Construct: ORF TRCN0000488915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019641.1_s317c1
- DNA Barcode:
- ATCAAATATCTTTTAGGCTAAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AURKB (9212)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9212 | AURKB | aurora kinase B | NM_001313950.2 | 99.8% | 99.7% | 893T>C;1017C>T |
| 2 | human | 9212 | AURKB | aurora kinase B | NM_004217.4 | 99.8% | 99.7% | 893T>C;1017C>T |
| 3 | human | 9212 | AURKB | aurora kinase B | NM_001284526.2 | 99.5% | 99.4% | 207_209delCAG;896T>C;1020C>T |
| 4 | human | 9212 | AURKB | aurora kinase B | NM_001313953.3 | 88.7% | 86.9% | (many diffs) |
| 5 | human | 9212 | AURKB | aurora kinase B | NM_001256834.3 | 87.8% | 87.7% | 0_1ins123;770T>C;894C>T |
| 6 | human | 9212 | AURKB | aurora kinase B | NM_001313951.1 | 87.8% | 87.7% | 0_1ins123;770T>C;894C>T |
| 7 | human | 9212 | AURKB | aurora kinase B | XM_011524072.3 | 87.8% | 87.7% | 0_1ins123;770T>C;894C>T |
| 8 | human | 9212 | AURKB | aurora kinase B | XM_017025307.2 | 87.8% | 87.7% | 0_1ins123;770T>C;894C>T |
| 9 | human | 9212 | AURKB | aurora kinase B | NM_001313952.2 | 87.6% | 87.5% | (many diffs) |
| 10 | human | 9212 | AURKB | aurora kinase B | XM_017025308.2 | 76.9% | 75% | (many diffs) |
| 11 | human | 9212 | AURKB | aurora kinase B | NR_132731.1 | 74.4% | (many diffs) | |
| 12 | human | 9212 | AURKB | aurora kinase B | NR_132730.2 | 71.4% | (many diffs) | |
| 13 | human | 9212 | AURKB | aurora kinase B | NM_001313954.2 | 54.1% | 48.9% | (many diffs) |
| 14 | human | 9212 | AURKB | aurora kinase B | XM_017025309.1 | 54.1% | 48.9% | (many diffs) |
| 15 | human | 9212 | AURKB | aurora kinase B | XM_017025310.1 | 54.1% | 48.9% | (many diffs) |
| 16 | human | 9212 | AURKB | aurora kinase B | XM_017025311.1 | 54.1% | 48.9% | (many diffs) |
| 17 | human | 9212 | AURKB | aurora kinase B | NM_001313955.2 | 50% | 47.2% | (many diffs) |
| 18 | mouse | 20877 | Aurkb | aurora kinase B | NM_011496.2 | 81.3% | 82.9% | (many diffs) |
| 19 | mouse | 20877 | Aurkb | aurora kinase B | XM_006532725.3 | 53.5% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 1125
- ORF length:
- 1032
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgaa ttctctctaa ggatggccca gaaggagaac tcctacccct 121 ggccctacgg ccgacagacg gctccatctg gcctgagcac cctgccccag cgagtcctcc 181 ggaaagagcc tgtcacccca tctgcacttg tcctcatgag ccgctccaat gtccagccca 241 cagctgcccc tggccagaag gtgatggaga atagcagtgg gacacccgac atcttaacgc 301 ggcacttcac aattgatgac tttgagattg ggcgtcctct gggcaaaggc aagtttggaa 361 acgtgtactt ggctcgggag aagaaaagcc atttcatcgt ggcgctcaag gtcctcttca 421 agtcccagat agagaaggag ggcgtggagc atcagctgcg cagagagatc gaaatccagg 481 cccacctgca ccatcccaac atcctgcgtc tctacaacta tttttatgac cggaggagga 541 tctacttgat tctagagtat gccccccgcg gggagctcta caaggagctg cagaagagct 601 gcacatttga cgagcagcga acagccacga tcatggagga gttggcagat gctctaatgt 661 actgccatgg gaagaaggtg attcacagag acataaagcc agaaaatctg ctcttagggc 721 tcaagggaga gctgaagatt gctgacttcg gctggtctgt gcatgcgccc tcccTGAGGA 781 GGAAGACAAT GTGTGGCACC CTGGACTACC TGCCCCCAGA GATGATTGAG GGGCGCATGC 841 ACAATGAGAA GGTGGATCTG TGGTGCATTG GAGTGCTTTG CTATGAGCTG CTGGTGGGGA 901 ACCCACCCTT TGAGAGTGCA TCACACAACG AGACCTATCG CCGCATCGTC AAGGTGGACC 961 TAAAGTTCCC CGCTTCCGTG CCCACGGGAG CCCAGGACCT CATCTCCAAA CTGCTCAGGC 1021 ATAACCCCTC GGAACGGCTG CCCCTGGCCC AGGTCTCAGC CCACCCTTGG GTCCGGGCCA 1081 ACTCTCGGAG GGTGCTGCCT CCCTCTGCTC TTCAATCTGT CGCCAAGGGT GGGCGCGCCG 1141 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGAATCAA ATATCTTTTA GGCTAAAACA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt