Construct: ORF TRCN0000488639
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020590.1_s317c1
- DNA Barcode:
- GACCAGCTCATGTGGATGCGCCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PNCK (139728)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488639
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_024452339.1 | 28.5% | 25.7% | (many diffs) |
| 2 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366975.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 3 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366976.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 4 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366977.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 5 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366978.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 6 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366979.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 7 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366980.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 8 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_006724809.3 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 9 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531107.2 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 10 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531111.2 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 11 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531112.2 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 12 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_017029279.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 13 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_024452340.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 14 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001135740.1 | 23.6% | 23.6% | 1_51del;119_120ins87;328_1080del |
| 15 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531108.2 | 23.5% | 23.5% | 1_54del;122_123ins87;331_1083del |
| 16 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001039582.3 | 20.2% | 20.2% | 1_249del;317_318ins87;526_1278del |
| 17 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_001199351.1 | 21.5% | 23.9% | (many diffs) |
| 18 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_001199352.1 | 21.5% | 23.9% | (many diffs) |
| 19 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_012040.3 | 21.5% | 23.9% | (many diffs) |
| 20 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_006528344.4 | 21.5% | 23.9% | (many diffs) |
| 21 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_011247692.3 | 21.5% | 23.9% | (many diffs) |
| 22 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_030251530.1 | 21.5% | 23.9% | (many diffs) |
| 23 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_030251531.1 | 21.5% | 23.9% | (many diffs) |
| 24 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953034.1 | 16% | (many diffs) | |
| 25 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_001782761.2 | 15.5% | (many diffs) | |
| 26 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953033.1 | 14% | (many diffs) | |
| 27 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953032.1 | 13.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 435
- ORF length:
- 363
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctgctg ctgaagaaac acacggagga catcagcagc gtctacgaga 121 tccgcgagag gctcggctcg gggccctccc cgctccactc tctctccctc ctgcctcTCC 181 TCTCTTCCCA CTTCCTTCCC ACCTCCCACC GCCCCGTCTG CGGCAGGGGT GCCTTCTCCG 241 AGGTGGTGCT GGCCCAGGAG CGGGGCTCCG CACACCTCGT GGCCCTCAAG TGCATCCCCA 301 AGAAGGCCCT CCGGGGCAAG GAGGCCCTGG TGGAGAACGA GATCGCAGTG CTCCGTAGGA 361 TCAGTCACCC CAACATCGTC GCTCTGGAGG ATGTCCACGA GAGCCCTTCC CACCTCTACC 421 TGGCCATGGA ACTGTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGACCAGC TCATGTGGAT 601 GCGCCAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt