Construct: ORF TRCN0000488963
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021575.1_s317c1
- DNA Barcode:
- AACAAACATTACAGCATACCTACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MTM1 (4534)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488963
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4534 | MTM1 | myotubularin 1 | NM_000252.2 | 99.9% | 99.8% | 1228G>A |
| 2 | human | 4534 | MTM1 | myotubularin 1 | XM_005274687.2 | 99.9% | 99.8% | 1228G>A |
| 3 | human | 4534 | MTM1 | myotubularin 1 | XM_011531173.2 | 99.9% | 99.8% | 1228G>A |
| 4 | human | 4534 | MTM1 | myotubularin 1 | XM_017029549.1 | 99.7% | 99.6% | 1228G>A;1644_1645insCAG |
| 5 | human | 4534 | MTM1 | myotubularin 1 | XM_011531171.1 | 97.5% | 97.4% | 231_275del;1273G>A |
| 6 | human | 4534 | MTM1 | myotubularin 1 | XM_011531172.1 | 97.5% | 97.4% | 231_275del;1273G>A |
| 7 | human | 4534 | MTM1 | myotubularin 1 | XM_017029548.1 | 97.5% | 97.4% | 231_275del;1273G>A |
| 8 | human | 4534 | MTM1 | myotubularin 1 | XM_017029547.1 | 97.3% | 97.2% | 231_275del;1273G>A;1689_1690insCAG |
| 9 | human | 4534 | MTM1 | myotubularin 1 | XM_017029550.1 | 93.8% | 93.6% | 231_232ins111;1117G>A |
| 10 | human | 4534 | MTM1 | myotubularin 1 | XM_017029551.2 | 58.8% | 58.7% | 0_1ins744;484G>A |
| 11 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_001164190.1 | 88.7% | 91.8% | (many diffs) |
| 12 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_001164191.1 | 88.7% | 91.8% | (many diffs) |
| 13 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_019926.3 | 88.7% | 91.8% | (many diffs) |
| 14 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_001164192.1 | 84.1% | 86.8% | (many diffs) |
| 15 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | XM_006527890.1 | 80.1% | 82.5% | (many diffs) |
| 16 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | XM_006527891.1 | 74.6% | 78.1% | (many diffs) |
| 17 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | XM_017318416.1 | 63.8% | 65.9% | (many diffs) |
| 18 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_001164193.1 | 23.4% | 21.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1881
- ORF length:
- 1809
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcttct gcatcaactt ctaaatataa ttcacactcc ttggagaatg 121 agtctattaa gaggacgtct cgagatggag tcaatcgaga tctcactgag gctgttcctc 181 gacttccagg agaaacacta atcactgaca aagaagttat ttacatatgt cctttcaatg 241 gccccattaa gggaagagtt tacatcacaa attatcgtct ttatttaaga agtttggaaa 301 cggattcttc tctaatactt gatgttcctc tgggtgtgat ctcgagaatt gaaaaaatgg 361 gaggcgcgac aagtagagga gaaaattcct atggtctaga tattacttgt aaagacatga 421 gaaacctgag gttcgctttg aaacaggaag gccacagcag aagagatatg tttgagatcc 481 tcacgagata cgcgtttccc ctggctcaca gtctgccatt atttgcattt ttaaatgaag 541 aaaagtttaa cgtggatgga tggacagttt acaatccagt ggaagaatac aggaggcagg 601 gcttgcccaa tcaccattgg agaataactt ttattaataa gtgctatgag ctctgtgaca 661 cttaccctgc tcttttggtg gttccgtatc gtgcctcaga tgatgacctc cggagagttg 721 caacttttag gtcccgaaat cgaattccag tgctgtcatg gattcatcca gaaaataaga 781 cggtcattgt gcgttgcagt cagcctcttg tcggtatgag tgggaaacga aataaagatg 841 atgagaaata tctcgatgtt atcagggaga ctaataaaca aatttctaaa ctcaccattt 901 atgatgcaag acccagcgta aatgcagtgg ccaacaaggc aacaggagga ggatatgaaa 961 gtgatgatgc atatcataac gccgaacttt tcttcttaga cattcataat attcatgtta 1021 tgcgggaatc tttaaaaaaa gtgaaggaca ttgtttatcc taatgtagaa gaatctcatt 1081 ggttgtccag tttggagtct actcattggt tagaacatat caagctcgtt ttgacaggag 1141 ccattcaagt agcagacaaa gtttcttcag ggaagagttc agtgcttgtg cattgcagtg 1201 acggatggga caggactgct cagctgacat ccttggccat gctgatgttg gatagcttct 1261 ataggagcat tgaagggttc gaaatactgg tacaaaaaaa atggataagt tttggacata 1321 aatttgcatc tcgaataggt catggtgata aaaaccacac cgatgctgac cgttctccta 1381 tttttctcca gtttattgat tgtgtgtggc aaatgtcaaa acagttccct acagcttttg 1441 aattcaatga acaatttttg attataattt tggatcatct gtatagttgc cgatttggta 1501 ctttcttatt caactgtgaa tctgctcgag aaaGACAGAA GGTTACAGAA AGGACTGTTT 1561 CTTTATGGTC ACTGATAAAC AGTAATAAAG AAAAATTCAA AAACCCCTTC TATACTAAAG 1621 AAATCAATCG AGTTTTATAT CCAGTTGCCA GTATGCGTCA CTTGGAACTC TGGGTGAATT 1681 ACTACATTAG ATGGAACCCC AGGATCAAGC AACAACAGCC GAATCCAGTG GAGCAGCGTT 1741 ACATGGAGCT CTTAGCCTTA CGCGACGAAT ACATAAAGCG GCTTGAGGAA CTGCAGCTCG 1801 CCAACTCTGC CAAGCTTTCT GATCCCCCAA CTTCACCTTC CAGTCCTTCG CAAATGATGC 1861 CCCATGTGCA AACTCACTTC TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1921 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1981 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ACAAACATTA 2041 CAGCATACCT ACGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2101 att