Construct: ORF TRCN0000488907
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019288.1_s317c1
- DNA Barcode:
- AGGCGTGCCGTTCCACTCACTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPR1 (4881)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488907
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | NM_000906.4 | 99.9% | 100% | 2679A>G |
| 2 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | XM_005245218.2 | 97.5% | 97.5% | 1680_1681ins78;2601A>G |
| 3 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | XM_017001374.2 | 66.2% | 65.9% | (many diffs) |
| 4 | mouse | 18160 | Npr1 | natriuretic peptide receptor 1 | NM_008727.5 | 86.4% | 90.8% | (many diffs) |
| 5 | mouse | 18160 | Npr1 | natriuretic peptide receptor 1 | XR_375494.1 | 49.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3258
- ORF length:
- 3183
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgccg gggccccggc gccccgctgg ctcccgcctg cgcctgctcc 121 tgctcctgct gctgccgccg ctgctgctgc tgctccgggg cagccacgcg ggcaacctga 181 cggtagccgt ggtactgccg ctggccaata cctcgtaccc ctggtcgtgg gcgcgcgtgg 241 gacccgccgt ggagctggcc ctggcccagg tgaaggcgcg ccccgacttg ctgccgggct 301 ggacggtccg cacggtgctg ggcagcagcg aaaacgcgct gggcgtctgc tccgacaccg 361 cagcgcccct ggccgcggtg gacctcaagt gggagcacaa ccccgctgtg ttcctgggcc 421 ccggctgcgt gtacgccgcc gccccagtgg ggcgcttcac cgcgcactgg cgggtcccgc 481 tgctgaccgc cggcgccccg gcgctgggct tcggtgtcaa ggacgagtat gcgctgacca 541 cccgcgcggg gcccagctac gccaagctgg gggacttcgt ggcggcgctg caccgacggc 601 tgggctggga gcgccaagcg ctcatgctct acgcctaccg gccgggtgac gaagagcact 661 gcttcttcct cgtggagggg ctgttcatgc gggtccgcga ccgcctcaat attacggtgg 721 accacctgga gttcgccgag gacgacctca gccactacac caggctgctg cggaccatgc 781 cgcgcaaagg ccgagttatc tacatctgca gctcccctga tgccttcaga accctcatgc 841 tcctggccct ggaagctggc ttgtgtgggg aggactacgt tttcttccac ctggatatct 901 ttgggcaaag cctgcaaggt ggacagggcc ctgctccccg caggccctgg gagagagggg 961 atgggcagga tgtcagtgcc cgccaggcct ttcaggctgc caaaatcatt acatataaag 1021 acccagataa tcccgagtac ttggaattcc tgaagcagtt aaaacacctg gcctatgagc 1081 agttcaactt caccatggag gatggcctgg tgaacaccat cccagcatcc ttccacgacg 1141 ggctcctgct ctatatccag gcagtgacgg agactctggc acatggggga actgttactg 1201 atggggagaa catcactcag cggatgtgga accgaagctt tcaaggtgtg acaggatacc 1261 tgaaaattga tagcagtggc gatcgggaaa cagacttctc cctctgggat atggatcccg 1321 agaatggtgc cttcagggtt gtactgaact acaatgggac ttcccaagag ctggtggctg 1381 tgtcggggcg caaactgaac tggcccctgg ggtaccctcc tcctgacatc cccaaatgtg 1441 gctttgacaa cgaagaccca gcatgcaacc aagatcacct ttccaccctg gaggtgctgg 1501 ctttggtggg cagcctctcc ttgctcggca ttctgattgt ctccttcttc atatacagga 1561 agatgcagct ggagaaggaa ctggcctcgg agctgtggcg ggtgcgctgg gaggacgttg 1621 agcccagtag ccttgagagg cacctgcgga gtgcaggcag ccggctgacc ctgagcggga 1681 gaggctccaa ttacggctcc ctgctaacca cagagggcca gttccaagtc tttgccaaga 1741 cagcatatta taagggcaac ctcgtggctg tgaaacgtgt gaaccgtaaa cgcattgagc 1801 tgacacgaaa agtcctgttt gaactgaagc atatgcggga tgtgcagaat gaacacctga 1861 ccaggtttgt gggagcctgc accgaccccc ccaatatctg catcctcaca gagtactgtc 1921 cccgtgggag cctgcaggac attctggaga atgagagcat caccctggac tggatgttcc 1981 ggtactcact caccaatgac atcgtcaagg gcatgctgtt tctacacaat ggggctatct 2041 gttcccatgg gaacctcaag tcatccaact gcgtggtaga tgggcgcttt gtgctcaaga 2101 tcaccgacta tgggctggag agcttcaggg acctggaccc agagcaagga cacaccgttt 2161 atgccaaaaa gctgtggacg gcccctgagc tcctgcgaat ggcttcaccc cctgtgcggg 2221 gctcccaggc tggtgacgta tacagctttg ggatcatcct tcaggagatt gccctgagga 2281 gtggggtctt ccacgtggaa ggtttggacc tgagccccaa agagatcatc gagcgggtga 2341 ctcggggtga gcagcccccc ttccggccct ccctggccct gcagagtcac ctggaggagt 2401 tggggctgct catgcagcgg tgctgggctg aggacccaca ggagaggcca ccattccagc 2461 agatccgcct gacgttgcgc aaatttaaca gggagaacag cagcaacatc ctggacaacc 2521 tgctgtcccg catggagcag tacgcgaaca atctggagga actggtggag gagcggaccc 2581 aggcatacct ggaggagaag cgcaaggctg aggccctgct ctaccagatc ctgcctcact 2641 cagtggctga gcagctgaag cgtggggaga cggtgcaggc cgaagccttt gacagtgtta 2701 ccatctactt cagtgacatt gtgggtttca cagcgctgtc ggcggagagc acgcccatgc 2761 aggtggtgac cctgctcaat gacctgtaca cttgctttga tgctgtcata gacaactttg 2821 atgtgtacaa ggtggagaca attggcgatg cctacatggt ggtgtcaggg ctccctgtgc 2881 ggaacgggcg gctacacgcc TGCGAGGTAG CCCGCATGGC CCTGGCACTG CTGGATGCTG 2941 TGCGCTCCTT CCGAATCCGC CACCGGCCCC AGGAGCAGCT GCGCTTGCGC ATTGGCATCC 3001 ACACAGGACC TGTGTGTGCT GGAGTGGTGG GACTGAAGAT GCCCCGTTAC TGTCTCTTTG 3061 GGGATACAGT CAACACAGCC TCAAGAATGG AGTCTAATGG GGAAGCCCTG AAGATCCACT 3121 TGTCTTCTGA GACCAAGGCT GTCCTGGAGG AGTTTGGTGG TTTCGAGCTG GAGCTTCGAG 3181 GGGATGTAGA AATGAAGGGC AAAGGCAAGG TTCGGACCTA CTGGCTCCTT GGGGAGAGGG 3241 GGAGTAGCAC CCGAGGCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3301 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3361 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGGCGTG CCGTTCCACT 3421 CACTATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt