Construct: ORF TRCN0000488782
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020062.1_s317c1
- DNA Barcode:
- GAACCCAGATTCTCACCTGGCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A18 (83733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488782
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83733 | SLC25A18 | solute carrier family 25 me... | NM_001303484.1 | 99.8% | 99.6% | 945_946insG |
| 2 | human | 83733 | SLC25A18 | solute carrier family 25 me... | NM_031481.3 | 99.8% | 99.6% | 945_946insG |
| 3 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_011546151.2 | 99.8% | 99.6% | 945_946insG |
| 4 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_011546152.1 | 99.8% | 99.6% | 945_946insG |
| 5 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_011546153.3 | 99.8% | 99.6% | 945_946insG |
| 6 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_011546154.2 | 99.8% | 99.6% | 945_946insG |
| 7 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_017028968.1 | 99.8% | 99.6% | 945_946insG |
| 8 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_017028970.2 | 86.2% | 86% | 0_1ins129;816_817insG |
| 9 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_017028971.2 | 86.2% | 86% | 0_1ins129;816_817insG |
| 10 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_017028972.2 | 86.2% | 86% | 0_1ins129;816_817insG |
| 11 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_017028973.2 | 86.2% | 86% | 0_1ins129;816_817insG |
| 12 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_017028974.2 | 86.2% | 86% | 0_1ins129;816_817insG |
| 13 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_017028975.2 | 86.2% | 86% | 0_1ins129;816_817insG |
| 14 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_011546150.2 | 85.9% | 85.8% | 1_153del;1098_1099insG |
| 15 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XM_011546149.2 | 76.7% | 76.6% | 1_285del;1230_1231insG |
| 16 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XR_002958717.1 | 22.4% | (many diffs) | |
| 17 | human | 83733 | SLC25A18 | solute carrier family 25 me... | XR_951210.3 | 18.5% | 1_1496del;1783_1784ins119;2323_4345delinsG | |
| 18 | mouse | 71803 | Slc25a18 | solute carrier family 25 (m... | NM_001081048.2 | 83.8% | 86.2% | (many diffs) |
| 19 | mouse | 71803 | Slc25a18 | solute carrier family 25 (m... | XM_006506649.3 | 83.8% | 86.2% | (many diffs) |
| 20 | mouse | 71803 | Slc25a18 | solute carrier family 25 (m... | XM_006506650.3 | 83.8% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1017
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gacccaccag gatctgagca tcacagccaa actcatcaat ggaggtgtag 121 cagggctcgt gggggtgacc tgcgtgttcc ccatcgactt ggccaagact cgcctgcaga 181 accagcatgg gaaagccatg tacaaaggaa tgatcgactg cctgatgaag acggctcggg 241 cggagggctt cttcggcatg taccgagggg ctgcagtgaa cctcactctg gtcactccag 301 agaaggccat caagctggcg gccaacgact ttttccggcg gctgctcatg gaagatggga 361 tgcagcggaa cctgaagatg gagatgcttg ccgggtgtgg ggctgggatg tgccaggtcg 421 tggtgacctg tcccatggaa atgctcaaga ttcagctgca ggatgctgga cgcctggccg 481 tccatcatca gggctcggcc tcagcaccct ccacctccag gtcctacaca actggttcgg 541 cttccaccca caggcgcccc tctgccaccc tcattgcctg ggagctgctc cgcactcagg 601 gcctggctgg gctctacagg ggcctgggtg ccactctcct cagagacatt cctttctcca 661 tcatctactt cccactgttt gccaacctta acaaccTGGG GTTCAACGAG CTCGCCGGTA 721 AGGCGTCCTT TGCACATTCC TTCGTGTCAG GCTGTGTGGC AGGTTCCATA GCTGCGGTCG 781 CAGTGACGCC TCTAGATGTT CTGAAAACTC GAATCCAAAC CCTCAAGAAA GGCCTGGGCG 841 AGGACATGTA CAGTGGGATC ACCGACTGTG CCAGGAAACT CTGGATTCAG GAGGGACCAT 901 CTGCCTTCAT GAAAGGCGCT GGCTGCCGGG CACTGGTCAT AGCACCTCTC TTTGGGATTG 961 CTCAAGGGGT CTATTTTATT GGGATTGGAG AGCGCATCTT AAAGTGTTTT GACGACCCAG 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAG AACCCAGATT CTCACCTGGC CTAACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att