Construct: ORF TRCN0000488904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020311.1_s317c1
- DNA Barcode:
- TAAACTCATCTGACCCGGGACTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRK5 (2869)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488904
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2869 | GRK5 | G protein-coupled receptor ... | NM_005308.3 | 100% | 100% | |
| 2 | human | 2869 | GRK5 | G protein-coupled receptor ... | XM_005269707.2 | 94.9% | 94.9% | 966_967ins90 |
| 3 | human | 2869 | GRK5 | G protein-coupled receptor ... | XM_005269708.1 | 94.5% | 94.5% | 50_51ins96 |
| 4 | mouse | 14773 | Grk5 | G protein-coupled receptor ... | NM_018869.3 | 88.4% | 95.2% | (many diffs) |
| 5 | mouse | 14773 | Grk5 | G protein-coupled receptor ... | XM_006526697.3 | 83.2% | 89.8% | (many diffs) |
| 6 | mouse | 14773 | Grk5 | G protein-coupled receptor ... | XM_011247149.2 | 80.6% | 86.9% | (many diffs) |
| 7 | mouse | 14773 | Grk5 | G protein-coupled receptor ... | XM_017318066.1 | 74.2% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1842
- ORF length:
- 1770
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagctg gaaaacatcg tggccaacac ggtcttgctg aaagccaggg 121 aagggggcgg aggaaagcgc aaagggaaaa gcaagaagtg gaaagaaatc ctgaagttcc 181 ctcacattag ccagtgtgaa gacctccgaa ggaccataga cagagattac tgcagtttat 241 gtgacaagca gccaatcggg aggctgcttt tccggcagtt ttgtgaaacc aggcctgggc 301 tggagtgtta cattcagttc ctggactccg tggcagaata tgaagttact ccagatgaaa 361 aactgggaga gaaagggaag gaaattatga ccaagtacct caccccaaag tcccctgttt 421 tcatagccca agttggccaa gacctggtct cccagacgga ggagaagctc ctacagaagc 481 cgtgcaaaga actcttttct gcctgtgcac agtctgtcca cgagtacctg aggggagaac 541 cattccacga atatctggac agcatgtttt ttgaccgctt tctccagtgg aagtggttgg 601 aaaggcaacc ggtgaccaaa aacactttca ggcagtatcg agtgctagga aaagggggct 661 tcggggaggt ctgtgcctgc caggttcggg ccacgggtaa aatgtatgcc tgcaagcgct 721 tggagaagaa gaggatcaaa aagaggaaag gggagtccat ggccctcaat gagaagcaga 781 tcctcgagaa ggtcaacagt cagtttgtgg tcaacctggc ctatgcctac gagaccaagg 841 atgcactgtg cttggtcctg accatcatga atgggggtga cctgaagttc cacatctaca 901 acatgggcaa ccctggcttc gaggaggagc gggccttgtt ttatgcggca gagatcctct 961 gcggcttaga agacctccac cgtgagaaca ccgtctaccg agatctgaaa cctgaaaaca 1021 tcctgttaga tgattatggc cacattagga tctcagacct gggcttggct gtgaagatcc 1081 ccgagggaga cctgatccgc ggccgggtgg gcactgttgg ctacatggct ccagaggtcc 1141 tgaacaacca gaggtacggc ctgagccccg actactgggg ccttggctgc ctcatctatg 1201 agatgatcga gggccagtcg ccgttccgcg gccgcaagga gaaggtgaag cgggaggagg 1261 tggaccgccg ggtcctggag acggaggagg tgtactccca caagttctcc gaggaggcca 1321 agtccatctg caagatgctg ctcacgaaag atgcgaagca gaggctgggc tgccaggagg 1381 agggggctgc agaggtcaag agacacccct tcttcaggaa catgaacttc aagcgcttag 1441 aagccgggat gttggaccct cccttcgttc cagacccccg cgctgtgtac tgtaaggacg 1501 tgctggacat cgagcagttc tccactgtga agggcgtcaa tctggaccac acagacgacg 1561 acttctactc caagttctcc acgggctctg tgtccatCCC ATGGCAAAAC GAGATGATAG 1621 AAACAGAATG CTTTAAGGAG CTGAACGTGT TTGGACCTAA TGGTACCCTC CCGCCAGATC 1681 TGAACAGAAA CCACCCTCCG GAACCGCCCA AGAAAGGGCT GCTCCAGAGA CTCTTCAAGC 1741 GGCAGCATCA GAACAATTCC AAGAGTTCGC CCAGCTCCAA GACCAGTTTT AACCACCACA 1801 TAAACTCAAA CCATGTCAGC TCGAACTCCA CCGGAAGCAG CTGAGACCCA GCTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA TAAACTCATC TGACCCGGGA CTTCACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt