Construct: ORF TRCN0000488675

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021076.1_s317c1
DNA Barcode:
CCAATAACTATTTATTTGAATGAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
FSHR (2492)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488675
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2492 FSHR follicle stimulating hormon... NM_000145.4 100% 100%
2 human 2492 FSHR follicle stimulating hormon... NM_181446.3 96.2% 96.2% 444_445ins78
3 human 2492 FSHR follicle stimulating hormon... XM_011532733.2 95.3% 95.3% 668_769del
4 human 2492 FSHR follicle stimulating hormon... XM_011532734.2 78.8% 77.7% (many diffs)
5 human 2492 FSHR follicle stimulating hormon... XM_011532735.2 62% 62% 0_1ins792
6 human 2492 FSHR follicle stimulating hormon... XM_011532736.2 62% 62% 0_1ins792
7 human 2492 FSHR follicle stimulating hormon... XM_011532740.1 39% 39% 668_769del;957_957delAins1231
8 mouse 14309 Fshr follicle stimulating hormon... NM_013523.3 86.2% 87.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2157
ORF length:
2085
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggccctg ctcctggtct ctttgctggc attcctgagc ttgggctcag
121gatgtcatca tcggatctgt cactgctcta acagggtttt tctctgccaa gagagcaagg
181tgacagagat tccttctgac ctcccgagga atgccattga actgaggttt gtcctcacca
241agcttcgagt catccaaaaa ggtgcatttt caggatttgg ggacctggag aaaatagaga
301tctctcagaa tgatgtcttg gaggtgatag aggcagatgt gttctccaac cttcccaaat
361tacatgaaat tagaattgaa aaggccaaca acctgctcta catcaaccct gaggccttcc
421agaaccttcc caaccttcaa tatctgttaa tatccaacac aggtattaag caccttccag
481atgttcacaa gattcattct ctccaaaaag ttttacttga cattcaagat aacataaaca
541tccacacaat tgaaagaaat tctttcgtgg ggctgagctt tgaaagtgtg attctatggc
601tgaataagaa tgggattcaa gaaatacaca actgtgcatt caatggaacc caactagatg
661agctgaatct aagcgataat aataatttag aagaattgcc taatgatgtt ttccacggag
721cctctggacc agtcattcta gatatttcaa gaacaaggat ccattccctg cctagctatg
781gcttagaaaa tcttaagaag ctgagggcca ggtcgactta caacttaaaa aagctgccta
841ctctggaaaa gcttgtcgcc ctcatggaag ccagcctcac ctatcccagc cattgctgtg
901cctttgcaaa ctggagacgg caaatctctg agcttcatcc aatttgcaac aaatctattt
961taaggcaaga agttgattat atgactcagg ctaggggtca gagatcctct ctggcagaag
1021acaatgagtc cagctacagc agaggatttg acatgacgta cactgagttt gactatgact
1081tatgcaatga agtggttgac gtgacctgct cccctaagcc agatgcattc aacccatgtg
1141aagatatcat ggggtacaac atcctcagag tcctgatatg gtttatcagc atcctggcca
1201tcactgggaa catcatagtg ctagtgatcc taactaccag ccaatataaa ctcacagtcc
1261ccaggttcct tatgtgcaac ctggcctttg ctgatctctg cattggaatc tacctgctgc
1321tcattgcatc agttgatatc cataccaaga gccaatatca caactatgcc attgactggc
1381aaactggggc aggctgtgat gctgctggct ttttcactgt ctttgccagt gagctgtcag
1441tctacactct gacagctatc accttggaaa gatggcatac catcacgcat gccatgcagc
1501tggactgcaa ggtgcagctc cgccatgctg ccagtgtcat ggtgatgggc tggatttttg
1561cttttgcagc tgccctcttt cccatctttg gcatcagcag ctacatgaag gtgagcatct
1621gcctgcccat ggatattgac agccctttgt cacagctgta tgtcatgtcc ctccttgtgc
1681tcaatgtcct ggcctttgtg gtcatctgtg gctgctatat ccacatctac ctcacagtgc
1741ggaaccccaa catcgtgtcc tcctctagtg acaccaggat cgccaagcgc atggccatgc
1801tcatcttcac tgacttcctc tgcatggcac ccatttcttt ctttgccatt TCTGCCTCCC
1861TCAAGGTGCC CCTCATCACT GTGTCCAAAG CAAAGATTCT GCTGGTTCTG TTTCACCCCA
1921TCAACTCCTG TGCCAACCCC TTCCTCTATG CCATCTTTAC CAAAAACTTT CGCAGAGATT
1981TCTTCATTCT GCTGAGCAAG TGTGGCTGCT ATGAAATGCA AGCCCAAATT TATAGGACAG
2041AAACTTCATC CACTGTCCAC AACACCCATC CAAGGAATGG CCACTGCTCT TCAGCTCCCA
2101GAGTCACCAG TGGTTCCACT TACATACTTG TCCCTCTAAG TCATTTAGCC CAAAACTAAG
2161ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2221GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2281TTTATATATC TTGTGGAAAG GACGACCAAT AACTATTTAT TTGAATGATA CGCGTTAAGT
2341Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)

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