatgacgac ctgtctgttc gacactgcac taatgcttac atgttagtct
1621acatcaggga atcaaaactg agtgaagttt tacaggcggt caccgaccat gatattcctc
1681agcagttggt ggagcgatta caagaagaga aaaggatcga ggctcagaag cggaaggagc
1741ggcaggaagc ccatctctat atgcaagtgc agatagtcgc agaggaccag ttttgtggcc
1801accaagggaa tgacatgtac gatgaagaaa aagtgaaata cactgtgttc aaagtattga
1861agaactcctc gcttgctgag tttgttcaga gcctctctca gaccatggga tttccacaag
1921atcaaattcg attgtggccc atgcaagcaa ggagtaatgg aacaaaacga ccagcaatgt
1981tagataatga agccgacggc aataaaacaa tgattgagct cagtgataat gaaaaccctt
2041ggacaatatt cctggaaaca gttgatcccg agctggctgc tagtggagcg accttaccca
2101agtttgataa agatcatgat gtaatgttat ttttgaagat gtatgatccc aaaacgcgga
2161gcttgaatta ctgtgggcat atctacacac caatatcctg taaaatacgt gacttgctcc
2221cagttatgtg tgacagagca ggatttattc aagatactag ccttatcctc tatgaggaag
2281ttaaaccgaa tttaacagag agaattcagg actatgacgt gtctcttgat aaagcccttg
2341atgaactaat ggatggtgac atcatagtat ttcagaagga tgaccctgaa aatgataaca
2401gtgaattacc caccgcaaag gagtatttcc gagatctcta ccaccgcgtt gatgtcattt
2461tctgtgataa aacaatccct aatgatcctg gatttgtggt tacgttatca aatagaatga
2521attattttca ggttgcaaag acagttgcac agaggctcaa cacagatcca atgttgctgc
2581agtttttcaa gtctcaaggt tatagggatg gcccaggtaa tcctcttaga cataattatg
2641aaggtacttt aagagatctt ctacagttct tcaagcctag acaacctaag aaactttact
2701atcagcagct taagatgaaa atcacagact ttgagaacag gcgaagtttt aaatgtatat
2761ggttaaacag ccaatttagg gaagaggaaa taacactata tccagacaag catgggtgtg
2821tccgggacct gttagaagaa tgtaaaaagg ccgtggagct tggggagaaa gcatcaggga
2881aacttaggct gctagaaatt gtaagctaca aaatcattgg tgttcatcaa gaagatgaac
2941tattagaatg tttatctcct gcaacgagcc ggacgtttcg aatagaggaa atccctttgg
3001accaggtgga catagacaaa gagaatgaga tgcttgtcac agtggcgcat ttccacaaag
3061aggTCTTCGG AACGTTCGGA ATCCCGTTTT TGCTGAGGAT ACACCAGGGC GAGCATTTTC
3121GAGAAGTGAT GAAGCGAATC CAGAGCCTGC TGGACATCCA GGAGAAGGAG TTTGAGAAGT
3181TTAAATTTGC AATTGTAATG ATGGGCCGAC ACCAGTACAT AAATGAAGAC GAGTATGAAG
3241TAAATTTGAA AGACTTTGAG CCACAGCCCG GTAATATGTC TCATCCTCGG CCTTGGCTAG
3301GGCTCGACCA CTTCAACAAA GCCCCAAAGA GGAGTCGCTA CACTTACCTT GAAAAGGCCA
3361TTAAAATCCA TAACTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
3421TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
3481GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAAAGCC TTACGGATTT
3541ATGACCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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