Construct: ORF TRCN0000488724
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019245.1_s317c1
- DNA Barcode:
- AAGTTCTTCTTGTCAAACGAACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRCP (27297)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488724
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27297 | CRCP | CGRP receptor component | NM_014478.5 | 100% | 100% | |
| 2 | human | 27297 | CRCP | CGRP receptor component | NM_001040647.1 | 77.7% | 77.7% | 45_46ins99 |
| 3 | human | 27297 | CRCP | CGRP receptor component | NM_001142414.1 | 65.9% | 65.5% | 1_78delinsA;81delT;123_124ins99 |
| 4 | human | 27297 | CRCP | CGRP receptor component | NM_001040648.1 | 47.7% | 45.9% | (many diffs) |
| 5 | human | 27297 | CRCP | CGRP receptor component | NR_024548.1 | 15.1% | 1_145del;189_310del;712_2925del | |
| 6 | mouse | 12909 | Crcp | calcitonin gene-related pep... | NM_007761.2 | 83.6% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 519
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggaa gtgaaggatg ccaattctgc gcttctcagt aactacgagg 121 tatttcagtt actaactgat cTGAAAGAGC AGCGTAAAGA AAGTGGAAAG AATAAACACA 181 GCTCTGGGCA ACAGAACTTG AACACTATCA CCTATGAAAC GTTAAAATAC ATATCAAAAA 241 CACCATGCAG GCACCAGAGT CCTGAAATTG TCAGAGAATT TCTCACAGCA TTGAAAAGCC 301 ACAAGTTGAC CAAAGCTGAG AAGCTCCAGC TGCTGAACCA CCGGCCTGTG ACTGCTGTGG 361 AGATCCAGCT GATGGTGGAA GAGAGTGAAG AGCGGCTCAC GGAGGAGCAG ATTGAAGCTC 421 TTCTCCACAC CGTCACCAGC ATTCTGCCTG CAGAGCCAGA GGCTGAGCAG AAGAAGAATA 481 CAAACAGCAA TGTGGCAATG GACGAAGAGG ACCCAGCAGA CCCAGCTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGAAAGTTC TTCTTGTCAA ACGAACTGAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt