Construct: ORF TRCN0000488796
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021451.1_s317c1
- DNA Barcode:
- GAGGTACCTGTGTTACCTTATACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- P4HA3 (283208)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488796
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NM_182904.5 | 99.4% | 99.8% | 1198G>A;1632_1633insTGAAAGCT |
| 2 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NM_001288748.2 | 89.7% | 82.6% | (many diffs) |
| 3 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747836.1 | 85.5% | (many diffs) | |
| 4 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747837.1 | 83.6% | (many diffs) | |
| 5 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747838.1 | 77.6% | (many diffs) | |
| 6 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747839.1 | 71.4% | (many diffs) | |
| 7 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747840.1 | 65.1% | (many diffs) | |
| 8 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NR_110031.2 | 64.1% | (many diffs) | |
| 9 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | NM_177161.4 | 87.2% | 89.8% | (many diffs) |
| 10 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | XM_006507944.3 | 85.8% | 88.4% | (many diffs) |
| 11 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | XM_011241831.1 | 82.5% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1698
- ORF length:
- 1632
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatggg tcctggggcg cggctggcgg cgctgctggc ggtgctggcg ctcgggacag 121 gagacccaga aagggctgcg gctcggggcg acacgttctc ggcgctgacc agcgtggcgc 181 gcgccctggc gcccgagcgc cggctgctgg ggctgctgag gcggtacctg cgcggggagg 241 aggcgcggct gcgggacctg actagattct acgacaaggt actttctttg catgaggatt 301 caacaacccc tgtggctaac cctctgcttg catttactct catcaaacgc ctgcagtctg 361 actggaggaa tgtggtacat agtctggagg ccagtgagaa catccgagct ctgaaggatg 421 gctatgagaa ggtggagcaa gaccttccag cctttgagga ccttgaggga gcagcaaggg 481 ccctgatgcg gctgcaggac gtgtacatgc tcaatgtgaa aggcctggcc cgaggtgtct 541 ttcagagagt cactggctct gccatcactg acctgtacag ccccaaacgg ctcttttctc 601 tcacagggga tgactgcttc caagttggca aggtggccta tgacatgggg gattattacc 661 atgccattcc atggctggag gaggctgtca gtctcttccg aggatcttac ggagagtgga 721 agacagagga tgaggcaagt ctagaagatg ccttggatca cttggccttt gcttatttcc 781 gggcaggaaa tgtttcgtgt gccctcagcc tctctcggga gtttcttctc tacagcccag 841 ataataagag gatggccagg aatgtcttga aatatgaaag gctcttggca gagagcccca 901 accacgtggt agctgaggct gtcatccaga ggcccaatat accccacctg cagaccagag 961 acacctacga ggggctatgt cagaccctgg gttcccagcc cactctctac cagatcccta 1021 gcctctactg ttcctatgag accaattcca acgcctacct gctgctccag cccatccgga 1081 aggaggtcat ccacctggag ccctacattg ctctctacca tgacttcgtc agtgactcag 1141 aggctcagaa aattagagaa cttgcagaac catggctaca gaggtcagtg gtggcatcag 1201 gggagaagca gttacaagtg gagtaccgca tcagcaaaag tgcctggctg aaggacactg 1261 ttaacccaaa actggtgacc ctcaaccacc gcattgctgc cctcacaggc cttgatgtcc 1321 ggcctcccta tgcagagtat ctgcaggtgg tgaactatgg catcgGAGGA CACTATGAGC 1381 CTCACTTTGA CCATGCTACG TCACCAAGCA GCCCCCTCTA CAGAATGAAG TCAGGAAACC 1441 GAGTTGCAAC ATTTATGATC TATCTGAGCT CGGTGGAAGC TGGAGGAGCC ACAGCCTTCA 1501 TCTATGCCAA CCTCAGCGTG CCTGTGGTTA GGAATGCAGC ACTGTTTTGG TGGAACCTGC 1561 ACAGGAGTGG TGAAGGGGAC AGTGACACAC TTCATGCTGG CTGTCCTGTC CTGGTGGGAG 1621 ATAAGTGGGT GGCCAACAAG TGGATACATG AGTATGGACA GGAATTCCGC AGACCCTGCA 1681 GCTCCAGCCC TGAAGACTGA AAGCTTGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1741 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGCCCGT 1801 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAGGTACC 1861 TGTGTTACCT TATACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1921 aagatt