Construct: ORF TRCN0000488677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020716.1_s317c1
- DNA Barcode:
- CTAGCTAACCTGACGCCCCCGGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK31 (56164)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56164 | STK31 | serine/threonine kinase 31 | NM_031414.5 | 99.9% | 99.9% | 213G>C |
| 2 | human | 56164 | STK31 | serine/threonine kinase 31 | XM_011515449.3 | 97.8% | 98.2% | (many diffs) |
| 3 | human | 56164 | STK31 | serine/threonine kinase 31 | NM_001260504.2 | 97.7% | 97.6% | 0_1ins69;144G>C |
| 4 | human | 56164 | STK31 | serine/threonine kinase 31 | NM_001260505.2 | 97.7% | 97.6% | 213G>C;2759_2760ins69 |
| 5 | human | 56164 | STK31 | serine/threonine kinase 31 | NM_032944.4 | 97.7% | 97.6% | 0_1ins69;144G>C |
| 6 | human | 56164 | STK31 | serine/threonine kinase 31 | XM_011515450.1 | 97.7% | 97.6% | 0_1ins69;144G>C |
| 7 | human | 56164 | STK31 | serine/threonine kinase 31 | NR_048542.2 | 93% | (many diffs) | |
| 8 | human | 56164 | STK31 | serine/threonine kinase 31 | XM_006715758.4 | 82.4% | 82.4% | 213G>C;480_481ins534 |
| 9 | human | 56164 | STK31 | serine/threonine kinase 31 | XM_011515452.3 | 80.6% | 80.8% | (many diffs) |
| 10 | mouse | 77485 | Stk31 | serine threonine kinase 31 | XM_006506746.2 | 82.5% | 77.1% | (many diffs) |
| 11 | mouse | 77485 | Stk31 | serine threonine kinase 31 | XM_006506748.3 | 82.5% | 77.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3129
- ORF length:
- 3057
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtgggtc cagggtcact cttctagagc ttccgcaacg gaaagtgtga 121 gtttttcagg aattgttcaa atggatgaag atacacatta cgataaagtg gaagatgtgg 181 ttggaagtca catagaagat gcagtaacat tttgggccca gagtatcaat agaaataagg 241 atatcatgaa gattggttgc tcactgtctg aagtttgccc ccacgccagt tcagttttgg 301 ggaatcttga cccaaacaag atttatggtg gattattttc tgaagatcag tgttggtaca 361 gatgcaaagt actgaaaatc atcagcgttg aaaagtgtct ggtgaggtac attgactatg 421 gaaatactga aattctaaat cgatctgata tagttgaaat tcctttggag ctgcagtttt 481 ctagtgttgc caaaaagtat aaactttggg gactacacat tccttctgat caagaagtta 541 cccagtttga tcagggcaca acctttttgg ggagcttgat ttttgaaaag gaaataaaaa 601 tgagaattaa agcaacctct gaagatggaa cagttattgc tcaggctgag tatggcagtg 661 tggatatagg ggaagaggtg cttaagaaag gatttgcaga gaaatgcaga cttgcttcca 721 gaactgacat ctgtgaggaa aaaaaattgg atcctggtca acttgttctc aggaacctca 781 aaagccccat tcctttgtgg gggcatagat caaaccagtc aaccttcagc aggcccaagg 841 ggcacttaag tgagaaaatg actcttgact tgaaggatga aaatgatgca ggcaatctta 901 taacatttcc aaaggaaagt ttggctgttg gtgactttaa tttagggtct aacgtcagcc 961 tggaaaaaat taagcaggac cagaaactga ttgaagaaaa tgaaaaactt aaaacagaga 1021 aggacgctct tcttgaaagt tataaggcgt tagaattgaa agtagagcag attgcccagg 1081 agctgcagca agagaaggca gctgctgtgg atttgactaa ccacttagaa tacactctga 1141 agacctatat agataccaga atgaaaaatc tggcagctaa gatggaaata ctgaaagaaa 1201 tgaggcatgt cgacatcagt gtccgtttcg gaaaagacct ttcagatgct atacaagtgt 1261 tggatgaagg gtgctttact actccagctt ctttgaatgg attagagata atatgggcag 1321 aatacagtct ggctcaggag aatattaaaa cttgtgaata tgtgagtgaa gggaatattt 1381 tgattgccca aagaaatgaa atgcagcaga agctgtacat gtcagtagaa gattttattc 1441 tggaagttga tgagtcatct cttaataaac gcttaaaaac attgcaggat ttgtcagtct 1501 ctttagaagc agtgtatgga caagccaaag aaggagcaaa ttctgatgaa atacttaaaa 1561 aattttatga ctggaagtgt gataaaagag aggagttcac cagtgttaga agtgaaacag 1621 acgcttctct gcaccgtctt gtagcatggt tccaaagaac cttaaaggtt tttgacctat 1681 ctgtggaagg atcactgatt tcagaagacg caatggataa tattgatgaa atcctagaga 1741 agactgagtc aagtgtctgc aaagagctgg agatagctct ggttgatcaa ggtgatgcag 1801 acaaggagat aatttcaaat acatatagtc aagtactgca aaagattcat tcagaggaaa 1861 ggctcattgc cacagtacaa gctaagtaca aggacagtat tgagtttaaa aagcagctta 1921 ttgaatattt aaataagagt cccagtgtgg atcacttgct atccattaag aagacattga 1981 aaagcttaaa agctctactc agatggaaat tggttgaaaa gagtaatttg gaagagtcag 2041 atgatcctga tggctctcaa attgagaaaa taaaagaaga aataactcag ctgcgcaata 2101 atgtctttca ggaaatttat catgagagag aggaatatga gatgctaact agtttggcac 2161 agaaatggtt ccctgagctg cctctgcttc atcctgaaat aggattactc aaatacatga 2221 actctggtgg tctccttaca atgagcttgg aacgagatct tcttgatgct gagcccatga 2281 aggaacttag cagcaagcgt cctttggtac gttctgaggt taatgggcag ataattctgt 2341 taaagggcta ttctgtggat gttgacacag aagccaaggt gattgagaga gcagccacct 2401 accatagagc ttggagagaa gctgaaggag actcagggtt actgccattg atattcctgt 2461 ttttatgtaa gtctgatcct atggcttatc tgatggtccc atactaccct agggcaaacc 2521 tgaatgctgt tcaagccaac atgcctttaa attcagaaga aactttaaag gtcatgaaag 2581 gtgttgccca gggtctgcat acattgcata aggctgacat aattcatgga tcacttcatc 2641 agaacaatgt atttgcttta aaccgtgaac aaggaattgt tggagatttt gacttcacca 2701 aatctgtgag tcagcgagcc tcggtgaaca tgatggttgg tgacttgagt ttgatgtcac 2761 ctgagttgaa aatgggaaaa cctgcTTCTC CAGGTTCAGA CTTATATGCT TATGGCTGCC 2821 TCTTATTATG GCTTTCTGTT CAAAATCAGG AGTTTGAGAT AAATAAAGAT GGAATCCCCA 2881 AAGTGGATCA GTTTCATCTG GATGATAAAG TCAAATCCCT CCTCTGTAGC TTGATATGTT 2941 ATAGAAGTTC AATGACTGCT GAACAAGTTT TAAATGCTGA ATGTTTCTTG ATGCCAAAGG 3001 AGCAATCAGT TCCAAACCCA GAAAAAGATA CTGAATACAC CCTATATAAA AAGGAAGAAG 3061 AAATAAAGAC GGAGAACTTG GATAAATGTA TGGAGAAGAC AAGAAATGGT GAAGCCAACT 3121 TTGATTGTTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3181 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3241 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTA GCTAACCTGA CGCCCCCGGG 3301 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t