Transcript: Human NM_015533.4

Homo sapiens triokinase and FMN cyclase (TKFC), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
TKFC (26007)
Length:
4678
CDS:
212..1939

Additional Resources:

NCBI RefSeq record:
NM_015533.4
NBCI Gene record:
TKFC (26007)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146826 TATCGTGAAGAACTACACTG pXPR_003 GGG 334 19% 5 1.0009 TKFC TKFC 75784
2 BRDN0001148963 CAGGGAGCGGACGGTAGCGT pXPR_003 CGG 847 49% 10 0.6106 TKFC TKFC 75785
3 BRDN0001149092 GGTCAGAACGGAGGGCCACG pXPR_003 CGG 107 6% 3 0.2295 TKFC TKFC 75786
4 BRDN0001144729 GATCGCAAAGCAGGTGAACG pXPR_003 TGG 544 31% 6 -0.3834 TKFC TKFC 75783
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_015533.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000078622 CCTCTCCTGAAACTGATAGAT pLKO.1 1175 CDS 100% 5.625 3.938 N TKFC n/a
2 TRCN0000300769 CCTCTCCTGAAACTGATAGAT pLKO_005 1175 CDS 100% 5.625 3.938 N TKFC n/a
3 TRCN0000078618 CCCTCTAAGTTGAGCAGGAAA pLKO.1 2056 3UTR 100% 4.950 3.465 N TKFC n/a
4 TRCN0000300847 CCCTCTAAGTTGAGCAGGAAA pLKO_005 2056 3UTR 100% 4.950 3.465 N TKFC n/a
5 TRCN0000078621 CTGTTACAAGTCCTGACCAAA pLKO.1 1757 CDS 100% 4.950 3.465 N TKFC n/a
6 TRCN0000078620 GCTCCTTATCGTGAAGAACTA pLKO.1 523 CDS 100% 4.950 3.465 N TKFC n/a
7 TRCN0000300802 GCTCCTTATCGTGAAGAACTA pLKO_005 523 CDS 100% 4.950 3.465 N TKFC n/a
8 TRCN0000078619 CCACATGACAAACACCACCAA pLKO.1 940 CDS 100% 2.640 1.848 N TKFC n/a
9 TRCN0000300846 CCACATGACAAACACCACCAA pLKO_005 940 CDS 100% 2.640 1.848 N TKFC n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_015533.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15031 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15031 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000471648 CAATCTAAACGGTAAATCGTACAT pLX_317 24.1% 100% 100% V5 n/a
4 TRCN0000488807 GTGTGGAACAGGCCGCTTTAATGG pLX_317 20.2% 100% 100% V5 (not translated due to prior stop codon) n/a
5 ccsbBroadEn_14101 pDONR223 100% 99.9% 99.4% None 1719delG n/a
6 ccsbBroad304_14101 pLX_304 0% 99.9% 99.4% V5 (not translated due to frame shift) 1719delG n/a
7 TRCN0000480040 CACTACCGCCGCATCTCTTGGGCA pLX_317 6.4% 99.9% 99.4% V5 (not translated due to frame shift) 1719delG n/a
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