Construct: ORF TRCN0000488738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021235.1_s317c1
- DNA Barcode:
- AATGCTGACTGTTTTCTGTTTCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TNKS2 (80351)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80351 | TNKS2 | tankyrase 2 | NM_025235.4 | 99.9% | 100% | 2361G>A |
| 2 | human | 80351 | TNKS2 | tankyrase 2 | XM_017016696.1 | 98.3% | 97.8% | (many diffs) |
| 3 | human | 80351 | TNKS2 | tankyrase 2 | XM_011540213.1 | 98.2% | 98.2% | 197_259del;2424G>A |
| 4 | human | 80351 | TNKS2 | tankyrase 2 | XM_005270185.4 | 96.6% | 96.1% | (many diffs) |
| 5 | human | 80351 | TNKS2 | tankyrase 2 | XM_017016699.1 | 89.9% | 87.1% | 0_1ins337;87_102del;2040G>A |
| 6 | human | 80351 | TNKS2 | tankyrase 2 | XM_017016697.1 | 88.3% | 85% | (many diffs) |
| 7 | human | 80351 | TNKS2 | tankyrase 2 | XM_017016698.2 | 88.3% | 85% | (many diffs) |
| 8 | human | 80351 | TNKS2 | tankyrase 2 | XM_017016700.2 | 81.9% | 81.4% | (many diffs) |
| 9 | human | 80351 | TNKS2 | tankyrase 2 | XM_011540215.2 | 59.5% | 59.6% | 0_1ins1413;948G>A |
| 10 | human | 80351 | TNKS2 | tankyrase 2 | XM_017016701.1 | 40.7% | 40.6% | (many diffs) |
| 11 | mouse | 74493 | Tnks2 | tankyrase, TRF1-interacting... | NM_001163635.1 | 90.7% | 97.2% | (many diffs) |
| 12 | mouse | 74493 | Tnks2 | tankyrase, TRF1-interacting... | XM_006527408.3 | 86.1% | 92.5% | (many diffs) |
| 13 | mouse | 74493 | Tnks2 | tankyrase, TRF1-interacting... | XM_006527409.3 | 85.8% | 91.8% | (many diffs) |
| 14 | mouse | 74493 | Tnks2 | tankyrase, TRF1-interacting... | XM_011247382.2 | 67.3% | 64% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 59
- ORF end:
- 3557
- ORF length:
- 3498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccat 61 gtcgggtcgc cgctgcgccg gcgggggagc ggcctgcgcg agcgccgcgg ccgaggccgt 121 ggagccggcc gcccgagagc tgttcgaggc gtgccgcaac ggggacgtgg aacgagtcaa 181 gaggctggtg acgcctgaga aggtgaacag ccgcgacacg gcgggcagga aatccacccc 241 gctgcacttc gccgcaggtt ttgggcggaa agacgtagtt gaatatttgc ttcagaatgg 301 tgcaaatgtc caagcacgtg atgatggggg ccttattcct cttcataatg catgctcttt 361 tggtcatgct gaagtagtca atctcctttt gcgacatggt gcagacccca atgctcgaga 421 taattggaat tatactcctc tccatgaagc tgcaattaaa ggaaagattg atgtttgcat 481 tgtgctgtta cagcatggag ctgagccaac catccgaaat acagatggaa ggacagcatt 541 ggatttagca gatccatctg ccaaagcagt gcttactggt gaatataaga aagatgaact 601 cttagaaagt gccaggagtg gcaatgaaga aaaaatgatg gctctactca caccattaaa 661 tgtcaactgc cacgcaagtg atggcagaaa gtcaactcca ttacatttgg cagcaggata 721 taacagagta aagattgtac agctgttact gcaacatgga gctgatgtcc atgctaaaga 781 taaaggtgat ctggtaccat tacacaatgc ctgttcttat ggtcattatg aagtaactga 841 acttttggtc aagcatggtg cctgtgtaaa tgcaatggac ttgtggcaat tcactcctct 901 tcatgaggca gcttctaaga acagggttga agtatgttct cttctcttaa gttatggtgc 961 agacccaaca ctgctcaatt gtcacaataa aagtgctata gacttggctc ccacaccaca 1021 gttaaaagaa agattagcat atgaatttaa aggccactcg ttgctgcaag ctgcacgaga 1081 agctgatgtt actcgaatca aaaaacatct ctctctggaa atggtgaatt tcaagcatcc 1141 tcaaacacat gaaacagcat tgcattgtgc tgctgcatct ccatatccca aaagaaagca 1201 aatatgtgaa ctgttgctaa gaaaaggagc aaacatcaat gaaaagacta aagaattctt 1261 gactcctctg cacgtggcat ctgagaaagc tcataatgat gttgttgaag tagtggtgaa 1321 acatgaagca aaggttaatg ctctggataa tcttggtcag acttctctac acagagctgc 1381 atattgtggt catctacaaa cctgccgcct actcctgagc tatgggtgtg atcctaacat 1441 tatatccctt cagggcttta ctgctttaca gatgggaaat gaaaatgtac agcaactcct 1501 ccaagagggt atctcattag gtaattcaga ggcagacaga caattgctgg aagctgcaaa 1561 ggctggagat gtcgaaactg taaaaaaact gtgtactgtt cagagtgtca actgcagaga 1621 cattgaaggg cgtcagtcta caccacttca ttttgcagct gggtataaca gagtgtccgt 1681 ggtggaatat ctgctacagc atggagctga tgtgcatgct aaagataaag gaggccttgt 1741 acctttgcac aatgcatgtt cttatggaca ttatgaagtt gcagaacttc ttgttaaaca 1801 tggagcagta gttaatgtag ctgatttatg gaaatttaca cctttacatg aagcagcagc 1861 aaaaggaaaa tatgaaattt gcaaacttct gctccagcat ggtgcagacc ctacaaaaaa 1921 aaacagggat ggaaatactc ctttggatct tgttaaagat ggagatacag atattcaaga 1981 tctgcttagg ggagatgcag ctttgctaga tgctgccaag aagggttgtt tagccagagt 2041 gaagaagttg tcttctcctg ataatgtaaa ttgccgcgat acccaaggca gacattcaac 2101 acctttacat ttagcagctg gttataataa tttagaagtt gcagagtatt tgttacaaca 2161 cggagctgat gtgaatgccc aagacaaagg aggacttatt cctttacata atgcagcatc 2221 ttacgggcat gtagatgtag cagctctact aataaagtat aatgcatgtg tcaatgccac 2281 ggacaaatgg gctttcacac ctttgcacga agcagcccaa aagggacgaa cacagctttg 2341 tgctttgttg ctagcccatg gagctgaccc gactcttaaa aatcaggaag gacaaacacc 2401 tttagattta gtttcagcag atgatgtcag cgctcttctg acagcagcca tgcccccatc 2461 tgctctgccc tcttgttaca agcctcaagt gctcaatggt gtgagaagcc caggagccac 2521 tgcagatgct ctctcttcag gtccatctag cccatcaagc ctttctgcag ccagcagtct 2581 tgacaactta tctgggagtt tttcagaact gtcttcagta gttagttcaa gtggaacaga 2641 gggtgcttcc agtttggaga aaaaggaggt tccaggagta gattttagca taactcaatt 2701 cgtaaggaat cttggacttg agcacctaat ggatatattt gagagagaac agatcacttt 2761 ggatgtatta gttgagatgg ggcacaagga gctgaaggag attggaatca atgcttatgg 2821 acataggcac aaactaatta aaggagtcga gagacttatc tccggacaac aaggtcttaa 2881 cccatattta actttgaaca cctctggtag tggaacaatt cttatagatc tgtctcctga 2941 tgataaagag tttcagtctg tggaggaaga gatgcaaagt acagttcgag agcacagaga 3001 tggaggtcat gcaggtggaa tcttcaacag atacaatatt ctcaagattc agaaggtttg 3061 taacaagaaa ctatgggaaa gatacactca ccggagaaaa gaagtttctg aagaaaacca 3121 caaccatgcc aatgaacgaa tgctatttca tgggTCTCCT TTTGTGAATG CAATTATCCA 3181 CAAAGGCTTT GATGAAAGGC ATGCGTACAT AGGTGGTATG TTTGGAGCTG GCATTTATTT 3241 TGCTGAAAAC TCTTCCAAAA GCAATCAATA TGTATATGGA ATTGGAGGAG GTACTGGGTG 3301 TCCAGTTCAC AAAGACAGAT CTTGTTACAT TTGCCACAGG CAGCTGCTCT TTTGCCGGGT 3361 AACCTTGGGA AAGTCTTTCC TGCAGTTCAG TGCAATGAAA ATGGCACATT CTCCTCCAGG 3421 TCATCACTCA GTCACTGGTA GGCCCAGTGT AAATGGCCTA GCATTAGCTG AATATGTTAT 3481 TTACAGAGGA GAACAGGCTT ATCCTGAGTA TTTAATTACT TACCAGATTA TGAGGCCTGA 3541 AGGTATGGTC GATGGATAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 3601 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 3661 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAATGC TGACTGTTTT 3721 CTGTTTCAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt