Construct: ORF TRCN0000488991
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021874.1_s317c1
- DNA Barcode:
- TATGTTCAGGTTCGCAGAACGATA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HK2 (3099)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488991
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3099 | HK2 | hexokinase 2 | NM_000189.5 | 99.9% | 100% | 2298A>G |
| 2 | human | 3099 | HK2 | hexokinase 2 | XM_005264280.2 | 97.7% | 97.7% | 1838_1900del;2361A>G |
| 3 | human | 3099 | HK2 | hexokinase 2 | NM_001371525.1 | 96.9% | 96.9% | 0_1ins84;2214A>G |
| 4 | human | 3099 | HK2 | hexokinase 2 | XM_017003945.2 | 96.4% | 96.5% | 494_495ins96;2202A>G |
| 5 | human | 3099 | HK2 | hexokinase 2 | XM_011532807.2 | 94.3% | 94.3% | 494_495ins96;1742_1804del;2265A>G |
| 6 | mouse | 15277 | Hk2 | hexokinase 2 | NM_013820.3 | 87.4% | 94.2% | (many diffs) |
| 7 | mouse | 15277 | Hk2 | hexokinase 2 | XM_017321401.1 | 77.2% | 83.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2820
- ORF length:
- 2751
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gattgcctcg catctgcttg cctacttctt cacggagctc aaccatgacc 121 aagtgcagaa ggttgaccag tatctctacc acatgcgcct ctctgatgag accctcttgg 181 agatctctaa gcggttccgc aaggagatgg agaaagggct tggagccacc actcacccta 241 ctgcagcagt gaagatgctg cccacctttg tgaggtccac tccagatggg acagaacacg 301 gagagttcct ggctctggat cttggaggga ccaacttccg tgtgctttgg gtgaaagtaa 361 cggacaatgg gctccagaag gtggagatgg agaatcagat ctatgccatc cctgaggaca 421 tcatgcgagg cagtggcacc cagctgtttg accacattgc cgaatgcctg gctaacttca 481 tggataagct acaaatcaaa gacaagaagc tcccactggg ttttaccttc tcgttcccct 541 gccaccagac taaactagac gagagtttcc tggtctcatg gaccaaggga ttcaagtcca 601 gtggagtgga aggcagagac gttgtggctc tgatccggaa ggccatccag aggagagggg 661 actttgatat cgacattgtg gctgtggtga atgacacagt tgggaccatg atgacctgtg 721 gttatgatga ccacaactgt gagattggtc tcattgtggg cacgggcagc aacgcctgct 781 acatggaaga gatgcgccac atcgacatgg tggaaggcga tgaggggcgg atgtgtatca 841 atatggagtg gggggccttc ggggacgatg gctcgctcaa cgacattcgc actgagtttg 901 accaggagat tgacatgggc tcactgaacc cgggaaagca actgtttgag aagatgatca 961 gtgggatgta catgggggag ctggtgaggc ttatcctggt gaagatggcc aaggaggagc 1021 tgctctttgg ggggaagctc agcccagagc ttctcaacac cggtcgcttt gagaccaaag 1081 acatctcaga cattgaaggg gagaaggatg gcatccggaa ggcccgtgag gtcctgatgc 1141 ggttgggcct ggacccgact caggaggact gcgtggccac tcaccggatc tgccagatcg 1201 tgtccacacg ctccgccagc ctgtgcgcag ccaccctggc cgccgtgctg cagcgcatca 1261 aggagaacaa aggcgaggag cggctgcgct ctactattgg ggtcgacggt tccgtctaca 1321 agaaacaccc ccattttgcc aagcgtctac ataagaccgt gcggcggctg gtgcccggct 1381 gcgatgtccg cttcctccgc tccgaggatg gcagtggcaa aggtgcagcc atggtgacag 1441 cagtggctta ccggctggcc gatcaacacc gtgcccgcca gaagacatta gagcatctgc 1501 agctgagcca tgaccagctg ctggaggtca agaggaggat gaaggtagaa atggagcgag 1561 gtctgagcaa ggagactcat gccagtgccc ccgtcaagat gctgcccacc tacgtgtgtg 1621 ctaccccgga cggcacagag aaaggggact tcttggcctt ggaccttgga ggaacaaatt 1681 tccgggtcct gctggtccgt gttcggaatg ggaagtgggg tggagtggag atgcacaaca 1741 agatctacgc catcccgcag gaggtcatgc acggcaccgg ggacgagctc tttgaccaca 1801 ttgtccagtg catcgcggac ttcctcgagt acatgggcat gaagggcgtg tccctgcctc 1861 tgggttttac cttctccttc ccctgccagc agaacagcct ggacgagagc atcctcctca 1921 agtggacaaa aggcttcaag gcatctggct gcgagggcga ggacgtggtg accctgctga 1981 aggaagcgat ccaccggcga gaggagtttg acctggatgt ggttgctgtg gtgaacgaca 2041 cagtcggaac tatgatgacc tgtggctttg aagaccctca ctgtgaagtt ggcctcattg 2101 ttggcacggg cagcaatgcc tgctacatgg aggagatgcg caacgtggaa ctggtggaag 2161 gagaagaggg gcggatgtgt gtgaacatgg aatggggggc cttcggggac aatggatgcc 2221 tagatgactt ccgcacagaa tttgatgtgg ctgtggatga gctttcactc aaccccggca 2281 agcagaggtt cgagaaaatg atcagtggaa tgtacctggg tgagattgtc cgtaacattc 2341 tcatcgattt caccaagcgt ggactgctct tccgaggccg catctcagag cggctcaaga 2401 caaggggcat ctttgaaacc aagttcttgt ctcagattga gagtgactgc ctggccctgc 2461 tgcaagtccg agccatcctg caacacttag ggcttgagag caccTGTGAC GACAGCATCA 2521 TTGTTAAGGA GGTGTGCACT GTGGTGGCCC GGCGGGCAGC CCAGCTCTGT GGCGCAGGCA 2581 TGGCCGCTGT GGTGGACAGG ATACGAGAAA ACCGTGGGCT GGACGCTCTC AAAGTGACAG 2641 TGGGTGTGGA TGGGACCCTC TACAAGCTAC ATCCTCACTT TGCCAAAGTC ATGCATGAGA 2701 CAGTGAAGGA CCTGGCTCCG AAATGTGATG TGTCTTTCCT GCAGTCAGAG GATGGCAGCG 2761 GGAAGGGGGC GGCGCTCATC ACTGCTGTGG CCTGCCGCAT CCGTGAGGCT GGACAGCGAT 2821 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2881 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2941 GGCTTTATAT ATCTTGTGGA AAGGACGATA TGTTCAGGTT CGCAGAACGA TAACGCGTTA 3001 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt