Transcript: Human NR_027303.2

Homo sapiens tribbles pseudokinase 2 (TRIB2), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2019-06-11
Taxon:
Homo sapiens (human)
Gene:
TRIB2 (28951)
Length:
2777
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_027303.2
NBCI Gene record:
TRIB2 (28951)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_027303.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000007142 CGTGGACTCTAGTATGTAAAT pLKO.1 2353 3UTR 100% 13.200 18.480 N TRIB2 n/a
2 TRCN0000356139 GCATCGCACTGTTAGCATTTA pLKO_005 1199 3UTR 100% 13.200 18.480 N TRIB2 n/a
3 TRCN0000356137 CAACCACCTAGCTGGTAATTA pLKO_005 1282 3UTR 100% 15.000 12.000 N TRIB2 n/a
4 TRCN0000199912 GACCTCAAGCTGCGGAAATTC pLKO.1 328 3UTR 100% 13.200 9.240 N TRIB2 n/a
5 TRCN0000007144 GCTGCGGAAATTCATCTTTAA pLKO.1 336 3UTR 100% 13.200 9.240 N TRIB2 n/a
6 TRCN0000196978 GAAGAGAACTTGGACCCTTTC pLKO.1 808 3UTR 100% 6.000 4.200 N TRIB2 n/a
7 TRCN0000007146 CGTCAACATGGAAGAGAACTT pLKO.1 798 3UTR 100% 4.950 3.465 N TRIB2 n/a
8 TRCN0000007143 GCTCATAGTAACATCAACCAA pLKO.1 130 3UTR 100% 3.000 1.800 N TRIB2 n/a
9 TRCN0000362066 GACCTCAAGCTGCGGAAATTT pLKO_005 328 3UTR 100% 15.000 10.500 N Trib2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_027303.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03041 pDONR223 100% 27.5% None (many diffs) n/a
2 ccsbBroad304_03041 pLX_304 0% 27.5% V5 (many diffs) n/a
3 TRCN0000473109 GAAAACAGTATACCGCTGCCGACG pLX_317 47.5% 27.5% V5 (many diffs) n/a
4 ccsbBroadEn_15048 pDONR223 0% 27.5% None (many diffs) n/a
5 ccsbBroad304_15048 pLX_304 0% 27.5% V5 (many diffs) n/a
6 TRCN0000470263 GTATACCTCTGACCACTGATTAGC pLX_317 35.1% 27.5% V5 (many diffs) n/a
7 TRCN0000488723 TGCACACGGGTTATTCTTGGCGTG pLX_317 34.8% 27.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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