Construct: ORF TRCN0000488807
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020185.1_s317c1
- DNA Barcode:
- GTGTGGAACAGGCCGCTTTAATGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TKFC (26007)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488807
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26007 | TKFC | triokinase and FMN cyclase | NM_001351976.2 | 100% | 100% | |
| 2 | human | 26007 | TKFC | triokinase and FMN cyclase | NM_015533.4 | 100% | 100% | |
| 3 | human | 26007 | TKFC | triokinase and FMN cyclase | NM_001351978.1 | 93.6% | 91.2% | (many diffs) |
| 4 | human | 26007 | TKFC | triokinase and FMN cyclase | XM_017017519.2 | 92.5% | 89.6% | (many diffs) |
| 5 | human | 26007 | TKFC | triokinase and FMN cyclase | NM_001351979.2 | 92.5% | 91.3% | (many diffs) |
| 6 | human | 26007 | TKFC | triokinase and FMN cyclase | XM_017017517.1 | 92.4% | 92.4% | 1_139del;140_141insT |
| 7 | human | 26007 | TKFC | triokinase and FMN cyclase | NM_001351977.2 | 92% | 87.2% | (many diffs) |
| 8 | human | 26007 | TKFC | triokinase and FMN cyclase | XM_017017518.1 | 86.7% | 84.3% | (many diffs) |
| 9 | human | 26007 | TKFC | triokinase and FMN cyclase | XM_011544912.3 | 85.8% | 83% | (many diffs) |
| 10 | human | 26007 | TKFC | triokinase and FMN cyclase | XM_017017520.1 | 85.5% | 84.3% | (many diffs) |
| 11 | human | 26007 | TKFC | triokinase and FMN cyclase | NM_001351980.2 | 80.3% | 79.1% | (many diffs) |
| 12 | mouse | 225913 | Tkfc | triokinase, FMN cyclase | NM_145496.1 | 83.3% | 84.9% | (many diffs) |
| 13 | mouse | 225913 | Tkfc | triokinase, FMN cyclase | XM_006526942.2 | 83.3% | 84.9% | (many diffs) |
| 14 | mouse | 225913 | Tkfc | triokinase, FMN cyclase | XM_006526943.3 | 83.3% | 84.9% | (many diffs) |
| 15 | mouse | 225913 | Tkfc | triokinase, FMN cyclase | XM_006526944.3 | 83.3% | 84.9% | (many diffs) |
| 16 | mouse | 225913 | Tkfc | triokinase, FMN cyclase | XM_011247223.2 | 82.6% | 84.2% | (many diffs) |
| 17 | mouse | 225913 | Tkfc | triokinase, FMN cyclase | XM_006526941.3 | 76.4% | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1797
- ORF length:
- 1725
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacctcc aagaagctgg tgaactcggt ggctggctgt gctgatgacg 121 ctcttgctgg cctggtggcc tgcaacccca acctgcagct cctgcagggc caccgcgtgg 181 ccctccgttc tgacctggac agcctcaagg gccgggtggc actgctgtcg ggtgggggct 241 ctggccatga gcctgcccat gctggtttca tagggaaggg gatgctgact ggggtcatcg 301 cgggagctgt gttcacctcc ccggcagtgg gcagcatcct ggcagccatc agggccgtgg 361 cccaggccgg cacagtgggg acgctcctta tcgtgaagaa ctacactggg gatcggctca 421 acttcggcct ggcccgggag caggcccggg ctgaaggcat cccggtggag atggtggtga 481 ttggggacga cagcgccttc actgtcctga agaaggcagg ccggcggggg ctgtgcggca 541 cggtgcttat acacaaggtg gcaggtgctc tggctgaggc tggtgtgggg ctggaggaga 601 tcgcaaagca ggtgaacgtg gtcgccaagg ccatgggtac cctgggggtg agcttatcct 661 cctgcagcgt ccctggttcc aaacccacct tcgagctctc agccgacgag gtggagctgg 721 gcctggggat ccacggggaa gctggtgtgc gccggataaa gatggcaacc gccgatgaga 781 ttgtgaaact catgctcgac cacatgacaa acaccaccaa cgcgtcccat gtgcctgtgc 841 agcccggctc ctcagttgtg atgatggtca acaacctggg tggcctgtca ttcctggaac 901 tgggcatcat agccgacgct accgtccgct ccctggaggg ccgcggggtg aagattgccc 961 gtgccctggt gggcaccttc atgtcagcac tggagatgcc tggcatttct ctcaccctcc 1021 tgctggtgga tgagcctctc ctgaaactga tagatgctga aaccactgca gcagcctggc 1081 ctaacgtggc tgcagtctcc attactgggc ggaagcggag ccgggtagcc cctgccgagc 1141 cccaggaggc ccctgattcc actgctgcag gaggctcagc ctcgaagcgg atggcgctgg 1201 tgctggaacg ggtgtgcagc actctcctgg gcctggagga acacctgaat gccctggacc 1261 gggctgctgg tgacggcgac tgtggcacca cccacagccg tgcggccaga gcaatccagg 1321 agtggctgaa ggagggccca ccccctgcca gccctgccca gctgctctcc aagttgtctg 1381 tcctgctcct ggagaagatg ggaggctcat ctggggcgct ctatggcctg ttcctgactg 1441 cggctgcaca gccccTGAAG GCCAAGACCA GCCTCCCAGC CTGGTCTGCT GCCATGGATG 1501 CCGGCCTGGA AGCCATGCAG AAGTATGGCA AGGCTGCTCC AGGGGACAGG ACTATGCTGG 1561 ATTCTCTGTG GGCAGCGGGG CAGGAGCTCC AAGCCTGGAA GAGCCCAGGA GCTGATCTGT 1621 TACAAGTCCT GACCAAAGCA GTCAAGAGTG CCGAAGCTGC AGCCGAGGCC ACCAAGAATA 1681 TGGAAGCTGG AGCCGGAAGA GCCAGTTATA TCAGCTCAGC ACGGCTGGAG CAGCCAGACC 1741 CCGGGGCGGT GGCAGCTGCT GCCATCCTCC GGGCCATCTT GGAGGTCTTG CAGAGCTGAG 1801 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1861 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1921 TTTATATATC TTGTGGAAAG GACGAGTGTG GAACAGGCCG CTTTAATGGA CGCGTTAAGT 1981 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt