Construct: ORF TRCN0000488962
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021560.1_s317c1
- DNA Barcode:
- GTTCGGAGAGGCTCTATGGTTGGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DUSP10 (11221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488962
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11221 | DUSP10 | dual specificity phosphatas... | NM_007207.6 | 99.9% | 100% | 294C>A |
| 2 | human | 11221 | DUSP10 | dual specificity phosphatas... | XM_017000147.2 | 57.3% | 56.4% | (many diffs) |
| 3 | human | 11221 | DUSP10 | dual specificity phosphatas... | NR_111940.1 | 30.5% | (many diffs) | |
| 4 | human | 11221 | DUSP10 | dual specificity phosphatas... | NR_111939.1 | 27.3% | (many diffs) | |
| 5 | mouse | 63953 | Dusp10 | dual specificity phosphatas... | NM_022019.5 | 92% | 96.4% | (many diffs) |
| 6 | mouse | 63953 | Dusp10 | dual specificity phosphatas... | XM_006497188.2 | 92% | 96.4% | (many diffs) |
| 7 | mouse | 63953 | Dusp10 | dual specificity phosphatas... | XM_006497189.1 | 92% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1518
- ORF length:
- 1446
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcctccg tctcctttag acgacagggt agtagtggca ctatctaggc 121 ccgtccgacc tcaggatctc aacctttgtt tagactctag ttaccttggc tctgccaacc 181 caggcagtaa cagccaccct cctgtcatcg ccaccaccgt tgtgtccctc aaggctgcga 241 atctgacgta tatgccctca tccagcggct ctgcccgctc gctgaattgt ggatgcagca 301 gtgccagctg ctgcactgtg gcaacctacg acaaggacaa tcaggcccaa acccaagcca 361 ttgcagctgg caccaccacc actgccatcg gaacctctac cacctgccct gctaaccaga 421 tggtcaacaa taatgagaat acaggctctc taagtccatc aagtggggtg ggcagccctg 481 tgtcagggac ccccaagcag ctagccagca tcaaaataat ctaccccaat gacttggcaa 541 agaagatgac caaatgcagc aagagtcacc tgccgagtca gggccctgtc atcattgact 601 gcaggccctt catggagtac aacaagagtc acatccaagg agctgtccac attaactgtg 661 ccgataagat cagccggcgg agactgcagc agggcaagat cactgtccta gacttgattt 721 cctgtaggga aggcaaggac tctttcaaga ggatcttttc caaagaaatt atagtttatg 781 atgagaatac caatgaacca agccgagtga tgccctccca gccacttcac atagtcctcg 841 agtccctgaa gagagaaggc aaagaacctc tggtgttgaa aggtggactt agtagtttta 901 agcagaacca tgaaaacctc tgtgacaact ccctccagct ccaagagtgc cgggaggtgg 961 ggggcggcgc atccgcggcc tcgagcttgc tacctcagcc catccccacc acccctgaca 1021 tcgagaacgc tgagctcacc cccatcttgc ccttcctgtt ccttggcaat gagcaggatg 1081 ctcaggacct ggacaccatg cagcggctga acatcggcta cgtcatcaac gtcaccactc 1141 atcttcccct ctaccactat gagaaaggcc tgttcaacta caagcggctg ccagccactg 1201 acagcaacaa gcagaaccTG CGGCAGTACT TTGAAGAGGC TTTTGAGTTC ATTGAGGAAG 1261 CTCACCAGTG TGGGAAGGGG CTTCTCATCC ACTGCCAGGC TGGGGTGTCC CGCTCCGCCA 1321 CCATCGTCAT CGCTTACTTG ATGAAGCACA CTCGGATGAC CATGACTGAT GCTTATAAAT 1381 TTGTCAAAGG CAAACGACCA ATTATCTCCC CAAACCTTAA CTTCATGGGG CAGTTGCTAG 1441 AGTTCGAGGA AGACCTAAAC AACGGTGTGA CACCGAGAAT CCTTACACCA AAGCTGATGG 1501 GCGTGGAGAC GGTTGTGTAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTC GGAGAGGCTC 1681 TATGGTTGGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt