Construct: ORF TRCN0000488699
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021690.1_s317c1
- DNA Barcode:
- TCGCATACTTCTTACGAACCCCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NTRK1 (4914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488699
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_001007792.1 | 44.2% | .3% | 1_1270del;1566G>A |
| 2 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_001012331.1 | 42.5% | .6% | 1_1360del;1656G>A |
| 3 | human | 4914 | NTRK1 | neurotrophic receptor tyros... | NM_002529.3 | 42.2% | .6% | 1_1378del;1674G>A |
| 4 | mouse | 18211 | Ntrk1 | neurotrophic tyrosine kinas... | XM_006501124.2 | 37.4% | .3% | (many diffs) |
| 5 | mouse | 18211 | Ntrk1 | neurotrophic tyrosine kinas... | NM_001033124.1 | 37.1% | .3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 88
- ORF end:
- 115
- ORF length:
- 27
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctcggc tgtgctggct ccagaggatg ggctggccat gtccctgcat ttcatgacat 121 tgggtggcag ctccctgtcc cccaccgagg gcaaaggctc tgggctccaa ggccacatca 181 tcgagaaccc acaatacttc agtgatgcct gtgttcacca catcaagcgc cgggacatcg 241 tgctcaagtg ggagctgggg gagggcgcct ttgggaaggt cttccttgct gagtgccaca 301 acctcctgcc tgagcaggac aagatgctgg tggctgtcaa ggcactgaag gaggcgtccg 361 agagtgctcg gcaggacttc caacgtgagg ctgagctgct caccatgctg cagcaccagc 421 acatcgtgcg cttcttcggc gtctgcaccg agggccgccc cctgctcatg gtctttgagt 481 atatgcggca cggggacctc aaccgcttcc tccgatccca tggacctgat gccaagctgc 541 tggctggtgg ggaggatgtg gctccaggcc ccctgggtct ggggcagctg ctggccgtgg 601 ctagccaggt cgctgcgggg atggtgtacc tggcgggtct gcattttgtg caccgggacc 661 tggccacacg caactgtcta gtgggccagg gactggtggt caagattggt gattttggca 721 tgagcaggga tatctacagc accgactatt accgtgtgGG AGGCCGCACC ATGCTGCCCA 781 TTCGCTGGAT GCCGCCCGAG AGCATCCTGT ACCGTAAGTT CACCACCGAG AGCGACGTGT 841 GGAGCTTCGG CGTGGTGCTC TGGGAGATCT TCACCTACGG CAAGCAGCCC TGGTACCAGC 901 TCTCCAACAC GGAGGCAATC GACTGCATCA CGCAGGGACG TGAGTTGGAG CGGCCACGTG 961 CCTGCCCACC AGAGGTCTAC GCCATCATGC GGGGCTGCTG GCAGCGGGAG CCCCAGCAAC 1021 GCCACAGCAT CAAGGATGTG CACGCCCGGC TGCAAGCCCT GGCCCAGGCA CCTCCTGTCT 1081 ACCTGGATGT CCTGGGCTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCGC ATACTTCTTA 1261 CGAACCCCTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt