Construct: ORF TRCN0000488719
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020300.1_s317c1
- DNA Barcode:
- GAACGTGCGGTGTATCGCACCCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CAMK4 (814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488719
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001323374.2 | 99.6% | 99.5% | 1011G>C;1289T>A;1419_1420insTTG |
| 2 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001323375.2 | 99.6% | 99.5% | 1011G>C;1289T>A;1419_1420insTTG |
| 3 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001744.6 | 99.6% | 99.5% | 1011G>C;1289T>A;1419_1420insTTG |
| 4 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001323376.2 | 58% | 58% | (many diffs) |
| 5 | human | 814 | CAMK4 | calcium/calmodulin dependen... | NM_001323377.2 | 58% | 58% | (many diffs) |
| 6 | human | 814 | CAMK4 | calcium/calmodulin dependen... | XR_001742282.2 | 25.4% | (many diffs) | |
| 7 | human | 814 | CAMK4 | calcium/calmodulin dependen... | XR_001742281.2 | 24.9% | (many diffs) | |
| 8 | human | 814 | CAMK4 | calcium/calmodulin dependen... | XR_948303.2 | 13.5% | (many diffs) | |
| 9 | mouse | 12326 | Camk4 | calcium/calmodulin-dependen... | NM_009793.3 | 82.8% | 80.5% | (many diffs) |
| 10 | mouse | 12326 | Camk4 | calcium/calmodulin-dependen... | XM_006525544.3 | 82.8% | 80.5% | (many diffs) |
| 11 | mouse | 12326 | Camk4 | calcium/calmodulin-dependen... | XM_006525546.3 | 82.8% | 80.5% | (many diffs) |
| 12 | mouse | 12326 | Camk4 | calcium/calmodulin-dependen... | XM_006525543.2 | 78.2% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1494
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctcaaa gtcacggtgc cctcctgctc cgcctcgtcc tgctcttcgg 121 tcaccgccag tgcggccccg gggaccgcga gcctcgtccc ggattactgg atcgacggct 181 ccaacaggga tgcgctgagc gatttcttcg aggtggagtc ggagctggga cggggtgcta 241 catccattgt gtacagatgc aaacagaagg ggacccagaa gccttatgct ctcaaagtgt 301 taaagaaaac agtggacaaa aaaatcgtaa gaactgagat aggagttctt cttcgcctct 361 cacatccaaa cattataaaa cttaaagaga tatttgaaac ccctacagaa atcagtctgg 421 tcctagaact cgtcacagga ggagaactgt ttgataggat tgtggaaaag ggatattaca 481 gtgagcgaga tgctgcagat gccgttaaac aaatcctgga ggcagttgct tatctacatg 541 aaaatgggat tgtccatcgt gatctcaaac cagagaatct tctttatgca actccagccc 601 cagatgcacc actcaaaatc gctgattttg gactctctaa aattgtggaa catcaagtgc 661 tcatgaagac agtatgtgga accccagggt actgcgcacc tgaaattctt agaggttgtg 721 cctatggacc tgaggtggac atgtggtctg taggaataat cacctacatc ttactttgtg 781 gatttgaacc attctatgat gaaagaggcg atcagttcat gttcaggaga attctgaatt 841 gtgaatatta ctttatctcc ccctggtggg atgaagtatc tctaaatgcc aaggacttgg 901 tcagaaaatt aattgttttg gatccaaaga aacggctgac tacatttcaa gctctccagc 961 atccgtgggt cacaggtaaa gcagccaatt ttgtacacat ggataccgct caaaagaagc 1021 tccaagaatt caatgcccgg cgtaagctta aggcagcggt gaaggctgtg gtggcctctt 1081 cccgcctggg aagtgccagc agcagccatg gcagcatcca ggagagccac aaggctagcc 1141 gagacccttc tccaatccaa gatggcaacg aggacatgaa agctattcca gaaggagaga 1201 aaattcaagg cgatgggGCC CAAGCCGCAG TTAAGGGGGC ACAGGCTGAG CTGATGAAGG 1261 TGCAAGCCTT AGAGAAAGTT AAAGGTGCAG ATATAAATGC TGAAGAGGCC CCCAAAATGG 1321 TGCCCAAGGC AGTGGAGGAT GGGATAAAGG TGGCTGACCA GGAACTAGAG GAGGGCCTAG 1381 CAGAGGAGAA GCTGAAGACT GTGGAGGAGG CAGCAGCTCC CAGAGAAGGG CAAGGAAGCT 1441 CTGCTGTGGG TTTTGAAGTT CCACAGCAAG ATGTGATCCT GCCAGAGTAC TTGTGAGACC 1501 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1561 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1621 ATATATCTTG TGGAAAGGAC GAGAACGTGC GGTGTATCGC ACCCCGACGC GTTAAGTCga 1681 caatcaacct ctggattaca aaatttgtga aagatt