Construct: ORF TRCN0000488977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019291.1_s317c1
- DNA Barcode:
- TTTGGGGCAATGTCTCGAAATCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGR6 (59352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488977
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_021636.3 | 99.9% | 99.8% | 2745_2746insG |
| 2 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_005245404.3 | 97.3% | 95.7% | (many diffs) |
| 3 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509838.2 | 94.7% | 93.1% | (many diffs) |
| 4 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509839.2 | 94.7% | 93.1% | (many diffs) |
| 5 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_001017403.1 | 93.3% | 92.5% | (many diffs) |
| 6 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001996.1 | 92.1% | 90.5% | (many diffs) |
| 7 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509841.2 | 90.7% | 89.9% | (many diffs) |
| 8 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001997.1 | 90.6% | 90% | (many diffs) |
| 9 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509840.2 | 89.6% | 88% | (many diffs) |
| 10 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_001017404.1 | 87% | 85.5% | (many diffs) |
| 11 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509842.2 | 84.8% | 84.8% | 0_1ins414;2331_2332insG |
| 12 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509843.2 | 84.8% | 84.8% | 0_1ins414;2331_2332insG |
| 13 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509846.2 | 84.8% | 84.8% | 0_1ins414;2331_2332insG |
| 14 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509844.2 | 77.1% | 76.9% | (many diffs) |
| 15 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001998.1 | 66.3% | 64.8% | (many diffs) |
| 16 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_011248036.2 | 84.1% | 88.2% | (many diffs) |
| 17 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | NM_001033409.3 | 80.6% | 84.5% | (many diffs) |
| 18 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_006529714.1 | 78.3% | 82.1% | (many diffs) |
| 19 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_017321280.1 | 75% | 79.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2823
- ORF length:
- 2748
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggga agaccaaggt tgacacttgt ttgtcaagtg tcaataatca 121 tctctgcccg ggacctcagc atgaacaacc tcacagagct tcagcctggc ctcttccacc 181 acctgcgctt cttggaggag ctgcgtctct ctgggaacca tctctcacac atcccaggac 241 aagcattctc tggtctctac agcctgaaaa tcctgatgct gcagaacaat cagctgggag 301 gaatccccgc agaggcgctg tgggagctgc cgagcctgca gtcgctgcgc ctagatgcca 361 acctcatctc cctggtcccg gagaggagct ttgaggggct gtcctccctc cgccacctct 421 ggctggacga caatgcactc acggagatcc ctgtcagggc cctcaacaac ctccctgccc 481 tgcaggccat gaccctggcc ctcaaccgca tcagccacat ccccgactac gcgttccaga 541 atctcaccag ccttgtggtg ctgcatttgc ataacaaccg catccagcat ctggggaccc 601 acagcttcga ggggctgcac aatctggaga cactagacct gaattataac aagctgcagg 661 agttccctgt ggccatccgg accctgggca gactgcagga actggggttc cataacaaca 721 acatcaaggc catcccagaa aaggccttca tggggaaccc tctgctacag acgatacact 781 tttatgataa cccaatccag tttgtgggaa gatcggcatt ccagtacctg cctaaactcc 841 acacactatc tctgaatggt gccatggaca tccaggagtt tccagatctc aaaggcacca 901 ccagcctgga gatcctgacc ctgacccgcg caggcatccg gctgctccca tcggggatgt 961 gccaacagct gcccaggctc cgagtcctgg aactgtctca caatcaaatt gaggagctgc 1021 ccagcctgca caggtgtcag aaattggagg aaatcggcct ccaacacaac cgcatctggg 1081 aaattggagc tgacaccttc agccagctga gctccctgca agccctggat cttagctgga 1141 acgccatccg gtccatccac cccgaggcct tctccaccct gcactccctg gtcaagctgg 1201 acctgacaga caaccagctg accacactgc ccctggctgg acttgggggc ttgatgcatc 1261 tgaagctcaa agggaacctt gctctctccc aggccttctc caaggacagt ttcccaaaac 1321 tgaggatcct ggaggtgcct tatgcctacc agtgctgtcc ctatgggatg tgtgccagct 1381 tcttcaaggc ctctgggcag tgggaggctg aagaccttca ccttgatgat gaggagtctt 1441 caaaaaggcc cctgggcctc cttgccagac aagcagagaa ccactatgac caggacctgg 1501 atgagctcca gctggagatg gaggactcaa agccacaccc cagtgtccag tgtagcccta 1561 ctccaggccc cttcaagccc tgtgagtacc tctttgaaag ctggggcatc cgcctggccg 1621 tgtgggccat cgtgttgctc tccgtgctct gcaatggact ggtgctgctg accgtgttcg 1681 ctggcgggcc tgtccccctg cccccggtca agtttgtggt aggtgcgatt gcaggcgcca 1741 acaccttgac tggcatttcc tgtggccttc tagcctcagt cgatgccctg acctttggtc 1801 agttctctga gtacggagcc cgctgggaga cggggctagg ctgccgggcc actggcttcc 1861 tggcagtact tgggtcggag gcatcggtgc tgctgctcac tctggccgca gtgcagtgca 1921 gcgtctccgt ctcctgtgtc cgggcctatg ggaagtcccc ctccctgggc agcgttcgag 1981 caggggtcct aggctgcctg gcactggcag ggctggccgc cgcgctgccc ctggcctcag 2041 tgggagaata cggggcctcc ccactctgcc tgccctacgc gccacctgag ggtcagccag 2101 cagccctggg cttcaccgtg gccctggtga tgatgaactc cttctgtttc ctggtcgtgg 2161 ccggtgccta catcaaactg tactgtgacc tgccgcgggg cgactttgag gccgtgtggg 2221 actgcgccat ggtgaggcac gtggcctggc tcatcttcgc agacgggctc ctctactgtc 2281 ccgtggcctt cctcagcttt gcctccatgc tgggcctctt ccctgtcacg cccgaggccg 2341 tcaagtctgt cctgctggtg gtgctgcccc tgcctgcctg cctcaaccca ctgctgtacc 2401 tgctcttcaa cccccacttc cgggatgacc ttcggcggct tcggccccgc gcaggggact 2461 cagggcccct agcctatgct gcggccgggg agctggagaa gagctcctgt gattctaccc 2521 aggccctggt agCCTTCTCT GATGTGGATC TCATTCTGGA AGCTTCTGAA GCTGGGCGGC 2581 CCCCTGGGCT GGAGACCTAT GGCTTCCCCT CAGTGACCCT CATCTCCTGT CAGCAGCCAG 2641 GGGCCCCCAG GCTGGAGGGC AGCCATTGTG TAGAGCCAGA GGGGAACCAC TTTGGGAACC 2701 CCCAACCCTC CATGGATGGA GAACTGCTGC TGAGGGCAGA GGGATCTACG CCAGCAGGTG 2761 GAGGCTTGTC AGGGGGTGGC GGCTTTCAGC CCTCTGGCTT GGCCTTTGCT TCACACGTGG 2821 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2881 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2941 TTTATATATC TTGTGGAAAG GACGATTTGG GGCAATGTCT CGAAATCGTA CGCGTTAAGT 3001 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt