Construct: ORF TRCN0000488961

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021627.1_s317c1
DNA Barcode:
GTCGGACGCGGTCTCTGATGACCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAP3K7 (6885)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488961
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6885 MAP3K7 mitogen-activated protein k... NM_145333.3 100% 100%
2 human 6885 MAP3K7 mitogen-activated protein k... NM_145332.3 94.7% 94.7% 1208_1288del
3 human 6885 MAP3K7 mitogen-activated protein k... NM_003188.4 84.8% 82.9% 1444_1559del;1590_1737del
4 human 6885 MAP3K7 mitogen-activated protein k... NM_145331.3 81% 79.3% 1208_1288del;1525_1640del;1671_1818del
5 human 6885 MAP3K7 mitogen-activated protein k... XM_017011226.2 62.3% 60.6% 0_1ins390;1054_1169del;1200_1347del
6 human 6885 MAP3K7 mitogen-activated protein k... XM_006715553.3 59.5% 57.9% (many diffs)
7 mouse 26409 Map3k7 mitogen-activated protein k... NM_172688.3 77.2% 82.3% (many diffs)
8 mouse 26409 Map3k7 mitogen-activated protein k... NM_009316.1 74% 78.6% (many diffs)
9 mouse 26409 Map3k7 mitogen-activated protein k... XM_006537948.3 54.7% 57.4% (many diffs)
10 mouse 26409 Map3k7 mitogen-activated protein k... XR_390323.3 20.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1542
ORF length:
1473
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gtctacagcc tctgccgcct cctcctcctc ctcgtcttcg gccggtgaga
121tgatcgaagc cccttcccag gtcctcaact ttgaagagat cgactacaag gagatcgagg
181tggaagaggt tgttggaaga ggagcctttg gagttgtttg caaagctaag tggagagcaa
241aagatgttgc tattaaacaa atagaaagtg aatctgagag gaaagcgttt attgtagagc
301ttcggcagtt atcccgtgtg aaccatccta atattgtaaa gctttatgga gcctgcttga
361atccagtgtg tcttgtgatg gaatatgctg aagggggctc tttatataat gtgctgcatg
421gtgctgaacc attgccatat tatactgctg cccacgcaat gagttggtgt ttacagtgtt
481cccaaggagt ggcttatctt cacagcatgc aacccaaagc gctaattcac agggacctga
541aaccaccaaa cttactgctg gttgcagggg ggacagttct aaaaatttgt gattttggta
601cagcctgtga cattcagaca cacatgacca ataacaaggg gagtgctgct tggatggcac
661ctgaagtttt tgaaggtagt aattacagtg aaaaatgtga cgtcttcagc tggggtatta
721ttctttggga agtgataacg cgtcggaaac cctttgatga gattggtggc ccagctttcc
781gaatcatgtg ggctgttcat aatggtactc gaccaccact gataaaaaat ttacctaagc
841ccattgagag cctgatgact cgttgttggt ctaaagatcc ttcccagcgc ccttcaatgg
901aggaaattgt gaaaataatg actcacttga tgcggtactt tccaggagca gatgagccat
961tacagtatcc ttgtcagtat tcagatgaag gacagagcaa ctctgccacc agtacaggct
1021cattcatgga cattgcttct acaaatacga gtaacaaaag tgacactaat atggagcaag
1081ttcctgccac aaatgatact attaagcgct tagaatcaaa attgttgaaa aatcaggcaa
1141agcaacagag tgaatctgga cgtttaagct tgggagcctc ccgtgGGAGC AGTGTGGAGA
1201GCTTGCCCCC AACCTCTGAG GGCAAGAGGA TGAGTGCTGA CATGTCTGAA ATAGAAGCTA
1261GGATCGCCGC AACCACAGGC AACGGACAGC CAAGACGTAG ATCCATCCAA GACTTGACTG
1321TAACTGGAAC AGAACCTGGT CAGGTGAGCA GTAGGTCATC CAGTCCCAGT GTCAGAATGA
1381TTACTACCTC AGGACCAACC TCAGAAAAGC CAACTCGAAG TCATCCATGG ACCCCTGATG
1441ATTCCACAGA TACCAATGGA TCAGATAACT CCATCCCAAT GGCTTATCTT ACACTGGATC
1501ACCAACTACA GGCAAGAACT AGTTGCAGAA CTGGACCAGG ATAGAACCCA GCTTTCTTGT
1561ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1621AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1681GAAAGGACGA GTCGGACGCG GTCTCTGATG ACCGACGCGT TAAGTCgaca atcaacctct
1741ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)