Construct: ORF TRCN0000488723
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020152.1_s317c1
- DNA Barcode:
- TGCACACGGGTTATTCTTGGCGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TRIB2 (28951)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488723
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28951 | TRIB2 | tribbles pseudokinase 2 | NM_021643.3 | 100% | 100% | |
| 2 | human | 28951 | TRIB2 | tribbles pseudokinase 2 | NR_027303.2 | 27.5% | (many diffs) | |
| 3 | mouse | 217410 | Trib2 | tribbles pseudokinase 2 | NM_144551.5 | 91.6% | 98.2% | (many diffs) |
| 4 | mouse | 217410 | Trib2 | tribbles pseudokinase 2 | XM_006515054.1 | 91.6% | 98.2% | (many diffs) |
| 5 | mouse | 217410 | Trib2 | tribbles pseudokinase 2 | XM_006515055.2 | 74.3% | 70.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1101
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaacata cacaggtcta cccccatcac aatagcgaga tatgggagat 121 cgcggaacaa aacccaggat ttcgaagagt tgtcgtctat aaggtccgcg gagcccagcc 181 agagtttcag cccgaacctc ggctccccga gcccgcccga gactccgaac ttgtcgcatt 241 gcgtttcttg tatcgggaaa tacttattgt tggaacctct ggagggagac cacgtttttc 301 gtgccgtgca tctgcacagc ggagaggagc tggtgtgcaa ggtgtttgat atcagctgct 361 accaggaatc cctggcaccg tgcttttgcc tgtctgctca tagtaacatc aaccaaatca 421 ctgaaattat cctgggtgag accaaagcct atgtgttctt tgagcgaagc tatggggaca 481 tgcattcctt cgtccgcacc tgcaagaagc tgagagagga ggaggcagcc agactgttct 541 accagattgc ctcggcagtg gcccactgcc atgacggggg gctggtgctg cgggacctca 601 agctgcggaa attcatcttt aaggacgaag agaggactcg ggtcaagctg gaaagcctgg 661 aagacgccta cattctgcgg ggagatgatg attccctctc cgacaagcat ggctgcccgg 721 cttacgtaag cccagagatc ttgaaCACCA GTGGCAGCTA CTCGGGCAAA GCAGCCGACG 781 TGTGGAGCCT GGGGGTGATG CTGTACACCA TGTTGGTGGG GCGGTACCCT TTCCATGACA 841 TTGAACCCAG CTCCCTCTTC AGCAAGATCC GGCGTGGCCA GTTCAACATT CCAGAGACTC 901 TGTCGCCCAA GGCCAAGTGC CTCATCCGAA GCATTCTGCG TCGGGAGCCC TCAGAGCGGC 961 TGACCTCGCA GGAAATTCTG GACCATCCTT GGTTTTCTAC AGATTTTAGC GTCTCGAATT 1021 CAGCATATGG TGCTAAGGAA GTGTCTGACC AGCTGGTGCC GGACGTCAAC ATGGAAGAGA 1081 ACTTGGACCC TTTCTTTAAC TGAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GCACACGGGT 1261 TATTCTTGGC GTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att