Construct: ORF TRCN0000488632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021509.1_s317c1
- DNA Barcode:
- CAAAGCCTTACGGATTTATGACCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- USP7 (7874)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7874 | USP7 | ubiquitin specific peptidase 7 | NM_003470.3 | 100% | 100% | |
| 2 | human | 7874 | USP7 | ubiquitin specific peptidase 7 | NM_001286457.2 | 98.3% | 98% | (many diffs) |
| 3 | human | 7874 | USP7 | ubiquitin specific peptidase 7 | XM_017023652.1 | 97.1% | 95.1% | (many diffs) |
| 4 | human | 7874 | USP7 | ubiquitin specific peptidase 7 | XM_017023653.2 | 95.4% | 93.1% | (many diffs) |
| 5 | human | 7874 | USP7 | ubiquitin specific peptidase 7 | NM_001321858.1 | 94.7% | 94.7% | 0_1ins174 |
| 6 | human | 7874 | USP7 | ubiquitin specific peptidase 7 | NM_001286458.2 | 91% | 91% | 0_1ins297 |
| 7 | human | 7874 | USP7 | ubiquitin specific peptidase 7 | NR_135826.1 | 61.4% | (many diffs) | |
| 8 | mouse | 252870 | Usp7 | ubiquitin specific peptidase 7 | NM_001003918.2 | 92.5% | 99% | (many diffs) |
| 9 | mouse | 252870 | Usp7 | ubiquitin specific peptidase 7 | XM_006522139.3 | 90.8% | 97.1% | (many diffs) |
| 10 | mouse | 252870 | Usp7 | ubiquitin specific peptidase 7 | XM_006522138.3 | 88.1% | 92.1% | (many diffs) |
| 11 | mouse | 252870 | Usp7 | ubiquitin specific peptidase 7 | XM_006522141.3 | 83.9% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3375
- ORF length:
- 3306
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaaccaccag cagcagcagc agcagcagaa agcgggcgag cagcagttga 121 gcgagcccga ggacatggag atggaagcgg gagatacaga tgacccacca agaattactc 181 agaaccctgt gatcaatggg aatgtggccc tgagtgatgg acacaacacc gcggaggagg 241 acatggagga tgacaccagt tggcgctccg aggcaacctt tcagttcact gtggagcgct 301 tcagcagact gagtgagtcg gtccttagcc ctccgtgttt tgtgcgaaat ctgccatgga 361 agattatggt gatgccacgc ttttatccag acagaccaca ccaaaaaagc gtaggattct 421 ttctccagtg caatgctgaa tctgattcca cgtcatggtc ttgccatgca caagcagtgc 481 tgaagataat aaattacaga gatgatgaaa agtcgttcag tcgtcgtatt agtcatttgt 541 tcttccataa agaaaatgat tggggatttt ccaattttat ggcctggagt gaagtgaccg 601 atcctgagaa aggatttata gatgatgaca aagttacctt tgaagtcttt gtacaggcgg 661 atgctcccca tggagttgcg tgggattcaa agaagcacac aggctacgtc ggcttaaaga 721 atcagggagc gacttgttac atgaacagcc tgctacagac gttatttttc acgaatcagc 781 tacgaaaggc tgtgtacatg atgccaaccg agggggatga ttcgtctaaa agcgtccctt 841 tagcattaca aagagtgttc tatgaattac agcatagtga taaacctgta ggaacaaaaa 901 agttaacaaa gtcatttggg tgggaaactt tagatagctt catgcaacat gatgttcagg 961 agctttgtcg agtgttgctc gataatgtgg aaaataagat gaaaggcacc tgtgtagagg 1021 gcaccatacc caaattattc cgcggcaaaa tggtgtccta tatccagtgt aaagaagtag 1081 actatcggtc tgatagaaga gaagattatt atgatatcca gctaagtatc aaaggaaaga 1141 aaaatatatt tgaatcattt gtggattatg tggcagtaga acagctcgat ggggacaata 1201 aatacgacgc tggggaacat ggcttacagg aagcagagaa aggtgtgaaa ttcctaacat 1261 tgccaccagt gttacatcta caactgatga gatttatgta tgaccctcag acggaccaaa 1321 atatcaagat caatgatagg tttgaattcc cagagcagtt accacttgat gaatttttgc 1381 aaaaaacaga tcctaaggac cctgcaaatt atattcttca tgcagtcctg gttcatagtg 1441 gagataatca tggtggacat tatgtggttt atctaaaccc caaaggggat ggcaaatggt 1501 gtaaatttga tgacgacgtg gtgtcaaggt gtactaaaga ggaagcaatt gagcacaatt 1561 atgggggtca cgatgacgac ctgtctgttc gacactgcac taatgcttac atgttagtct 1621 acatcaggga atcaaaactg agtgaagttt tacaggcggt caccgaccat gatattcctc 1681 agcagttggt ggagcgatta caagaagaga aaaggatcga ggctcagaag cggaaggagc 1741 ggcaggaagc ccatctctat atgcaagtgc agatagtcgc agaggaccag ttttgtggcc 1801 accaagggaa tgacatgtac gatgaagaaa aagtgaaata cactgtgttc aaagtattga 1861 agaactcctc gcttgctgag tttgttcaga gcctctctca gaccatggga tttccacaag 1921 atcaaattcg attgtggccc atgcaagcaa ggagtaatgg aacaaaacga ccagcaatgt 1981 tagataatga agccgacggc aataaaacaa tgattgagct cagtgataat gaaaaccctt 2041 ggacaatatt cctggaaaca gttgatcccg agctggctgc tagtggagcg accttaccca 2101 agtttgataa agatcatgat gtaatgttat ttttgaagat gtatgatccc aaaacgcgga 2161 gcttgaatta ctgtgggcat atctacacac caatatcctg taaaatacgt gacttgctcc 2221 cagttatgtg tgacagagca ggatttattc aagatactag ccttatcctc tatgaggaag 2281 ttaaaccgaa tttaacagag agaattcagg actatgacgt gtctcttgat aaagcccttg 2341 atgaactaat ggatggtgac atcatagtat ttcagaagga tgaccctgaa aatgataaca 2401 gtgaattacc caccgcaaag gagtatttcc gagatctcta ccaccgcgtt gatgtcattt 2461 tctgtgataa aacaatccct aatgatcctg gatttgtggt tacgttatca aatagaatga 2521 attattttca ggttgcaaag acagttgcac agaggctcaa cacagatcca atgttgctgc 2581 agtttttcaa gtctcaaggt tatagggatg gcccaggtaa tcctcttaga cataattatg 2641 aaggtacttt aagagatctt ctacagttct tcaagcctag acaacctaag aaactttact 2701 atcagcagct taagatgaaa atcacagact ttgagaacag gcgaagtttt aaatgtatat 2761 ggttaaacag ccaatttagg gaagaggaaa taacactata tccagacaag catgggtgtg 2821 tccgggacct gttagaagaa tgtaaaaagg ccgtggagct tggggagaaa gcatcaggga 2881 aacttaggct gctagaaatt gtaagctaca aaatcattgg tgttcatcaa gaagatgaac 2941 tattagaatg tttatctcct gcaacgagcc ggacgtttcg aatagaggaa atccctttgg 3001 accaggtgga catagacaaa gagaatgaga tgcttgtcac agtggcgcat ttccacaaag 3061 aggTCTTCGG AACGTTCGGA ATCCCGTTTT TGCTGAGGAT ACACCAGGGC GAGCATTTTC 3121 GAGAAGTGAT GAAGCGAATC CAGAGCCTGC TGGACATCCA GGAGAAGGAG TTTGAGAAGT 3181 TTAAATTTGC AATTGTAATG ATGGGCCGAC ACCAGTACAT AAATGAAGAC GAGTATGAAG 3241 TAAATTTGAA AGACTTTGAG CCACAGCCCG GTAATATGTC TCATCCTCGG CCTTGGCTAG 3301 GGCTCGACCA CTTCAACAAA GCCCCAAAGA GGAGTCGCTA CACTTACCTT GAAAAGGCCA 3361 TTAAAATCCA TAACTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3421 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3481 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAAAGCC TTACGGATTT 3541 ATGACCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt