Construct: ORF TRCN0000488675
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021076.1_s317c1
- DNA Barcode:
- CCAATAACTATTTATTTGAATGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FSHR (2492)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488675
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2492 | FSHR | follicle stimulating hormon... | NM_000145.4 | 100% | 100% | |
| 2 | human | 2492 | FSHR | follicle stimulating hormon... | NM_181446.3 | 96.2% | 96.2% | 444_445ins78 |
| 3 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532733.2 | 95.3% | 95.3% | 668_769del |
| 4 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532734.2 | 78.8% | 77.7% | (many diffs) |
| 5 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532735.2 | 62% | 62% | 0_1ins792 |
| 6 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532736.2 | 62% | 62% | 0_1ins792 |
| 7 | human | 2492 | FSHR | follicle stimulating hormon... | XM_011532740.1 | 39% | 39% | 668_769del;957_957delAins1231 |
| 8 | mouse | 14309 | Fshr | follicle stimulating hormon... | NM_013523.3 | 86.2% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2157
- ORF length:
- 2085
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccctg ctcctggtct ctttgctggc attcctgagc ttgggctcag 121 gatgtcatca tcggatctgt cactgctcta acagggtttt tctctgccaa gagagcaagg 181 tgacagagat tccttctgac ctcccgagga atgccattga actgaggttt gtcctcacca 241 agcttcgagt catccaaaaa ggtgcatttt caggatttgg ggacctggag aaaatagaga 301 tctctcagaa tgatgtcttg gaggtgatag aggcagatgt gttctccaac cttcccaaat 361 tacatgaaat tagaattgaa aaggccaaca acctgctcta catcaaccct gaggccttcc 421 agaaccttcc caaccttcaa tatctgttaa tatccaacac aggtattaag caccttccag 481 atgttcacaa gattcattct ctccaaaaag ttttacttga cattcaagat aacataaaca 541 tccacacaat tgaaagaaat tctttcgtgg ggctgagctt tgaaagtgtg attctatggc 601 tgaataagaa tgggattcaa gaaatacaca actgtgcatt caatggaacc caactagatg 661 agctgaatct aagcgataat aataatttag aagaattgcc taatgatgtt ttccacggag 721 cctctggacc agtcattcta gatatttcaa gaacaaggat ccattccctg cctagctatg 781 gcttagaaaa tcttaagaag ctgagggcca ggtcgactta caacttaaaa aagctgccta 841 ctctggaaaa gcttgtcgcc ctcatggaag ccagcctcac ctatcccagc cattgctgtg 901 cctttgcaaa ctggagacgg caaatctctg agcttcatcc aatttgcaac aaatctattt 961 taaggcaaga agttgattat atgactcagg ctaggggtca gagatcctct ctggcagaag 1021 acaatgagtc cagctacagc agaggatttg acatgacgta cactgagttt gactatgact 1081 tatgcaatga agtggttgac gtgacctgct cccctaagcc agatgcattc aacccatgtg 1141 aagatatcat ggggtacaac atcctcagag tcctgatatg gtttatcagc atcctggcca 1201 tcactgggaa catcatagtg ctagtgatcc taactaccag ccaatataaa ctcacagtcc 1261 ccaggttcct tatgtgcaac ctggcctttg ctgatctctg cattggaatc tacctgctgc 1321 tcattgcatc agttgatatc cataccaaga gccaatatca caactatgcc attgactggc 1381 aaactggggc aggctgtgat gctgctggct ttttcactgt ctttgccagt gagctgtcag 1441 tctacactct gacagctatc accttggaaa gatggcatac catcacgcat gccatgcagc 1501 tggactgcaa ggtgcagctc cgccatgctg ccagtgtcat ggtgatgggc tggatttttg 1561 cttttgcagc tgccctcttt cccatctttg gcatcagcag ctacatgaag gtgagcatct 1621 gcctgcccat ggatattgac agccctttgt cacagctgta tgtcatgtcc ctccttgtgc 1681 tcaatgtcct ggcctttgtg gtcatctgtg gctgctatat ccacatctac ctcacagtgc 1741 ggaaccccaa catcgtgtcc tcctctagtg acaccaggat cgccaagcgc atggccatgc 1801 tcatcttcac tgacttcctc tgcatggcac ccatttcttt ctttgccatt TCTGCCTCCC 1861 TCAAGGTGCC CCTCATCACT GTGTCCAAAG CAAAGATTCT GCTGGTTCTG TTTCACCCCA 1921 TCAACTCCTG TGCCAACCCC TTCCTCTATG CCATCTTTAC CAAAAACTTT CGCAGAGATT 1981 TCTTCATTCT GCTGAGCAAG TGTGGCTGCT ATGAAATGCA AGCCCAAATT TATAGGACAG 2041 AAACTTCATC CACTGTCCAC AACACCCATC CAAGGAATGG CCACTGCTCT TCAGCTCCCA 2101 GAGTCACCAG TGGTTCCACT TACATACTTG TCCCTCTAAG TCATTTAGCC CAAAACTAAG 2161 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2221 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2281 TTTATATATC TTGTGGAAAG GACGACCAAT AACTATTTAT TTGAATGATA CGCGTTAAGT 2341 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt