Construct: ORF TRCN0000488941
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020558.1_s317c1
- DNA Barcode:
- CCCTATTACCTGCGAATGTTGACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TTBK2 (146057)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488941
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146057 | TTBK2 | tau tubulin kinase 2 | NM_173500.4 | 100% | 100% | |
| 2 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449849.1 | 100% | 100% | |
| 3 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449850.1 | 100% | 100% | |
| 4 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_005254173.5 | 94.4% | 94.3% | 0_1ins186;5delA;9_10ins22 |
| 5 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_006720403.4 | 94.3% | 94.2% | (many diffs) |
| 6 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_024449851.1 | 94.3% | 94.2% | (many diffs) |
| 7 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_006720402.4 | 92.7% | 92.9% | 1_133del;201_202ins148 |
| 8 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_017021950.2 | 92.5% | 92.5% | 0_1ins279 |
| 9 | human | 146057 | TTBK2 | tau tubulin kinase 2 | XM_005254171.5 | 91.9% | 92.3% | 1_166del;234_235ins148 |
| 10 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_001024856.2 | 88.8% | 89.7% | (many diffs) |
| 11 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_001024857.2 | 88.8% | 89.7% | (many diffs) |
| 12 | mouse | 140810 | Ttbk2 | tau tubulin kinase 2 | NM_080788.3 | 84.2% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3804
- ORF length:
- 3732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagtggg ggaggagagc agctggatat cctgagtgtt ggaatcctag 121 tgaaagaaag atggaaagtg ttgagaaaga ttgggggtgg gggctttgga gaaatttacg 181 atgccttgga catgctcacc agggaaaatg ttgcactgaa ggtggaatca gctcaacaac 241 caaaacaagt tctgaaaatg gaagttgctg ttttgaaaaa gctgcaaggg aaagaccatg 301 tttgtagatt tattggctgt gggaggaatg atcgattcaa ctatgtggtc atgcagttgc 361 agggtcggaa tctggcagat cttcgccgta gccagtcccg aggcacattc accattagta 421 ccactctccg gctgggtaga cagattttgg agtctattga aagcattcat tctgtgggat 481 tcttgcatcg agacatcaaa ccgtcgaact tcgctatggg tcgctttcct agtacatgta 541 ggaaatgtta catgcttgat tttggcttgg ctcgacaatt taccaattcc tgtggtgacg 601 tcagaccacc tcgagctgtg gcaggttttc gagggacagt tcgttatgca tcaatcaacg 661 cacatcggaa cagggaaatg ggaagacatg atgacctttg gtccttattc tacatgttgg 721 tggagtttgt ggttggtcag ctgccctgga gaaaaataaa ggacaaggag caagtaggct 781 ctattaagga gagatatgac cacaggctca tgttgaaaca tctccctcca gaattcagca 841 tctttctaga ccatatctct tctttggatt attttacaaa accagactac cagcttctta 901 catccgtgtt tgacaatagc atcaagactt ttggagtaat tgagagtgac ccttttgact 961 gggagaagac tggaaatgat ggctccctaa caaccaccac tacttctacc acccctcagt 1021 tgcacactcg cttgacccct gctgcaattg gaattgccaa tgctactccc atccctggag 1081 acttgcttcg agaaaataca gatgaggtat ttccagatga acagcttagc gatggagaaa 1141 atggcatccc tgttggtgtg tcaccagata aattgcctgg atctctggga cacccccgtc 1201 cccaggagaa ggatgtttgg gaagagatgg atgccaacaa aaacaagata aagcttggaa 1261 tttgtaaggc tgctactgaa gaggagaaca gccatggcca ggcaaatggt cttctcaatg 1321 ctccaagcct tgggtcacca attcgtgtcc gctcagagat tactcagcca gacagagata 1381 ttccactggt gcgaaagtta cgttccattc acagctttga gctggaaaaa cgtctgaccc 1441 tggagccaaa gccagacact gacaagttcc ttgagacctg cctggagaaa atgcagaaag 1501 ataccagtgc aggaaaagaa tctattctcc ctgctctgct gcataagcct tgcgttcctg 1561 ctgtgtcccg tactgaccac atctggcact atgatgaaga atatcttcca gatgcctcca 1621 agcctgcttc tgccaacacc cctgagcagg cagatggtgg tggcagcaat ggatttatag 1681 ctgttaacct gagctcttgc aagcaagaaa ttgattccaa agaatgggtg attgtggaca 1741 aggagcagga ccttcaggat tttaggacaa atgaggctgt aggacataaa acaactggaa 1801 gtccttctga tgaggagcct gaagtacttc aagtcctgga ggcatcacct caagatgaaa 1861 agctccagtt aggtccttgg gcagaaaatg atcatttaaa gaaggaaacc tcaggtgtgg 1921 tcttagcact ttctgcagag ggtcctccta ctgctgcttc agaacaatat acagataggc 1981 tggaactcca gcctggagct gctagtcagt ttattgcagc gacgcccaca agtctaatgg 2041 aggcgcaggc agaaggaccc cttacagcga ttacaattcc tagaccttct gtggcatcta 2101 cacagtcaac ttcaggaagc tttcactgtg gtcagcagcc agagaagaaa gatcttcagc 2161 ccatggagcc cactgtggaa ctttactctc caagggaaaa cttctctggc ttggttgtga 2221 cagagggtga acctcctagt ggaggaagca gaacagattt ggggcttcag atagatcaca 2281 ttggtcatga catgttaccc aacattagag aaagtaacaa atctcaagac ctgggaccaa 2341 aagaacttcc tgatcataat agactggttg tgagagaatt tgaaaatctc cctggggaaa 2401 ctgaagagaa aagcatcctt ttagagtcag ataatgaaga tgagaagtta agtagagggc 2461 agcattgtat tgagatctcc tctctcccag gagatttggt aattgtggaa aaggatcact 2521 cagctactac tgaacctctt gatgtgacaa aaacacagac ttttagtgtg gtgccaaatc 2581 aagacaaaaa taatgagata atgaagcttc tgacagttgg aacttcagaa atttcttcca 2641 gagacattga cccacatgtt gaaggtcaga taggccaagt ggcagaaatg caaaaaaata 2701 agatatctaa ggatgatgac atcatgagtg aagacttgcc aggtcatcaa ggagacctct 2761 ctactttttt gcaccaagag ggcaagagag agaaaatcac ccctagaaat ggagaactat 2821 ttcattgtgt ttcagagaat gaacatggtg ccccaacccg gaaggatatg gttaggtcat 2881 cctttgtaac tagacacagc cgaatccctg ttttagcaca agagatagac tcaactttgg 2941 aatcatcctc tccagtttct gcaaaagaaa agctcctcca aaagaaagcc tatcagccag 3001 acctagtcaa gcttctggtg gaaaaaagac aattcaagtc cttccttggc gacctctcaa 3061 gtgcctctga taaattgcta gaggagaaac tagctactgt tcctgctccc ttttgtgagg 3121 aggaagtgct cactcccttt tcaagactga cagtagattc tcacctgagt aggtcagctg 3181 aagatagctt tctgtcaccc atcatctccc agtctagaaa gagcaaaatt ccaaggccag 3241 tttcatgggt caacacagat caggtcaata gctcaacttc gtctcagttc tttcctcggc 3301 caccaccagg aaagccaccc acgaggcctg gagtagaagc caggctacgc agatataaag 3361 tcctagggag tagtaactcc gactcagacc ttttctcccg cctggcccaa attcttcaaa 3421 atggatctca gaaaccccGG AGCACTACTC AGTGCAAGAG TCCAGGATCT CCTCACAATC 3481 CAAAAACACC ACCCAAGAGT CCAGTTGTCC CTCGCAGGAG TCCCAGTGCC TCTCCTCGAA 3541 GCTCATCCTT GCCTCGCACG TCTAGTTCCT CACCATCTAG GGCTGGACGG CCCCACCATG 3601 ACCAGAGGAG TTCGTCCCCA CATCTGGGGA GAAGCAAGTC ACCTCCCAGC CACTCAGGAT 3661 CTTCCTCCTC CAGGAGGTCC TGCCAACAGG AGCATTGCAA ACCCAGCAAG AATGGCCTGA 3721 AAGGATCCGG CAGCCTCCAC CACCACTCAG CCAGCACTAA AACCCCCCAA GGGAAGAGTA 3781 AGCCAGCCAG TAAACTCAGC AGATAGACTA GTGACCCAGC TTTCTTGTAC AAAGTGGTTG 3841 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3901 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 3961 CTATTACCTG CGAATGTTGA CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 4021 ttgtgaaaga tt