Construct: ORF TRCN0000488904

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020311.1_s317c1
DNA Barcode:
TAAACTCATCTGACCCGGGACTTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GRK5 (2869)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488904
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2869 GRK5 G protein-coupled receptor ... NM_005308.3 100% 100%
2 human 2869 GRK5 G protein-coupled receptor ... XM_005269707.2 94.9% 94.9% 966_967ins90
3 human 2869 GRK5 G protein-coupled receptor ... XM_005269708.1 94.5% 94.5% 50_51ins96
4 mouse 14773 Grk5 G protein-coupled receptor ... NM_018869.3 88.4% 95.2% (many diffs)
5 mouse 14773 Grk5 G protein-coupled receptor ... XM_006526697.3 83.2% 89.8% (many diffs)
6 mouse 14773 Grk5 G protein-coupled receptor ... XM_011247149.2 80.6% 86.9% (many diffs)
7 mouse 14773 Grk5 G protein-coupled receptor ... XM_017318066.1 74.2% 78.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1842
ORF length:
1770
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggagctg gaaaacatcg tggccaacac ggtcttgctg aaagccaggg
121aagggggcgg aggaaagcgc aaagggaaaa gcaagaagtg gaaagaaatc ctgaagttcc
181ctcacattag ccagtgtgaa gacctccgaa ggaccataga cagagattac tgcagtttat
241gtgacaagca gccaatcggg aggctgcttt tccggcagtt ttgtgaaacc aggcctgggc
301tggagtgtta cattcagttc ctggactccg tggcagaata tgaagttact ccagatgaaa
361aactgggaga gaaagggaag gaaattatga ccaagtacct caccccaaag tcccctgttt
421tcatagccca agttggccaa gacctggtct cccagacgga ggagaagctc ctacagaagc
481cgtgcaaaga actcttttct gcctgtgcac agtctgtcca cgagtacctg aggggagaac
541cattccacga atatctggac agcatgtttt ttgaccgctt tctccagtgg aagtggttgg
601aaaggcaacc ggtgaccaaa aacactttca ggcagtatcg agtgctagga aaagggggct
661tcggggaggt ctgtgcctgc caggttcggg ccacgggtaa aatgtatgcc tgcaagcgct
721tggagaagaa gaggatcaaa aagaggaaag gggagtccat ggccctcaat gagaagcaga
781tcctcgagaa ggtcaacagt cagtttgtgg tcaacctggc ctatgcctac gagaccaagg
841atgcactgtg cttggtcctg accatcatga atgggggtga cctgaagttc cacatctaca
901acatgggcaa ccctggcttc gaggaggagc gggccttgtt ttatgcggca gagatcctct
961gcggcttaga agacctccac cgtgagaaca ccgtctaccg agatctgaaa cctgaaaaca
1021tcctgttaga tgattatggc cacattagga tctcagacct gggcttggct gtgaagatcc
1081ccgagggaga cctgatccgc ggccgggtgg gcactgttgg ctacatggct ccagaggtcc
1141tgaacaacca gaggtacggc ctgagccccg actactgggg ccttggctgc ctcatctatg
1201agatgatcga gggccagtcg ccgttccgcg gccgcaagga gaaggtgaag cgggaggagg
1261tggaccgccg ggtcctggag acggaggagg tgtactccca caagttctcc gaggaggcca
1321agtccatctg caagatgctg ctcacgaaag atgcgaagca gaggctgggc tgccaggagg
1381agggggctgc agaggtcaag agacacccct tcttcaggaa catgaacttc aagcgcttag
1441aagccgggat gttggaccct cccttcgttc cagacccccg cgctgtgtac tgtaaggacg
1501tgctggacat cgagcagttc tccactgtga agggcgtcaa tctggaccac acagacgacg
1561acttctactc caagttctcc acgggctctg tgtccatCCC ATGGCAAAAC GAGATGATAG
1621AAACAGAATG CTTTAAGGAG CTGAACGTGT TTGGACCTAA TGGTACCCTC CCGCCAGATC
1681TGAACAGAAA CCACCCTCCG GAACCGCCCA AGAAAGGGCT GCTCCAGAGA CTCTTCAAGC
1741GGCAGCATCA GAACAATTCC AAGAGTTCGC CCAGCTCCAA GACCAGTTTT AACCACCACA
1801TAAACTCAAA CCATGTCAGC TCGAACTCCA CCGGAAGCAG CTGAGACCCA GCTTTCTTGT
1861ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1921AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1981GAAAGGACGA TAAACTCATC TGACCCGGGA CTTCACGCGT TAAGTCgaca atcaacctct
2041ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.