Construct: ORF TRCN0000488914

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019729.1_s317c1
DNA Barcode:
TCCGATTCCCGCTAGAGTGGAAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKCE (5581)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488914
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5581 PRKCE protein kinase C epsilon NM_005400.3 99.9% 99.8% 2211_2212insG
2 human 5581 PRKCE protein kinase C epsilon XM_005264428.1 99.9% 99.8% 2211_2212insG
3 human 5581 PRKCE protein kinase C epsilon XM_011532971.3 86% 84% (many diffs)
4 human 5581 PRKCE protein kinase C epsilon XM_011532975.3 86% 84% (many diffs)
5 human 5581 PRKCE protein kinase C epsilon XM_017004486.2 86% 84% (many diffs)
6 human 5581 PRKCE protein kinase C epsilon XM_011532978.2 84.2% 84.1% 1_1delAins345;4_5delCA;1869_1870insG
7 human 5581 PRKCE protein kinase C epsilon XM_017004487.2 84.2% 84.1% 1_1delAins345;4_5delCA;1869_1870insG
8 human 5581 PRKCE protein kinase C epsilon XM_017004488.2 84.2% 84.1% 1_1delAins345;4_5delCA;1869_1870insG
9 human 5581 PRKCE protein kinase C epsilon XM_011532980.3 79.6% 79.5% 0_1ins450;1761_1762insG
10 human 5581 PRKCE protein kinase C epsilon XM_011532981.3 79.6% 79.5% 0_1ins450;1761_1762insG
11 human 5581 PRKCE protein kinase C epsilon XM_017004489.2 79.6% 79.5% 0_1ins450;1761_1762insG
12 human 5581 PRKCE protein kinase C epsilon XM_017004490.2 79.6% 79.5% 0_1ins450;1761_1762insG
13 human 5581 PRKCE protein kinase C epsilon XM_017004491.2 79.6% 79.5% 0_1ins450;1761_1762insG
14 human 5581 PRKCE protein kinase C epsilon XM_024452991.1 79.6% 79.5% 0_1ins450;1761_1762insG
15 human 5581 PRKCE protein kinase C epsilon XM_024452992.1 79.6% 79.5% 0_1ins450;1761_1762insG
16 human 5581 PRKCE protein kinase C epsilon XM_011532982.2 67.4% 67.3% 0_1ins720;1491_1492insG
17 human 5581 PRKCE protein kinase C epsilon XM_005264431.4 65.9% 65.1% (many diffs)
18 human 5581 PRKCE protein kinase C epsilon XM_017004492.2 65.1% 64.9% (many diffs)
19 human 5581 PRKCE protein kinase C epsilon XM_006712050.3 62.3% 62.3% 0_1ins831;1380_1381insG
20 human 5581 PRKCE protein kinase C epsilon XM_011532983.2 58.1% 57.1% (many diffs)
21 human 5581 PRKCE protein kinase C epsilon XR_939695.2 54.7% (many diffs)
22 human 5581 PRKCE protein kinase C epsilon XR_001738846.2 18% (many diffs)
23 mouse 18754 Prkce protein kinase C, epsilon NM_011104.3 91% 97.8% (many diffs)
24 mouse 18754 Prkce protein kinase C, epsilon XM_017317328.1 85.6% 91.5% (many diffs)
25 mouse 18754 Prkce protein kinase C, epsilon XM_006523833.2 74.2% 79.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2286
ORF length:
2214
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtagtg ttcaatggcc ttcttaagat caaaatctgc gaggccgtga
121gcttgaagcc cacagcctgg tcgctgcgcc atgcggtggg accccggccg cagactttcc
181ttctcgaccc ctacattgcc ctcaatgtgg acgactcgcg catcggccaa acggccacca
241agcagaagac caacagcccg gcctggcacg acgagttcgt caccgatgtg tgcaacggac
301gcaagatcga gctggctgtc tttcacgatg cccccatagg ctacgacgac ttcgtggcca
361actgcaccat ccagtttgag gagctgctgc agaacgggag ccgccacttc gaggactgga
421ttgatctgga gccagaagga agagtgtatg tgatcatcga tctctcaggg tcgtcgggtg
481aagcccctaa agacaatgaa gagcgtgtgt tcagggaacg catgcggccg aggaagcggc
541agggggccgt caggcgcagg gtccatcagg tcaacggcca caagttcatg gccacctatc
601ttcggcagcc cacctactgc tcccattgca gagacttcat ctggggtgtc ataggaaagc
661agggatacca gtgtcaagtc tgcacctgcg tggtccacaa gcggtgccac gagctcataa
721tcacaaagtg tgctgggtta aagaagcagg agacccccga ccaggtgggc tcccagcggt
781tcagcgtcaa catgccccac aagttcggta tccacaacta caaggtccct accttctgcg
841atcactgtgg gtccctgctc tggggactct tgcggcaggg tttgcagtgt aaagtctgca
901aaatgaatgt tcaccgtcga tgtgagacca acgtggctcc caactgtgga gtggatgcca
961gaggaatcgc caaagtactg gccgacctgg gcgttacccc agacaaaatc accaacagcg
1021gccagagaag gaaaaagctc attgctggtg ccgagtcccc gcagcctgct tctggaagct
1081caccatctga ggaagatcga tccaagtcag cacccacctc cccttgtgac caggaaataa
1141aagaacttga gaacaacatt cggaaagcct tgtcatttga caaccgagga gaggagcacc
1201gggcagcatc gtctcctgat ggccagctga tgagccccgg tgagaatggc gaagtccggc
1261aaggccaggc caagcgcctg ggcctggatg agttcaactt catcaaggtg ttgggcaaag
1321gcagctttgg caaggtcatg ttggcagaac tcaagggcaa agatgaagta tatgctgtga
1381aggtcttaaa gaaggacgtc atccttcagg atgatgacgt ggactgcaca atgacagaga
1441agaggatttt ggctctggca cggaaacacc cgtaccttac ccaactctac tgctgcttcc
1501agaccaagga ccgcctcttt ttcgtcatgg aatatgtaaa tggtggagac ctcatgtttc
1561agattcagcg ctcccgaaaa ttcgacgagc ctcgttcacg gttctatgct gcagaggtca
1621catcggccct catgttcctc caccagcatg gagtcatcta cagggatttg aaactggaca
1681acatccttct ggatgcagaa ggtcactgca agctggctga cttcgggatg tgcaaggaag
1741ggattctgaa tggtgtgacg accaccacgt tctgtgggac tcctgactac atagctcctg
1801agatcctgca ggagttggag tatggcccct ccgtggactg gtgggccctg ggggtgctga
1861tgtacgagat gatggctgga cagcctccct ttgaggccga caatgaggac gacctatttg
1921agtccatcct ccatgacgac gtgctgtacc cagtctggcT CAGCAAGGAG GCTGTCAGCA
1981TCTTGAAAGC TTTCATGACG AAGAATCCCC ACAAGCGCCT GGGCTGTGTG GCATCGCAGA
2041ATGGCGAGGA CGCCATCAAG CAGCACCCAT TCTTCAAAGA GATTGACTGG GTGCTCCTGG
2101AGCAGAAGAA GATCAAGCCA CCCTTCAAAC CACGCATTAA AACCAAAAGA GACGTCAATA
2161ATTTTGACCA AGACTTTACC CGGGAAGAGC CGGTACTCAC CCTTGTGGAC GAAGCAATTG
2221TAAAGCAGAT CAACCAGGAG GAATTCAAAG GTTTCTCCTA CTTTGGTGAA GACCTGATGC
2281CCGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2341TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2401GGCTTTATAT ATCTTGTGGA AAGGACGATC CGATTCCCGC TAGAGTGGAA AGACGCGTTA
2461AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)