Construct: ORF TRCN0000489002
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020709.1_s317c1
- DNA Barcode:
- TGTACTAACTGGCCAATTCCGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKD2 (25865)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489002
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079880.1 | 100% | 100% | |
| 2 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079881.1 | 100% | 100% | |
| 3 | human | 25865 | PRKD2 | protein kinase D2 | NM_016457.4 | 100% | 100% | |
| 4 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079882.1 | 82.1% | 82.1% | 0_1ins471 |
| 5 | human | 25865 | PRKD2 | protein kinase D2 | XM_005258716.2 | 82.1% | 82.1% | 0_1ins471 |
| 6 | mouse | 101540 | Prkd2 | protein kinase D2 | NM_001252458.1 | 88.2% | 95.1% | (many diffs) |
| 7 | mouse | 101540 | Prkd2 | protein kinase D2 | NM_178900.4 | 88.2% | 95.1% | (many diffs) |
| 8 | mouse | 101540 | Prkd2 | protein kinase D2 | XR_391036.1 | 60.5% | (many diffs) | |
| 9 | mouse | 101540 | Prkd2 | protein kinase D2 | XM_006539444.1 | 55.4% | 59.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2706
- ORF length:
- 2634
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccacc gccccctctt atcccgccgg gctccctggc tctcccgggc 121 cggggtctcc tccgcccccc ggcggcctag agctgcagtc gccgccaccg ctactgcccc 181 agatcccggc cccgggttcc ggggtctcct ttcacatcca gatcgggctg acccgcgagt 241 tcgtgctgtt gcccgccgcc tccgagctgg ctcatgtgaa gcagctggcc tgttccatcg 301 tggaccagaa gttccctgag tgtggcttct acggccttta cgacaagatc ctgcttttca 361 aacatgaccc cacgtcggcc aacctcctgc agctggtgcg ctcgtccgga gacatccagg 421 agggcgacct ggtggaggtg gtgctgtcgg cctcggccac cttcgaggac ttccagatcc 481 gcccgcacgc cctcacggtg cactcctatc gggcgcctgc cttctgtgat cactgcgggg 541 agatgctctt cggcctagtg cgccagggcc tcaagtgcga tggctgcggg ctgaactacc 601 acaagcgctg tgccttcagc atccccaaca actgtagtgg ggcccgcaaa cggcgcctgt 661 catccacgtc tctggccagt ggccactcgg tgcgcctcgg cacctccgag tccctgccct 721 gcacggctga agagctgagc cgtagcacca ccgaactcct gcctcgccgt cccccgtcat 781 cctcttcctc ctcttctgcc tcatcgtata cgggccgccc cattgagctg gacaagatgc 841 tgctctccaa ggtcaaggtg ccgcacacct tcctcatcca cagctataca cggcccaccg 901 tttgccaggc ttgcaagaaa ctcctcaagg gcctcttccg gcagggcctg caatgcaaag 961 actgcaagtt taactgtcac aaacgctgcg ccacccgcgt ccctaatgac tgcctggggg 1021 aggcccttat caatggagat gtgccgatgg aggaggccac cgatttcagc gaggctgaca 1081 agagcgccct catggatgag tcagaggact ccggtgtcat ccctggctcc cactcagaga 1141 atgcgctcca cgccagtgag gaggaggaag gcgagggagg caaggcccag agctccctgg 1201 ggtacatccc cctaatgagg gtggtgcaat cggtgcgaca cacgacgcgg aaatccagca 1261 ccacgctgcg ggagggttgg gtggttcatt acagcaacaa ggacacgctg agaaagcggc 1321 actattggcg cctggactgc aagtgtatca cgctcttcca gaacaacacg accaacagat 1381 actataagga aattccgctg tcagaaatcc tcacggtgga gtccgcccag aacttcagcc 1441 ttgtgccgcc gggcaccaac ccacactgct ttgagatcgt cactgccaat gccacctact 1501 tcgtgggcga gatgcctggc gggactccgg gtgggccaag tgggcagggg gctgaggccg 1561 cccggggctg ggagacagcc atccgccagg ccctgatgcc cgtcatcctt caggacgcac 1621 ccagcgcccc aggccacgcg ccccacagac aagcttctct gagcatctct gtgtccaaca 1681 gtcagatcca agagaatgtg gacattgcca ctgtctacca gatcttccct gacgaagtgc 1741 tgggctcagg gcagtttgga gtggtctatg gaggaaaaca ccggaagaca ggccgggacg 1801 tggcagttaa ggtcattgac aaactgcgct tccctaccaa gcaggagagc cagctccgga 1861 atgaagtggc cattctgcag agcctgcggc atcccgggat cgtgaacctg gagtgcatgt 1921 tcgagacgcc tgagaaagtg tttgtggtga tggagaagct gcatggggac atgttggaga 1981 tgatcctgtc cagtgagaag ggccggctgc ctgagcgcct caccaagttc ctcatcaccc 2041 agatcctggt ggctttgaga caccttcact tcaagaacat tgtccactgt gacttgaaac 2101 cagaaaacgt gttgctggca tcagcagacc catttcctca ggtgaagctg tgtgactttg 2161 gctttgctcg catcatcggc gagaagtcgt tccgccgctc agtggtgggc acgccggcct 2221 acctggcacc cgaggtgctg ctcaaccagg gctacaaccg ctcgctggac atgtggtcag 2281 tgggcgtgat catgtacgtc agcctcagcg gcaccTTCCC TTTCAACGAG GATGAGGACA 2341 TCAATGACCA GATCCAGAAC GCCGCCTTCA TGTACCCGGC CAGCCCCTGG AGCCACATCT 2401 CAGCTGGAGC CATTGACCTC ATCAACAACC TGCTGCAGGT GAAGATGCGC AAACGCTACA 2461 GCGTGGACAA ATCTCTCAGC CACCCCTGGT TACAGGAGTA CCAGACGTGG CTGGACCTCC 2521 GAGAGCTGGA GGGGAAGATG GGAGAGCGAT ACATCACGCA TGAGAGTGAC GACGCGCGCT 2581 GGGAGCAGTT TGCAGCAGAG CATCCGCTGC CTGGGTCTGG GCTGCCCACG GACAGGGATC 2641 TCGGTGGGGC CTGTCCACCA CAGGACCACG ACATGCAGGG GCTGGCGGAG CGCATCAGTG 2701 TTCTCTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2761 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2821 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGTACT AACTGGCCAA TTCCGCACAC 2881 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt