Construct: ORF TRCN0000488716

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020468.1_s317c1
DNA Barcode:
GGTCGCGAGCCGTTCCGTTTAGGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
STRADA (92335)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488716
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92335 STRADA STE20 related adaptor alpha NM_001003787.4 70.3% 82.9% 1096_1097ins293;1140_1141ins253
2 human 92335 STRADA STE20 related adaptor alpha NM_001363786.1 69% 81.1% 12_13ins24;1072_1073ins293;1116_1117ins253
3 human 92335 STRADA STE20 related adaptor alpha XM_011525466.3 65.7% 92.1% 1096_1097ins589;1209_1210ins41
4 human 92335 STRADA STE20 related adaptor alpha NM_001363787.1 65.5% 76.4% 36_37ins87;1009_1010ins293;1053_1054ins253
5 human 92335 STRADA STE20 related adaptor alpha XM_011525467.3 64.3% 73.7% (many diffs)
6 human 92335 STRADA STE20 related adaptor alpha NM_001003786.3 64.2% 73.7% 12_13ins111;985_986ins293;1029_1030ins253
7 human 92335 STRADA STE20 related adaptor alpha NM_001003788.3 60.8% 69.8% 0_1ins174;922_923ins293;966_967ins253
8 human 92335 STRADA STE20 related adaptor alpha XM_005257799.3 60.8% 69.8% 0_1ins174;922_923ins293;966_967ins253
9 human 92335 STRADA STE20 related adaptor alpha NR_156741.2 59% (many diffs)
10 human 92335 STRADA STE20 related adaptor alpha XR_243688.3 57.7% (many diffs)
11 human 92335 STRADA STE20 related adaptor alpha NM_153335.6 56.7% 89.3% 12_13ins111;1044_1045ins684
12 human 92335 STRADA STE20 related adaptor alpha NM_001363788.1 55.6% 87.7% (many diffs)
13 human 92335 STRADA STE20 related adaptor alpha NM_001165969.2 50.9% 80.2% (many diffs)
14 human 92335 STRADA STE20 related adaptor alpha NM_001363789.1 49.6% 77.1% (many diffs)
15 human 92335 STRADA STE20 related adaptor alpha NM_001165970.2 48.5% 76.3% (many diffs)
16 human 92335 STRADA STE20 related adaptor alpha XM_005257801.5 47.4% 74.5% (many diffs)
17 human 92335 STRADA STE20 related adaptor alpha NM_001363790.1 47% 71.2% (many diffs)
18 human 92335 STRADA STE20 related adaptor alpha NM_001363791.1 46.2% 72.7% (many diffs)
19 human 92335 STRADA STE20 related adaptor alpha XM_017025314.2 42.7% 67% (many diffs)
20 human 92335 STRADA STE20 related adaptor alpha XM_005257803.5 37.8% 51% (many diffs)
21 mouse 72149 Strada STE20-related kinase adapto... NM_001252448.1 62.4% 81.1% (many diffs)
22 mouse 72149 Strada STE20-related kinase adapto... XM_011249270.1 62.4% 81.1% (many diffs)
23 mouse 72149 Strada STE20-related kinase adapto... XM_006534293.3 61.2% 79.3% (many diffs)
24 mouse 72149 Strada STE20-related kinase adapto... XM_006534294.3 57.9% 74.4% (many diffs)
25 mouse 72149 Strada STE20-related kinase adapto... NM_028126.3 56.5% 71.7% (many diffs)
26 mouse 72149 Strada STE20-related kinase adapto... XM_006534295.1 56.5% 71.7% (many diffs)
27 mouse 72149 Strada STE20-related kinase adapto... NM_001252449.1 54.8% 72.5% (many diffs)
28 mouse 72149 Strada STE20-related kinase adapto... XM_006534296.3 53.7% 68.7% (many diffs)
29 mouse 72149 Strada STE20-related kinase adapto... XM_006534297.3 53.7% 68.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1227
ORF length:
1155
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtcattt cttgtaagta aaccagagcg aatcaggcgg tgggtctcgg
121aaaagttcat tgttgagggc ttaagagatt tggaactatt tggagagcag cctccgggtg
181acactcggag aaaaaccaat gatgcgagct cagagtcaat agcatccttc tctaaacagg
241aggtcatgag tagctttctg ccagagggag ggtgttacga gctgctcact gtgataggca
301aaggatttga ggacctgatg actgtgaatc tagcaaggta caaaccaaca ggagagtacg
361tgactgtacg gaggattaac ctagaagctt gttccaatga gatggtaaca ttcttgcagg
421gcgagctgca tgtctccaaa ctcttcaacc atcccaatat cgtgccatat cgagccactt
481ttattgcaga caatgagctg tgggttgtca catcattcat ggcatacggt tctgcaaaag
541atctcatctg tacacacttc atggatggca tgaatgagct ggcgattgct tacatcctgc
601agggggtgct gaaggccctc gactacatcc accacatggg atatgtacac aggagtgtca
661aagccagcca catcctgatc tctgtggatg ggaaggtcta cctgtctggt ttgcgcagca
721acctcagcat gataagccat gggcagcggc agcgagtggt ccacgatttt cccaagtaca
781gtgtcaaggt tctgccgtgg ctcagccccg aggtcctcca gcagaatctc cagggttatg
841atgccaagtc tgacatctac agtgtgggaa tcacagcctg tgaactggcc aacggccatg
901tcccctttaa ggatatgcct gccacccaga tgctgctaga gaaactgaac ggcacagtgc
961cctgcctgtt ggataccagc accatccccg ctgaggagct gaccatgagc ccttcgcgct
1021cagtggccaa ctctggcctg agtgacagcc tgaccaccag caccccccgg ccctccaacg
1081gtgactcgcc ctcccacccc taccaccgaa ccttctcccc ccacttccac cactttgtgg
1141agcagtgcct tcagcgcaac ccggatgcca ggtatccctg ctggcctggg cctgggcttc
1201gggagagcag agggtgctca ggagggtaag gccagggtgt gaagggactt acctcccaaa
1261ggttctgcag gggaatctgg agctacacac aggagggatc agctcctggg tgtgtcagag
1321gccagcctgg ggagctctgg ccactgcttc ccatgagctg agggagaggg agaggggacc
1381cgaggctgag gcataagtgg caggatttcg ggaagctggg gacacggcag tgatgctgcg
1441gtctctcctc ccctttccct ccaggcccag tgccagcacc ctcctgaacc actctttctt
1501caagcaggta tcgtagcccc ttcgttctgg ttctggttct agttctggtt ctaacaactc
1561acaatccctt tagctttctc tcccctccct tTGAATGAGA GAAACTACCC CGCTTCCGAA
1621GCCCCTGAAA GACACTGCTC CTTCCTCTCA TGGAGTTGGC TCCGACAGCC CGTCTGCCAC
1681CAGGCCATGG TTCCTTGCCC CATGGTGTCC TGGGACCCAG AGCAACAGGA TCTGTCACCC
1741ACCTCTCTCT TCTCCCCCAG ATCAAGCGAC GTGCCTCAGA GGCTTTGCCC GAATTGCTTC
1801GTCCTGTCAC CCCCATCACC AATTTTGAGG GCAGCCAGTC TCAGGACCAC AGTGGAATCT
1861TTGGCCTGGT AACAAACCTG GAAGAGCTGG AGGTGGACGA TTGGGAGTTC TAGGACCCAG
1921CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1981ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
2041TATCTTGTGG AAAGGACGAG GTCGCGAGCC GTTCCGTTTA GGTACGCGTT AAGTCgacaa
2101tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)