Construct: ORF TRCN0000488998
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021245.1_s317c1
- DNA Barcode:
- GATTCCACCCGGTGTCTGGGTCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ECE2 (9718)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488998
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 110599564 | EEF1AKMT4 | EEF1A lysine methyltransfer... | NM_032331.3 | 99.8% | 99.6% | 301C>T |
| 2 | human | 110599583 | EEF1AKMT4-ECE2 | EEF1AKMT4-ECE2 readthrough | NM_014693.4 | 25% | 19.5% | (many diffs) |
| 3 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | NM_025462.2 | 85.3% | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 837
- ORF length:
- 765
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcctct ccaggggcag gtagggcgcc tccggagtta ccggagcgga 121 actgcgggta ccgcgaagtc gagtactggg atcagcgcta ccaaggcgca gccgattctg 181 ccccctacga ttggttcggg gacttctcct ccttccgtgc cctcctagag ccggagctgc 241 ggcccgagga ccgtatcctt gtgctaggtt gcgggaacag tgccctgagc tacgagctgt 301 tcctcggagg cttccctaat gtgaccagtg tggactactc atcagtcgtg gtggctgcca 361 tgcaggctcg ctatgcccat gtgccgcagc tgcgctggga gaccatggat gtgcggaagc 421 tggacttccc cagtgcttct tttgatgtgg tgctcgagaa gggcacgctg gatgcccTGC 481 TGGCTGGGGA ACGAGATCCC TGGACCGTGT CCTCTGAAGG TGTCCACACT GTGGACCAGG 541 TGTTGAGTGA GGTGAGCCGC GTGCTTGTCC CTGGAGGCCG GTTTATCTCA ATGACTTCTG 601 CTGCCCCCCA CTTTCGGACC AGACACTATG CCCAAGCCTA TTATGGCTGG TCCCTGAGGC 661 ATGCTACCTA TGGCAGCGGT TTCCACTTCC ATCTCTACCT CATGCACAAG GGCGGGAAGC 721 TCAGTGTGGC CCAGCTGGCT CTGGGGGCCC AAATCCTCTC ACCCCCCAGA CCTCCCACCT 781 CACCTTGCTT CCTTCAGGAC TCAGATCATG AGGACTTCCT TAGTGCCATT CAGCTCTAGA 841 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGAGATTC CACCCGGTGT CTGGGTCAAA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt